Probing the sweet determinants of brazzein: Wild-type brazzein and a tasteless variant, brazzein-ins(R18a-I18b), exhibit different pH-dependent NMR chemical shifts

Biochemical and Biophysical Research Communications

Volumn 335, Issue 1, 2005, Pages 256-263

  Zhao, Qin ^a   Song, Jikui ^a   Jin, Zheyuan ^b   Danilova, Vicktoria ^b   Hellekant, Göran ^b   Markley, John L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Brazzein; Mutagenesis; NMR spectroscopy; pKa; Sweet taste determinants

Indexed keywords

AMINO ACID DERIVATIVE; ARGININE; ASPARAGINE; BRAZZEIN; CYSTEINE; GLUTAMINE; HISTIDINE; SWEET PROTEIN; TYROSINE; UNCLASSIFIED DRUG;

ALPHA HELIX; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE MUTATION; MUTANT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN VARIANT; PROTON NUCLEAR MAGNETIC RESONANCE; SWEETNESS; WILD TYPE;

HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PLANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; SWEETENING AGENTS; VARIATION (GENETICS);

EID: 25144441794     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.07.049     Document Type: Article

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