Lipopolysaccharide Identification with Functionalized Polydiacetylene Liposome Sensors

Journal of the American Chemical Society

Volumn 126, Issue 16, 2004, Pages 5038-5039

  Rangin, Marianne ^a   Basu, Amit ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLENE; LIPOPOLYSACCHARIDE; LIPOSOME;

ARTICLE; BACTERIAL STRAIN; COLORIMETRY; COOLING; LIPID ANALYSIS; MOLECULAR INTERACTION; NONHUMAN; POLYMERIZATION; SENSOR; ULTRASOUND;

ACETYLENE; BIOSENSING TECHNIQUES; COLORIMETRY; GLYCOLIPIDS; GRAM-NEGATIVE BACTERIA; LIPOPOLYSACCHARIDES; LIPOSOMES; POLYACETYLENES; POLYMERS;

EID: 1942489356     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja039822x     Document Type: Article

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21. Control experiments indicate that a fully intact lipopolysaccharide fragment is required to elicit a response. The fingerprint of the intact LPS from E. coli O127:B8 was compared to the responses from the detoxified form of that LPS, which lacks the lipid moieties at the terminus. The CR values from the detoxified form of the LPS were uniformly lower than those from the intact LPS, suggesting that the lipid component of LPS is important for the liposome-LPS interaction. However, the lipid portion alone does not account for a significant component of the response, since exposure of the liposomes to Lipid A does not afford high CR values. Thus, the lipid anchor and the polysaccharide are both required for a colorimetric response to be observed. (See Supporting Information for data.)
22. Data for the blind test is found in the Supporting Information.
23. The presence of the amino acid side chains in 2 or 3 is required for specific interactions with the LPS, as liposomes composed of only 1 exhibit much lower CR values and poor specificity. Additionally, decreasing the net negative charge of the liposomes by the inclusion of a neutral filler lipid increases the CR values while decreasing specificity. These observations suggest that both electrostatic and aromatic carbohydrate interactions play a role in the association of the LPS with the liposomes.