Endothelial KLF2 links local arterial shear stress levels to the expression of vascular tone-regulating genes

American Journal of Pathology

Volumn 167, Issue 2, 2005, Pages 609-618

  Dekker, Rob J ^a   Van Thienen, Johannes V ^a   Rohlena, Jakub ^a   De Jager, Saskia C ^b   Elderkamp, Yvonne W ^a   Seppen, Jurgen ^a   De Vries, Carlie J M ^a   Biessen, Erik A L ^b   Van Berkel, Theo J C ^b   Pannekoek, Hans ^a   Horrevoets, Anton J G ^a,c  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

^c ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ADRENOMEDULLIN; DIPEPTIDYL CARBOXYPEPTIDASE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; ENDOTHELIN 1; MESSENGER RNA; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR KLF2; UNCLASSIFIED DRUG; APOLIPOPROTEIN E; INDUCIBLE NITRIC OXIDE SYNTHASE; KLF2 PROTEIN, HUMAN; KLF2 PROTEIN, MOUSE; KRUPPEL LIKE FACTOR; NITRIC OXIDE SYNTHASE; NOS3 PROTEIN, HUMAN; NOS3 PROTEIN, MOUSE; PEPTIDE; TRANSACTIVATOR PROTEIN;

ADOLESCENT; ADULT; AGED; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; AORTA BIFURCATION; ARTERY BLOOD FLOW; ARTERY INTIMA; ARTERY TONE; ARTICLE; ATHEROSCLEROSIS; BIOMECHANICS; CAROTID ARTERY; CHILD; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; ILIAC ARTERY; IN SITU HYBRIDIZATION; IN VITRO STUDY; IN VIVO STUDY; LASER MICROBEAM MICRODISSECTION; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SHEAR STRESS; TRANSCRIPTION REGULATION; UMBILICAL VEIN; VASCULAR ENDOTHELIUM; ANIMAL; BLOOD VESSEL; CELL CULTURE; CYTOLOGY; DRUG ANTAGONISM; GENE EXPRESSION REGULATION; GENETICS; LASER; MECHANICAL STRESS; METABOLISM; MOUSE MUTANT; PATHOLOGY; PHYSIOLOGY; PULSATILE FLOW;

ADRENOMEDULLIN; ANIMALS; APOLIPOPROTEINS E; BLOOD VESSELS; CAROTID ARTERIES; CELLS, CULTURED; ENDOTHELIN-1; ENDOTHELIUM, VASCULAR; GENE EXPRESSION REGULATION; HUMANS; IN SITU HYBRIDIZATION; KRUPPEL-LIKE TRANSCRIPTION FACTORS; LASERS; MALE; MICE; MICE, KNOCKOUT; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE II; NITRIC OXIDE SYNTHASE TYPE III; PEPTIDES; PULSATILE FLOW; RNA, SMALL INTERFERING; STRESS, MECHANICAL; TRANS-ACTIVATORS;

EID: 25144498154     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0002-9440(10)63002-7     Document Type: Article

References (37)

1. Friedman MH, O'Brien V, Ehrlich LW: Calculations of pulsatile flow through a branch: implications for the hemodynamics of atherogenesis. Circ Res 1975, 36:277-285
2. Topper JN, Gimbrone Jr MA: Blood flow and vascular gene expression: fluid shear stress as a modulator of endothelial phenotype. Mol Med Today 1999, 5:40-46
3. Dewey Jr CF, Bussolari SR, Gimbrone Jr MA, Davies PF: The dynamic response of vascular endothelial cells to fluid shear stress. J Biomech Eng 1981, 103:177-185
4. Topper JN, Cai J, Falb D, Gimbrone Jr MA: Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress. Proc Natl Acad Sci USA 1996, 93:10417-10422
5. Garcia-Cardena G, Comander J, Anderson KR, Blackman BR, Gimbrone Jr MA: Biomechanical activation of vascular endothelium as a determinant of its functional phenotype. Proc Natl Acad Sci USA 2001, 98:4478-4485
6. Dekker RJ, van Soest S, Fontijn RD, Salamanca S, de Groot PG, VanBavel E, Pannekoek H, Horrevoets AJ: Prolonged fluid shear stress induces a distinct set of endothelial cell genes, most specifically lung Kruppel-like factor (KLF2). Blood 2002, 100:1689-1698
7. Wasserman SM, Mehraban F, Komuves LG, Yang RB, Tomlinson JE, Zhang Y, Spriggs F, Topper JN: Gene expression profile of human endothelial cells exposed to sustained fluid shear stress. Physiol Genom 2002, 12:13-23
8. McCormick SM, Frye SR, Eskin SG, Teng CL, Lu CM, Russell CG, Chittur KK, Mclntire LV: Microarray analysis of shear stressed endothelial cells. Biorheology 2003, 40:5-11
9. SenBanerjee S, Lin Z, Atkins GB, Greif DM, Rao RM, Kumar A, Feinberg MW, Chen Z, Simon DI, Luscinskas FW, Michel TM, Gimbrone Jr MA, Garcia-Cardena G, Jain MK: KLF2 is a novel transcriptional regulator of endothelial proinflammatory activation. J Exp Med 2004, 199:1305-1215
10. Davies PF: Flow-mediated endothelial mechanotransduction. Physiol Rev 1995, 75:519-560
11. Drexler H, Hornig B: Endothelial dysfunction in human disease. J Mol Cell Cardiol 1999, 31:51-60
12. Sharefkin JB, Diamond SL, Eskin SG, McIntire LV, Dieffenbach CW: Fluid flow decreases preproendothelin mRNA levels and suppresses endothelin-1 peptide release in cultured human endothelial cells. J Vasc Surg 1991, 14:1-9
13. Rieder MJ, Carmona R, Krieger JE, Pritchard Jr KA, Greene AS: Suppression of angiotensin-converting enzyme expression and activity by shear stress. Circ Res 1997, 80:312-319
14. Shinoki N, Kawasaki T, Minamino N, Okahara K, Ogawa A, Ariyoshi H, Sakon M, Kambayashi J, Kangawa K, Monden M: Shear stress down-regulates gene transcription and production of adrenomedullin in human aortic endothelial cells. J Cell Biochem 1998, 71:109-115
15. Kuo CT, Veselits ML, Barton KP, Lu MM, Clendenin C, Leiden JM: The LKLF transcription factor is required for normal tunica media formation and blood vessel stabilization during murine embryogenesis. Genes Dev 1997, 11:2996-3006
16. Kuo CT, Veselits ML, Leiden JM: LKLF: a transcriptional regulator of single-positive T cell quiescence and survival. Science 1997, 277:1986-1990
17. Banerjee SS, Feinberg MW, Watanabe M, Gray S, Haspel RL, Denkinger DJ, Kawahara R, Hauner H, Jain MK: The Kruppel-like factor KLF2 inhibits peroxisome proliferator-activated receptor-gamma expression and adipogenesis. J Biol Chem 2003, 278:2581-2584
18. Oettgen P: Transcriptional regulation of vascular development. Circ Res 2001, 89:380-388.
19. von der Thüsen JH, van Berkel TJ, Biessen EA: Induction of rapid atherogenesis by perivascular carotid collar placement in apolipoprotein E-deficient and low-density lipoprotein receptor-deficient mice. Circulation 2001, 103:1164-1170
20. Jaffe EA, Nachman RL, Becker CG, Minick CR: Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973, 52:2745-2756
21. Rozen S, Skaletsky HJ: Primers on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Edited by Krawetz S, Misener S. Totowa, Humana Press, 2000, pp 365-386
22. Moorman AF, Houweling AC, de Boer PA, Christoffels VM: Sensitive nonradioactive detection of RNA in tissue sections: novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem 2001, 49:1-8
23. Lennon G, Auffray C, Polymeropoulos M, Soares MB: The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics 1996, 33:151-152
24. Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D: In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 1996, 272:263-267
25. Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L, Trono D: Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol 1998, 72:9873-9880
26. Yuan B, Latek R, Hossbach M, Tuschl T, Lewitter F: siRNA selection server: an automated siRNA oligonucleotide prediction server. Nucl Acids Res 2004, 32:W130-W134
27. Brummelkamp TR, Bernards R, Agami R: A system for stable expression of short interfering RNAs in mammalian cells. Science 2002, 296:550-553
28. Tiscornia G, Singer O, Ikawa M, Verma IM: A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci USA 2003, 100:1844-1848
29. DeRuiter MC, Poelmann RE, VanMunsteren JC, Mironov V, Markwald RR, Gittenberger-de Groot AC: Embryonic endothelial cells transdifferentiate into mesenchymal cells expressing smooth muscle actins in vivo and in vitro. Circ Res 1997, 80:444-451
30. Chandran KB: Flow dynamics in the human aorta. J Biomech Eng 1993, 115:611-616
31. Xu XY, Long Q, Collins MW, Bourne M, Griffith TM: Reconstruction of blood flow patterns in human arteries. Proc Inst Mech Eng 1999, 213:411-421
32. Resnick N, Collins T, Atkinson W, Bonthron DT, Dewey Jr CF, Gimbrone Jr MA: Platelet-derived growth factor-B chain promoter contains a cis-acting fluid shear-stress-responsive element. Proc Natl Acad Sci USA 1993, 90:4591-4595
33. Nagel T, Resnick N, Dewey Jr CF, Gimbrone Jr MA: Vascular endothelial cells respond to spatial gradients in fluid shear stress by enhanced activation of transcription factors. Arterioscler Thromb Vasc Biol 1999, 19:1825-1834
34. Bao X, Lu C, Frangos JA: Temporal gradient in shear but not steady shear stress induces PDGF-A and MCP-1 expression in endothelial cells: role of NO, NF-kB, and egr-1. Arterioscler Thromb Vasc Biol 1999, 19:996-1003
35. Miyakawa AA, de Lourdes Junqueira M, Krieger JE: Identification of two novel shear stress responsive elements in rat angiotensin I converting enzyme promoter. Physiol Genom 2004, 17:107-113
36. Huang PL: Endothelial nitric oxide synthase and endothelial dysfunction. Curr Hypertens Rep 2003, 5:473-480
37. Groenendijk BC, Hierck BP, Gittenberger-De Groot AC, Poelmann RE: Development-related changes in the expression of shear stress responsive genes KLF-2, ET-1, and NOS-3 in the developing cardiovascular system of chicken embryos. Dev Dyn 2004, 230:57-68