Direct visualization of individual DNA molecules by fluorescence microscopy: Characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO

Analytical Biochemistry

Volumn 249, Issue 1, 1997, Pages 44-53

  Gurrieri, Sergio ^a,b,c   Wells, K Sam ^d   Johnson, Iain D ^d   Bustamante, Carlos ^c  

^a UNIVERSITY OF CATANIA   (Italy)

^b CNR   (Italy)

^c UNIVERSITY OF OREGON   (United States)

^d MOLECULAR PROBES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; VISUALIZATION;

ACRIDINE ORANGE; DIRECT VISUALIZATION; DOUBLE STRANDED DNA; ETHIDIUM BROMIDE; FLUORESCENT DYES; HIGH AFFINITY; IMAGING CONDITIONS; SINGLE DNA MOLECULES;

FLUORESCENCE;

1,1' (4,4,7,7 TETRAMETHYL 4,7 DIAZAUNDECAMETHYLENE) BIS 4 [3 METHYL 2,3 DIHYDRO (BENZO 1,3 OXAZOLE) 2 METHYLIDENE] QUINOLINIUM TETRAIODIDE; ACRIDINE ORANGE; BACTERIOPHAGE DNA; CYANINE DYE; DOUBLE STRANDED DNA; ETHIDIUM BROMIDE; FLUORESCENT DYE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; FLUORESCENCE MICROSCOPY; IMAGE QUALITY; NONHUMAN; PRIORITY JOURNAL; SIGNAL DETECTION;

EID: 0031570793     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2102     Document Type: Article

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