Ligand binding affinity determined by temperature-dependent circular dichroism: Cyclin-dependent kinase 2 inhibitors

Analytical Biochemistry

Volumn 345, Issue 2, 2005, Pages 187-197

  Mayhood, Todd W ^a   Windsor, William T ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

CDK2; Circular dichroism; Inactive kinase; Inhibitors; Isothermal titration calorimetry; Tm; Temperature; Thermodynamics

Indexed keywords

BINDING ENERGY; CALORIMETERS; CALORIMETRY; CORROSION INHIBITORS; DISSOCIATION; DRUG DELIVERY; ENZYMES; ISOTHERMS; TEMPERATURE; THERMODYNAMICS; THULIUM; TITRATION;

CDK2; CYCLIN-DEPENDENT KINASE; DISSOCIATION CONSTANT; INACTIVE KINASE; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING AFFINITY; THERMODYNAMIC CHARACTERIZATION; THERMODYNAMIC INFORMATION;

DICHROISM;

AG 12275; BMS 250595; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE INHIBITOR; FLAVOPIRIDOL; PROTEIN; PURVALANOL B;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CIRCULAR DICHROISM; CONCENTRATION (PARAMETERS); DEVICE; DISSOCIATION CONSTANT; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; MOLECULE; PREDICTION; PRIORITY JOURNAL; PROTEIN FOLDING; SAMPLING; SOLUBILITY; TEMPERATURE DEPENDENCE; TEMPERATURE DEPENDENT CIRCULAR DICHROISM; THERMODYNAMICS;

EID: 25644434877     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.07.032     Document Type: Article

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