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Alkaline phosphatase was not used after the study with mpa-AuNP-9 because of low solubility. Avidin was used in its place but is not discussed in this comparison.
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76
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25444514555
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Experiments to determine which maa-AuNP gives the most intense stain were not performed.
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note
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Alternative explanations for the smaller mua-AuNP-17 staining of proteins in Figure 8 include (a) particularly effective staining of Neutravidin (relative to other proteins) by mua-AuNP-17 or (b) by particularly ineffective staining of Neutravidin (relative to other proteins) by mpa-AuNP-17.
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Preliminary AFM measurements indicate that the maa-AuNP spot produced by overnight staining of proteins is many layers thick.
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