Continuous production of (R)-phenylacetylcarbinol in an enzyme-membrane reactor using a potent mutant of pyruvate decarboxylase from Zymomonas mobilis

Biotechnology and Bioengineering

Volumn 74, Issue 4, 2001, Pages 317-325

  Goetz, Günter ^a   Iwan, Peter ^a   Hauer, Bernhard ^b   Breuer, Michael ^a   Pohl, Martina ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b BASF SE   (Germany)

Author keywords

Biotransformation; Carboligation; Kinetic investigation; Pyruvate decarboxylase

Indexed keywords

ALDEHYDES; BIOLOGICAL MEMBRANES; ENZYMES; FERMENTERS; SUBSTRATES; SYNTHESIS (CHEMICAL);

CARBOLIGATION;

BIOTECHNOLOGY;

ACETALDEHYDE; BENZALDEHYDE; EPHEDRINE; METHANOL DERIVATIVE; PHENYLACETYLCARBINOL; PYRUVATE DECARBOXYLASE; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYST; BIOTRANSFORMATION; CELL GROWTH; ENZYME INACTIVATION; ENZYME MECHANISM; MEMBRANE REACTOR; MUTANT; NONHUMAN; PH; REACTION TIME; SACCHAROMYCES CEREVISIAE; ZYMOMONAS MOBILIS;

ACETALDEHYDE; ACETONE; BENZALDEHYDES; BIOMASS; BIOREACTORS; BIOTRANSFORMATION; DECARBOXYLATION; ENZYME STABILITY; EPHEDRINE; FERMENTATION; HYDROGEN-ION CONCENTRATION; INDUSTRIAL MICROBIOLOGY; KINETICS; MEMBRANES, ARTIFICIAL; MICROPORE FILTERS; MUTATION; PYRUVATE DECARBOXYLASE; SACCHAROMYCES CEREVISIAE; ZYMOMONAS;

SACCHAROMYCES CEREVISIAE; ZYMOMONAS MOBILIS;

EID: 0035921177     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1122     Document Type: Article

References (37)

1. Studies on the production of L-acetyl phenyl carbinol by yeast employing benzaldehyde as precursor
2. Study of l-phenylacetylcarbinol
3. Production of phenylacetylcarbinol by various yeast species
4. Reaction engineering for enzymecatalyzed biotransformations
5. Allosteric properties of yeast pyruvate decarboxylase
6. Stereochemistry of the formation of lactaldehyde and acetoin produced by pyruvate decarboxylases of yeast (Saccharomyces sp.) and Z. mobilis: Different distributions between bound forms of the elektrophile, aldehyde, in the two enzymatic reactions
7. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein binding dye
8. Acetoin and phenylacetylcarbinol formation by the pyruvate decarboxylase of Zymomonas mobilis and Saccharomyces carlsbergensis
9. The replacement of Trp392 by alanine influences the decarboxylase/carboligase activity and stability of pyruvate decarboxylase from Zymomonas mobilis
10. Brewer's yeast pyruvate decarboxylase produces acetoin from acetaldehyde: A novel tool to study the mechanism of steps subsequent to carbon dioxide loss
11. A kinetic model for the deactivation of pyruvate decarboxylase (PDC) by benzaldehyde
12. Irreversible inactivation of pyruvate decarboxylase in the presence of substrate and an oxidant
13. Untersuchung über die gewinnung von l-phenylacetylcarbinol
14. Isotopic composition of acetylmethylcarbinol formed by yeast juice
15. Production of phenyl acetyl carbinol by yeast
16. Enzymkinetik
17. Application of alpha-ketoacid decarboxylases in biotransformations
18. Mechanism of the formation of acetoin by yeast and mammalian tissue
19. Biotransformation of benzaldehyde by Saccharomyces cerevisiae: Characterization of the fermentation and toxicity effects of substrates and products
20. Aromatic aldehydes as substrates for yeast and yeast alcohol dehydrogenase
21. Production of L-phenylacetyl carbinol by biotransformation: Product and by-product formation and activities of the key enzymes in wild type and ADH isoenzyme mutants of Saccharomyces cerevisiae
22. Reversible dissociation and unfolding of pyruvate decarboxylase from Zymomonas mobilis
23. Stability investigations on the pyruvate decarboxylase from Zymomonas mobilis
24. Protein design on pyruvate decarboxylase (PDC) by sitedirected mutagenesis
25. Active site mutants of pyruvate decarboxylase from Zymomonas mobilis. A site-directed mutagenesis study of L112, 1472, 1476, E473 and N482
26. Biotransformation for L-ephedrine production
27. The enzyme membrane reactor - An alternative approach for continuous operation with enzymesq
28. Production of L-phenylacetylcarbinol (L-PAC) from benzaldehyde using partially purified pyruvate decarboxylase (PDC)
29. Synthetical potential of thiamin-diphosphate dependent enzymes
30. Production of L-phenylacetylcarbinol by fermentation
31. Some aspects of the phenylacetylcarbinol biosynthesis by Saccharomyces cerevisiae