Molecular beacon imaging of tumor marker gene expression in pancreatic cancer cells

Cancer Biology and Therapy

Volumn 4, Issue 5, 2005, Pages 561-570

  Yang, Lily ^a   Cao, Zehong ^a   Lin, Yiming ^a   Wood, William C ^a   Staley, Charles A ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

In situ gene expression detection; K ras mutation; Molecular beacon; Molecular imaging; Pancreatic cancer cells; Survivin

Indexed keywords

K RAS PROTEIN; MUTANT PROTEIN; SURVIVIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE; GENE EXPRESSION; GENE MUTATION; HUMAN; HUMAN CELL; IMAGING; MOLECULAR BEACON; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PANCREAS CANCER; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN SYNTHESIS; WILD TYPE;

EID: 25144447660     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.4.5.1670     Document Type: Article

References (36)

1. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000; 100:57-70.
2. Loeb LA. A mutator phenotype in cancer. Cancer Res 2001; 61:3230-9.
3. Kinzler KW, Vogelstein B. Lessons from hereditary colorectal cancer. Cell 1996; 87:159-70.
4. Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 1996; 14:303-8.
5. Tyagi S, Bratu DP, Kramer FR. Multicolor molecular beacons for allele discrimination. Nat Biotechnol 1998; 16:49-53.
6. Bonnet G, Tyagi S, Libchaber A, Kramer FR. Thermodynamic basis of the enhanced specificity of structured DNA probes. Proc Natl Acad Sci USA 1999; 96:6171-6.
7. Frutos AG, Pal S, Quesada M, Lahiri J. Method for detection of single-base mismatches using bimolecular beacons. J Am Chem Soc 2002; 124:2396-7.
8. Broude NE. Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology. Trends Biotechnol 2002; 20:249-56.
9. Fang X, Li JJ, Tan W. Using molecular beacons to probe molecular interactions between lactate dehydrogenase and single-stranded DNA. Anal Chem 2000; 72:3280-5.
10. Heyduk T, Heyduk E. Molecular beacons for detecting DNA binding proteins. Nat Biotechnol 2002; 20:171-6.
11. Zhou W, Galizia G, Lieto E, Goodman SN, Romans KE, Kinzler KW, Vogelstein B, Choti MA, Montgomery EA. Counting alleles reveals a connection between chromosome 18q loss and vascular invasion. Nat Biotechnol 2001; 19:78-81.
12. Sokol DL, Zhang X, Lu P, Gewirtz AM. Real time detection of DNA.RNA hybridization in living cells. Proc Natl Acad Sci USA 1998; 95:11538-43.
13. Perlette J, Tan W. Real-time monitoring of intracellular mRNA hybridization inside single living cells. Anal Chem 2001; 73:5544-50.
14. Fang X, Mi Y, Li JJ, Beck T, Schuster S, Tan W. Molecular beacons: fluorogenic probes for living cell study. Cell Biochem Biophys 2002; 37:71-81.
15. Shah R, El-Deiry WS. p53-Dependent Activation of a Molecular Beacon in Tumor Cells Following Exposure to Doxorubicin Chemotherapy. Cancer Biol Ther 2004; 3:871-5.
16. Hruban RH, van Mansfeld AD, Offerhaus GJ, van Weering DH, Allison DC, Goodman SN, Kensler TW, Bose KK, Cameron JL, Bos JL. K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization. Am J Pathol 1993; 143:545-54.
17. Minamoto T, Mai M, Ronai Z. K-ras mutation: early detection in molecular diagnosis and risk assessment of colorectal, pancreas, and lung cancers - a review. Cancer Detect Prev 2000; 24:1-12.
18. Futakawa N, Kimura W, Yamagata S, Zhao B, Ilsoo H, Inoue T, Sata N, Kawaguchi Y, Kubota Y, Muto T. Significance of K-ras mutation and CEA level in pancreatic juice in the diagnosis of pancreatic cancer. J Hepatobiliary Pancreat Surg 2000; 7:63-71.
19. Deveraux QL, Roy N, Stennicke HR, Van Arsdale T, Zhou Q, Srinivasula SM, Alnemri ES, Salvesen GS, Reed JC. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J 1998; 17:2215-23.
20. LaCasse EC, Baird S, Korneluk RG, MacKenzie AE. The inhibitors of apoptosis (IAPs) and their emerging role in cancer. Oncogene 1998; 17:3247-59.
21. Altieri DC. The molecular basis and potential role of survivin in cancer diagnosis and therapy. Trends Mol Med 2001; 7:542-7.
22. Tanaka K, Iwamoto S, Gon G, Nohara T, Iwamoto M, Tanigawa N. Expression of survivin and its relationship to loss of apoptosis in breast carcinomas. Clin Cancer Res 2000; 6:127-34.
23. Satoh K, Kaneko K, Hirota M, Masamune A, Satoh A, Shimosegawa T. Expression of survivin is correlated with cancer cell apoptosis and is involved in the development of human pancreatic duct cell tumors. Cancer 2001; 92:271-8.
24. Sarela AI, Verbeke CS, Ramsdale J, Davies CL, Markham AF, Guillou PJ. Expression of survivin, a novel inhibitor of apoptosis and cell cycle regulatory protein, in pancreatic adenocarcinoma. Br J Cancer 2002; 86:886-92.
25. Apple SK, Hecht JR, Novak JM, Nieberg RK, Rosenthal DL, Grody WW. Polymerase chain reaction-based K-ras mutation detection of pancreatic adenocarcinoma in routine cytology smears. Am J Clin Pathol 1996; 105:321-6.
26. Berrozpe G, Schaeffer J, Peinado MA, Real FX, Perucho M. Comparative analysis of mutations in the p53 and K-ras genes in pancreatic cancer. Int J Cancer 1994; 58:185-91.
27. Rozenblum E, Schutte M, Goggins M, Hahn SA, Panzer S, Zahurak M, Goodman SN, Sohn TA, Hruban RH, Yeo CJ, Kern SE. Tumor-suppressive pathways in pancreatic carcinoma. Cancer Res 1997; 57:1731-4.
28. Nomoto S, Nakao A, Ando N, Takeda S, Kasai Y, Inoue S, Kaneko T, Takagi H. Clinical application of K-ras oncogene mutations in pancreatic carcinoma: detection of micrometastases. Semin Surg Oncol 1998; 15:40-6.
29. Hruban RH, Sturm PD, Slebos RJ, Wilentz RE, Musler AR, Yeo CJ, Sohn TA, van Velthuysen ML, Offerhaus GJ. Can K-ras codon 12 mutations be used to distinguish benign bile duct proliferations from metastases in the liver? A molecular analysis of 101 liver lesions from 93 patients. Am J Pathol 1997; 151:943-9.
30. Moore PS, Sipos B, Orlandini S, Sorio C, Real FX, Lemoine NR, Gress T, Bassi C, Kloppel G, Kalthoff H, Ungefroren H, Lohr M, Scarpa A. Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K- ras, p53, p16 and DPC4/Smad4. Virchows Arch 2001; 439:798-802.
31. Bonnet G, Krichevsky O, Libchaber A. Kinetics of conformational fluctuations in DNA hairpin-loops. Proc Natl Acad Sci USA 1998; 95:8602-6.
32. Dirks RW, Molenaar C, Tanke HJ. Methods for visualizing RNA processing and transport pathways in living cells. Histochem Cell Biol 2001; 115:3-11.
33. Shibata K, Mori M, Kitano S, Akiyoshi T. Detection of ras gene mutations in peripheral blood of carcinoma patients using CD45 immunomagnetic separation and nested mutant allele specific amplification. Int J Oncol 1998; 12:1333-8.
34. Clayton SJ, Scott FM, Walker J, Callaghan K, Haque K, Liloglou T, Xinarianos G, Shawcross S, Ceuppens P, Field JK, Fox JC. K-ras point mutation detection in lung cancer: comparison of two approaches to somatic mutation detection using ARMS allele-specific amplification. Clin Chem 2000; 46:1929-38.
35. Queneau PE, Adessi GL, Thibault P, Cleau D, Heyd B, Mantion G, Carayon P. Early detection of pancreatic cancer in patients with chronic pancreatitis: diagnostic utility of a K-ras point mutation in the pancreatic juice. Am J Gastroenterol 2001; 96:700-4.
36. Tabata T, Fujimori T, Maeda S, Yamamoto M, Saitoh Y. The role of Ras mutation in pancreatic cancer, precancerous lesions, and chronic pancreatitis. Int J Pancreatol 1993; 14:237-44.