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Volumn 27, Issue 3, 2004, Pages 140-146

The neutral comet assay detects double strand DNA damage in selected and unselected human spermatosoa of normospermic donors

Author keywords

Comet assay; DNA damage; Human sperm; Irradiation; Sperm selection

Indexed keywords

DOUBLE STRANDED DNA;

EID: 2642562899     PISSN: 01056263     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2605.2004.00463.x     Document Type: Article
Times cited : (34)

References (51)
  • 1
    • 0028305534 scopus 로고
    • The effect of various antioxidants and other modifying agents on oxygen radical generated DNA-damage in human lymphocytes in the comet assay
    • Anderson, D., Yu, T. -W., Phillips, B. J. & Schmezer, P. (1994) The effect of various antioxidants and other modifying agents on oxygen radical generated DNA-damage in human lymphocytes in the comet assay. Mutation Research 307, 261-271.
    • (1994) Mutation Research , vol.307 , pp. 261-271
    • Anderson, D.1    Yu, T.W.2    Phillips, B.J.3    Schmezer, P.4
  • 2
    • 0031563793 scopus 로고    scopus 로고
    • Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single-cell electrophoresis
    • Aravindan, G. R., Bjordahl, J., Jost, L. K. & Evenson, D. P. (1997) Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single-cell electrophoresis. Experimental Cell Research 236, 231-237.
    • (1997) Experimental Cell Research , vol.236 , pp. 231-237
    • Aravindan, G.R.1    Bjordahl, J.2    Jost, L.K.3    Evenson, D.P.4
  • 4
    • 0015611906 scopus 로고
    • Variations in structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa
    • Bedford, J. M., Bent, M. J. & Calvin, H. (1973) Variations in structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa. Journal of Reproduction and Fertility 33, 19-29.
    • (1973) Journal of Reproduction and Fertility , vol.33 , pp. 19-29
    • Bedford, J.M.1    Bent, M.J.2    Calvin, H.3
  • 5
    • 0036798423 scopus 로고    scopus 로고
    • Prenatal testing in ICSI pregnancies: Incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters
    • Bonduelle, M., van Assche, E., Joris, H., Keymolen, K., Devroey, P., Van Steirteghem, A. & Liebaers, I. (2002) Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters. Human Reproduction 17, 2600-2614.
    • (2002) Human Reproduction , vol.17 , pp. 2600-2614
    • Bonduelle, M.1    Van Assche, E.2    Joris, H.3    Keymolen, K.4    Devroey, P.5    Van Steirteghem, A.6    Liebaers, I.7
  • 7
    • 0029738401 scopus 로고    scopus 로고
    • Expression and localisation of DNA topoisomerase II during rat spermatogenesis
    • Chen, J. L. & Longo, F. J. (1996) Expression and localisation of DNA topoisomerase II during rat spermatogenesis. Molecular Reproduction and Development 45, 61-71.
    • (1996) Molecular Reproduction and Development , vol.45 , pp. 61-71
    • Chen, J.L.1    Longo, F.J.2
  • 8
    • 0034303523 scopus 로고    scopus 로고
    • The origins, patterns and implications of human spontaneous mutation
    • Crow, J. F. (2000) The origins, patterns and implications of human spontaneous mutation. Nature Reviews Genetics 1, 40-47.
    • (2000) Nature Reviews Genetics , vol.1 , pp. 40-47
    • Crow, J.F.1
  • 9
    • 2642516049 scopus 로고    scopus 로고
    • Spermatozoa DNA-integrity: Correlation with fertilization in vitro and pregnancy rates in IVF and ICSI
    • abstract book 1
    • Donnelly, E. T., McClure, N., Trinder, S. & Lewis, S. E. M. (2000) Spermatozoa DNA-integrity: correlation with fertilization in vitro and pregnancy rates in IVF and ICSI. Human Reproduction 15, abstract book 1, 115.
    • (2000) Human Reproduction , vol.15 , pp. 115
    • Donnelly, E.T.1    McClure, N.2    Trinder, S.3    Lewis, S.E.M.4
  • 10
    • 0026504941 scopus 로고
    • Influence of chromatin structure on the induction of DNA double strand breaks by ionizing radiation
    • Elia, M. C. & Bradley, M. O. (1992) Influence of chromatin structure on the induction of DNA double strand breaks by ionizing radiation. Cancer Research 52, 1580-1586.
    • (1992) Cancer Research , vol.52 , pp. 1580-1586
    • Elia, M.C.1    Bradley, M.O.2
  • 11
    • 0019296108 scopus 로고
    • Relation of mammalian sperm chromatin heterogeneity to fertility
    • Evenson, D. P., Darzynkiewicz, Z. & Melamed, M. R. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210, 1131-1133.
    • (1980) Science , vol.210 , pp. 1131-1133
    • Evenson, D.P.1    Darzynkiewicz, Z.2    Melamed, M.R.3
  • 13
    • 0035665312 scopus 로고    scopus 로고
    • Sperm chromatin structure assay: Its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques
    • Evenson, D. P., Larson, K. L. & Jost, L. K. (2002) Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. Journal of Andrology 23, 25-43.
    • (2002) Journal of Andrology , vol.23 , pp. 25-43
    • Evenson, D.P.1    Larson, K.L.2    Jost, L.K.3
  • 15
    • 0031570793 scopus 로고    scopus 로고
    • Direct visualization of individual DNA molecules by fluorescence microscopy: Characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO
    • Gurrieri, S., Wells, S., Johnson, I. D. & Bustamante, C. (1997) Direct visualization of individual DNA molecules by fluorescence microscopy: characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO. Analytical Biochemistry 249, 44-53.
    • (1997) Analytical Biochemistry , vol.249 , pp. 44-53
    • Gurrieri, S.1    Wells, S.2    Johnson, I.D.3    Bustamante, C.4
  • 16
    • 0035902108 scopus 로고    scopus 로고
    • Genome maintenance mechanisms for preventing cancer
    • Hoeijmakers, J. H. J. (2001) Genome maintenance mechanisms for preventing cancer. Nature 411, 366-374.
    • (2001) Nature , vol.411 , pp. 366-374
    • Hoeijmakers, J.H.J.1
  • 17
    • 0030202411 scopus 로고    scopus 로고
    • A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay
    • Hughes, C. M., Lewis, S. E., McKelvey Martin, V. J. & Thompson, W. (1996) A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Molecular Human Reproduction 2, 613-619.
    • (1996) Molecular Human Reproduction , vol.2 , pp. 613-619
    • Hughes, C.M.1    Lewis, S.E.2    McKelvey Martin, V.J.3    Thompson, W.4
  • 20
    • 0025208418 scopus 로고
    • Stage dependent variation in the radiosensitivity of DNA in developing male germ cells
    • Joshi, D. S., Yick, J., Murray, D. & Meistrich, M. L. (1990) Stage dependent variation in the radiosensitivity of DNA in developing male germ cells. Radiation Research 121, 274-281.
    • (1990) Radiation Research , vol.121 , pp. 274-281
    • Joshi, D.S.1    Yick, J.2    Murray, D.3    Meistrich, M.L.4
  • 21
    • 0037173453 scopus 로고    scopus 로고
    • Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa
    • Kamiguchi, Y. & Tateno, H. (2002) Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa. Mutation Research 504, 183-191.
    • (2002) Mutation Research , vol.504 , pp. 183-191
    • Kamiguchi, Y.1    Tateno, H.2
  • 22
    • 0033871530 scopus 로고    scopus 로고
    • Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques
    • Larson, K. L., DeJonge, C. J., Barnes, A. M., Jost, L. K. & Evenson, D. P. (2000) Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Human Reproduction 15, 1717-1722.
    • (2000) Human Reproduction , vol.15 , pp. 1717-1722
    • Larson, K.L.1    DeJonge, C.J.2    Barnes, A.M.3    Jost, L.K.4    Evenson, D.P.5
  • 23
    • 0031895751 scopus 로고    scopus 로고
    • Sperm deoxyribonucleic acid fragmentation is increased in poor quality semen samples and correlates with failed fertilization in ICSI
    • Lopes, S., Sun, J. G., Juriscova, A., Meriano, J. & Casper, R. F. (1998) Sperm deoxyribonucleic acid fragmentation is increased in poor quality semen samples and correlates with failed fertilization in ICSI. Fertility and Sterility 69, 528-532.
    • (1998) Fertility and Sterility , vol.69 , pp. 528-532
    • Lopes, S.1    Sun, J.G.2    Juriscova, A.3    Meriano, J.4    Casper, R.F.5
  • 24
    • 0030925921 scopus 로고    scopus 로고
    • Two potential clinical applications of the alkaline single-cell gel electrophoresis assay: (1). Human bladder washings and transitional cell carcinoma of the bladder; and (2). Human sperm and male infertility
    • McKelvey Martin, V. J., Melia, N., Walsh, I. K., Johnston, S. R., Hughes, C. M., Lewis, S. E. & Thompson, W. (1997) Two potential clinical applications of the alkaline single-cell gel electrophoresis assay: (1). Human bladder washings and transitional cell carcinoma of the bladder; and (2). Human sperm and male infertility. Mutation Research 375, 93-104.
    • (1997) Mutation Research , vol.375 , pp. 93-104
    • McKelvey Martin, V.J.1    Melia, N.2    Walsh, I.K.3    Johnston, S.R.4    Hughes, C.M.5    Lewis, S.E.6    Thompson, W.7
  • 25
    • 0026724171 scopus 로고
    • Localization of DNase I-hypersensitive regions during rat spermatogenesis: Stage dependent patterns and unique sensitivity of elongating spermatids
    • McPherson, S. M. & Longo, F. J. (1992) Localization of DNase I-hypersensitive regions during rat spermatogenesis: stage dependent patterns and unique sensitivity of elongating spermatids. Molecular Reproduction and Development 31, 268-279.
    • (1992) Molecular Reproduction and Development , vol.31 , pp. 268-279
    • McPherson, S.M.1    Longo, F.J.2
  • 26
    • 0028940712 scopus 로고
    • Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility
    • Manicardi, G. C., Bianchi, P. G., Pantano, S., Azzoni, P., Bianchi, U. & Sakkas, D. (1995) Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biology of Reproduction 52, 864-867.
    • (1995) Biology of Reproduction , vol.52 , pp. 864-867
    • Manicardi, G.C.1    Bianchi, P.G.2    Pantano, S.3    Azzoni, P.4    Bianchi, U.5    Sakkas, D.6
  • 27
    • 0031969435 scopus 로고    scopus 로고
    • DNA strand breaks in ejaculated human spermatozoa: Comparison of susceptibility to the nick translation and terminal transferase assays
    • Manicardi, G. C., Tombacco, A., Bizarro, D., Bianchi, U., Bianchi, P. G. & Sakkas, D. (1998) DNA strand breaks in ejaculated human spermatozoa: comparison of susceptibility to the nick translation and terminal transferase assays. Histochemical Journal 30, 33-39.
    • (1998) Histochemical Journal , vol.30 , pp. 33-39
    • Manicardi, G.C.1    Tombacco, A.2    Bizarro, D.3    Bianchi, U.4    Bianchi, P.G.5    Sakkas, D.6
  • 28
    • 0036108498 scopus 로고    scopus 로고
    • The spectrum of DNA damage in human sperm assessed by single cell electrophoresis (Comet assay) and its relationship to fertilization and embryo development
    • Morris, I. D., Ilott, S., Dixon, L. & Brison, D. R. (2002) The spectrum of DNA damage in human sperm assessed by single cell electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Human Reproduction 17, 990-998.
    • (2002) Human Reproduction , vol.17 , pp. 990-998
    • Morris, I.D.1    Ilott, S.2    Dixon, L.3    Brison, D.R.4
  • 29
    • 0002964507 scopus 로고
    • Semen analysis and sperm washing techniques
    • ed. Gagnon, C., CRC-press, Boca Raton, Fl (USA)
    • Mortimer, D. (1990) Semen analysis and sperm washing techniques. In: Controls of sperm motility: Biological and Clinical Aspects (ed. Gagnon, C.), pp. 263-284. CRC-press, Boca Raton, Fl (USA).
    • (1990) Controls of Sperm Motility: Biological and Clinical Aspects , pp. 263-284
    • Mortimer, D.1
  • 30
    • 0025988295 scopus 로고
    • DNA double-strand breaks measured in individual cells subjected to gel electrophoresis
    • Olive, P. L., Wlodek, D. & Banath, J. P. (1991) DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Research 51, 4671-4676.
    • (1991) Cancer Research , vol.51 , pp. 4671-4676
    • Olive, P.L.1    Wlodek, D.2    Banath, J.P.3
  • 31
    • 0026592534 scopus 로고
    • Factors influencing DNA migration from individual cells subjected to gel electrophoresis
    • Olive, P. L., Wlodek, D., Durand, R. E. & Banath, J. P. (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Experimental Cell Research 198, 259-267.
    • (1992) Experimental Cell Research , vol.198 , pp. 259-267
    • Olive, P.L.1    Wlodek, D.2    Durand, R.E.3    Banath, J.P.4
  • 33
    • 0034814823 scopus 로고    scopus 로고
    • Characterization of subsets of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility
    • Ollero, M., Gil-Guzman, E., Lopez, M. C., Sharma, R. K., Agarwal, A., Larson, K., Evenson, D., Thomas, A. J. & Alvarez, J. G. (2001) Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Human Reproduction 16, 1912-1921.
    • (2001) Human Reproduction , vol.16 , pp. 1912-1921
    • Ollero, M.1    Gil-Guzman, E.2    Lopez, M.C.3    Sharma, R.K.4    Agarwal, A.5    Larson, K.6    Evenson, D.7    Thomas, A.J.8    Alvarez, J.G.9
  • 34
    • 0002087696 scopus 로고
    • Preferential paternal origin of de novo structural chromosome rearrangements
    • AR Liss, New York
    • Olson, S. B. & Magenis, R. E. (1988) Preferential paternal origin of de novo structural chromosome rearrangements. In: The Cytogenetics of Mammalian Autosomal Rearrangements, pp. 583-599. AR Liss, New York.
    • (1988) The Cytogenetics of Mammalian Autosomal Rearrangements , pp. 583-599
    • Olson, S.B.1    Magenis, R.E.2
  • 35
    • 0034064385 scopus 로고    scopus 로고
    • Germ cell suicide: New insights into apoptosis during spermatogenesis
    • Print, C. G. & Lakoski Loveland, K. (2000) Germ cell suicide: new insights into apoptosis during spermatogenesis. BioEssays 22, 423-430.
    • (2000) BioEssays , vol.22 , pp. 423-430
    • Print, C.G.1    Lakoski Loveland, K.2
  • 36
    • 0022345758 scopus 로고
    • Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid
    • Quinn, P., Kerin, J. F. & Warnes, G. M. (1985) Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertility and Sterility 44, 493-498.
    • (1985) Fertility and Sterility , vol.44 , pp. 493-498
    • Quinn, P.1    Kerin, J.F.2    Warnes, G.M.3
  • 37
    • 0034892937 scopus 로고    scopus 로고
    • Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay
    • Ramos, L. & Wetzels, A. M. M. (2001) Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay. Human Reproduction 16, 1703-1707.
    • (2001) Human Reproduction , vol.16 , pp. 1703-1707
    • Ramos, L.1    Wetzels, A.M.M.2
  • 38
    • 0028913310 scopus 로고
    • Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilizing mouse spermatozoa
    • Sakkas, D., Manicardi, G. C., Bianchi, P. G., Bizzaro, D. & Bianchi, U. (1995) Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilizing mouse spermatozoa. Biology of Reproduction 52, 1149-1155.
    • (1995) Biology of Reproduction , vol.52 , pp. 1149-1155
    • Sakkas, D.1    Manicardi, G.C.2    Bianchi, P.G.3    Bizzaro, D.4    Bianchi, U.5
  • 41
    • 0037963687 scopus 로고    scopus 로고
    • Genetic damage in oligospermic patients detected by fluorescence in-situ hybridization, inverse restriction site mutation assay, sperm chromatin structure assay and the Comet assay
    • Schmid, T. E., Kamischke, A., Bollwein, H., Nieschlag, E. & Brinkworth, M. H. (2003) Genetic damage in oligospermic patients detected by fluorescence in-situ hybridization, inverse restriction site mutation assay, sperm chromatin structure assay and the Comet assay. Human Reproduction 18, 1474-1480.
    • (2003) Human Reproduction , vol.18 , pp. 1474-1480
    • Schmid, T.E.1    Kamischke, A.2    Bollwein, H.3    Nieschlag, E.4    Brinkworth, M.H.5
  • 42
    • 0017836734 scopus 로고
    • A study of unscheduled DNA-synthesis induced by X-rays in the germ cells of male mice
    • Sega, G. A., Sotomayor, R. E. & Owens, J. G. (1978) A study of unscheduled DNA-synthesis induced by X-rays in the germ cells of male mice. Mutation Research 49, 239-257.
    • (1978) Mutation Research , vol.49 , pp. 239-257
    • Sega, G.A.1    Sotomayor, R.E.2    Owens, J.G.3
  • 43
    • 0034693836 scopus 로고    scopus 로고
    • Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis
    • Singh, N. P. (2000) Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutation Research 455, 119-135.
    • (2000) Mutation Research , vol.455 , pp. 119-135
    • Singh, N.P.1
  • 44
    • 0031962117 scopus 로고    scopus 로고
    • X-ray induced DNA double-strand breaks in human sperm
    • Singh, N. P. & Stephens, R. E. (1998) X-ray induced DNA double-strand breaks in human sperm. Mutagenesis 13, 75-79.
    • (1998) Mutagenesis , vol.13 , pp. 75-79
    • Singh, N.P.1    Stephens, R.E.2
  • 46
    • 0031045420 scopus 로고    scopus 로고
    • Detection of deoxyribonucleic acid fragmentation in human sperm: Correlation with fertilization in vitro
    • Sun, J., Juriscova, A. & Casper, R. F. (1997) Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biology of Reproduction 56, 602-607.
    • (1997) Biology of Reproduction , vol.56 , pp. 602-607
    • Sun, J.1    Juriscova, A.2    Casper, R.F.3
  • 47
    • 0034788876 scopus 로고    scopus 로고
    • Interrelationships between seminal parameters and sperm nuclear damage before and after density gradient centrifugation: Implications for assisted conception
    • Tomlinson, M. J., Moffatt, O., Manicardi, G. C., Bizarro, D., Afnan, M. & Sakkas, D. (2001) Interrelationships between seminal parameters and sperm nuclear damage before and after density gradient centrifugation: implications for assisted conception. Human Reproduction 16, 2160-2165.
    • (2001) Human Reproduction , vol.16 , pp. 2160-2165
    • Tomlinson, M.J.1    Moffatt, O.2    Manicardi, G.C.3    Bizarro, D.4    Afnan, M.5    Sakkas, D.6
  • 48
    • 0029085833 scopus 로고
    • DNA damage repair in somatic and germ cells in vivo
    • Vogel, E. W. & Natarajan, A. T. (1995) DNA damage repair in somatic and germ cells in vivo. Mutation Research 330, 183-188.
    • (1995) Mutation Research , vol.330 , pp. 183-188
    • Vogel, E.W.1    Natarajan, A.T.2
  • 49
    • 0027178486 scopus 로고
    • Deoxyribonucleic acid loop domain tertiary structure in mammalian spermatozoa
    • Ward, W. S. (1993) Deoxyribonucleic acid loop domain tertiary structure in mammalian spermatozoa. Biology of Reproduction 48, 1193-1201.
    • (1993) Biology of Reproduction , vol.48 , pp. 1193-1201
    • Ward, W.S.1
  • 50
    • 0037182814 scopus 로고    scopus 로고
    • A modified neutral comet assay: Elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody
    • Wojewodzka, M., Buraczewska, I. & Kruszewski, M. (2002) A modified neutral comet assay: elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody. Mutation Research 518, 9-20.
    • (2002) Mutation Research , vol.518 , pp. 9-20
    • Wojewodzka, M.1    Buraczewska, I.2    Kruszewski, M.3


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