Biophysical characterization of the interaction of Limulus polyphemus endotoxin neutralizing protein with lipopolysaccharide

European Journal of Biochemistry

Volumn 271, Issue 10, 2004, Pages 2037-2046

  Andrä, Jörg ^a   Garidel, Patrick ^b   Majerle, Andreja ^c   Jerala, Roman ^c   Ridge, Richard ^d   Paus, Erik ^d   Novitsky, Tom ^d   Koch, Michel H J ^e   Brandenburg, Klaus ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c NATIONAL INSTITUTE OF CHEMISTRY   (Slovenia)

^d Associates of Cape Cod Inc   (United States)

^e EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Endotoxins; IR spectroscopy; LALF; LPS; Tumor necrosis factor

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; CARRIER PROTEIN; DYE; ENDOTOXIN; LIPID A; LIPOPOLYSACCHARIDE; LIPOSOME; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BINDING SITE; BIOASSAY; CELL LYSATE; GEL; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; IMMUNOSTIMULATION; INCUBATION TIME; INTERCALATION COMPLEX; LIMULUS; LIMULUS LYSATE TEST; MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LIPID INTERACTION; SURFACE CHARGE; TEMPERATURE; ZETA POTENTIAL;

ANIMALS; BINDING, COMPETITIVE; CALORIMETRY, DIFFERENTIAL SCANNING; ELECTROCHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEMOCYTES; HORSESHOE CRABS; HUMANS; INVERTEBRATE HORMONES; LEUKOCYTES, MONONUCLEAR; LIMULUS TEST; LIPID A; LIPOPOLYSACCHARIDES; PHASE TRANSITION; PICHIA; POLYMYXIN B; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TUMOR NECROSIS FACTOR-ALPHA; X-RAY DIFFRACTION;

BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA); INVERTEBRATA; LIMULUS; LIMULUS POLYPHEMUS; MEROSTOMATA; POLYPHEMUS;

EID: 2442692637     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04134.x     Document Type: Article

References (50)

1. Rietschel, E.T., Mamat, U., Hamann, L., Wiese, A., Brade, L., Sanchez-Carballo, P., Mattern, T., Zabel, P., Heumann, D., Di Padova, F., Hauschildt, S. & Woltmann, A. (1999) Bacterial endotoxins as inducers of septic shock. Novo Acta Leopoldina 307, 93-122.
2. Seydel, U., Ulmer, A.J., Uhlig, S. & Rietschel, E.Th. (1999) Lipopolysaccharide, a membrane-forming and inflammation-inducing bacterial macromolecule. In Membrane Structure in Disease and Drug Therapy (Zimmer, G., ed.), pp. 217-252. Marcel Dekker Inc., New York.
3. Zähringer, U., Lindner, B. & Rietschel, E.Th. (1999) Chemical structure of lipid A. Recent advances in structural analysis of biologically active molecules. In Endotoxin in Health and Disease (Brade, H., Opal, S.M. Vogel, S.N. & Morrison, D.C., eds), pp. 93-114. Marcel Dekker, New York.
4. Hancock, R.E.W. (1997) Peptide antibiotics. Lancet 349, 418-422.
5. Levy, O. & Elsbach, P. (2001) Bactericidal/permeability-increasing protein in host defense and its efficacy in the treatment of bacterial sepsis. Curr. Infect. Dis. Report 3, 407-412.
6. Aketagawa, J., Miyata, T., Ohtsubo, S., Nakamura, T., Morita, T., Hayashida, H., Iwanaga, S., Takao, T. & Shimonishi, Y. (1986) Primary structure of Limulus anticoagulant anti-lipopolysaccharide factor. J. Biol. Chem. 261, 7357-7365.
7. Alpert, G., Baldwin, G., Thompson, C., Wainwright, N., Novitsky, T.J., Gillis, Z., Parsonnet, J., Fleisher, G.R. & Siber, G.R. (1992) Limulus antilipopolysaccharide factor protects rabbits from meningococcal endotoxin shock. J. Infect. Dis. 165, 494-500.
8. Ridge, R.J., Paus, E.J., Novitsky, T.J. & Ketchum, P.A. (2000) Reversible binding of heparin to the loop peptide of endotoxin neutralizing protein. J. Endotoxin Res. 6, 17-23.
9. Aihara, H., Ito, Y., Kurumizaka, H., Terada, T., Yokoyama, S. & Shibata, T. (1997) An interaction between a specified surface of the C-terminal domain of recA protein and double-stranded DNA for homologous pairing. J. Mol. Biol. 274, 213-228.
10. Kloczewiak, M., Black, K.M., Loiselle, P. & Cavaillon, J.M. (1994) Synthetic peptides that mimic the binding site of horse-shoe crab antilipopolysaccharide factor. J. Infect. Dis. 170, 1490-1497.
11. Morita, T., Ohtsubo, S., Nakamura, T., Tanaka, S., Iwanaga, S., Ohasei, K. & Niwa, M. (1985) Isolation and biological activities of Limulus anticoagulans (anti-LPS factor) which interacts with lipopolysaccharide (LPS). J. Biochem. 97, 1611-1620.
12. Roth, R.I., Su, D., Child, A.H., Wainwright, N.R. & Levin, J. (1998) Limulus antilipopolysaccharide factor prevents mortality late in the course of endotoxemia. J. Infect. Dis. 177, 388-394.
13. Stack, A.M., Saladino, R.A., Siber, G.R., Thompson, C., Marra, M.N., Novitsky, T.J. & Fleisher, G.R. (1997) A comparison of bactericidal/ permeability versus increasing protein variant recombinant endotoxin- neutralizing protein for the treatment of Escherichia coli sepsis in rats. Crit. Care Med. 25, 101-105.
14. Weiner, D.L., Kuppermann, N., Saladino, R.A., Thompson, C.M., Novitsky, T.J., Siber, G.R. & Fleisher, G.R. (1996) Comparison of early and late treatment with a recombinant endotoxin neutralizing protein in a rat model of Escherichia coli sepsis. Crit. Care Med. 24, 1514-1517.
15. Galanos, C., Lüderitz, O. & Westphal, O. (1969) A new method for the extraction of R-lipopolysaccharides. Eur. J. Biochem. 9, 245-249.
16. Paus, E.J., Willey, J., Ridge, R.J., Legg, C.R., Finkelman, M.A., Novitsky, T.J. & Ketchum, P.A. (2002) Production of recombinant endotoxin neutralizing protein in Pichia pastoris and methods for its purification. Protein Expr. Purif. 26, 202-210.
17. Friberger, P., Sörskog, L., Nilsson, K. & Knös, M. (1987) The use of a quantitative assay in endotoxin testing. In Detection of Bacterial Endotoxin with the Limulus Amebocyte Lysate Test (Watson, S.W., Levin, J. & Novitsky, T.J., eds), pp. 149-169. Alan R. Liss, New York.
18. Jürgens, G., Müller, M., Garidel, P., Koch, M.H.J., Nakakubo, H., Blume, A. & Brandenburg, K. (2002) Investigation into the interaction of recombinant human serum albumin with Re-lipopolysaccharide and lipid A. J. Endotoxin Res. 8, 115-126.
19. Brandenburg, K., Jürgens, G., Müller, M., Fukuoka, S. & Koch, M.H.J. (2001) Biophysical characterization of lipopolysaccharide and lipid A inactivation by lactoferrin. Biol. Chem. 382, 1215-1225.
20. Blume, A. & Garidel, P. (1999) Lipid model membranes and biomembranes. In From Macromolecules to Man (Kemp, R.B., ed.), pp. 109-173. Elsevier, Amsterdam.
21. Garidel, P. & Blume, A. (2000) The interaction of alkaline earth cations with the negatively charged phospholipid 1,2-dimyristoyl-sn-glycero-3- phosphoglycerol: a differential scanning and isothermal titration calorimetry study. Langmuir 15, 5526-5534.
22. Horovitz, A. & Levitzky, A. (1987) An accurate method for determination of receptor-ligand and enzyme-inhibitor dissociation constants from displacement curves. Proc. Natl Acad. Sci. USA 84, 6654-6658.
23. Majerle, A., Kidric, J. & Jerala, R. (2003) Enhancement of antibacterial and lipopolysaccharide binding activities of a human lactoferrin peptide fragment by the addition of acyl chain. Antimicrob. Chemother. 51, 1159-1165.
24. Dziarski, R., Tapping, R.I. & Tobias, P.S. (1998) Binding of bacterial peptidoglycan to CD14. J. Biol. Chem. 273, 8680-8690.
25. Koch, M.H.J. & Bordas, J. (1983) X-ray diffraction and scattering on disordered systems using synchrotron radiation. Nucl. Instr. Meth. 208, 461-469.
26. Gabriel, A. (1977) Position-sensitive X-ray detector. Rev. Sci. Instrum. 48, 1303-1305.
27. Boulin, C., Kempf, R., Koch, M.H.J. & McLaughlin, S.M. (1986) Data appraisal, evaluation and display for synchrotron radiation experiments: Hardware and software. Nucl. Instr. Meth. A249, 399-407.
28. Brandenburg, K., Funari, S.S., Koch, M.H.J. & Seydel, U. (1999) Investigation into the acyl chain packing of endotoxins and phospholipids under near physiological conditions by WAXS and FTIR spectroscopy. J. Struct. Biol. 128, 175-186.
29. Gutsmann, T., Schromm, A.B., Koch, M.H.J., Kusumoto, S., Fukase, K., Oikawa, M., Seydel, U. & Brandenburg, K. (2000) Lipopolysaccharide-binding protein-mediated interaction of lipid A from different origin with phospholipid membranes. Phys. Chem. Chem. Phys. 2, 4521-4528.
30. Schromm, A.B., Brandenburg, K., Rietschel, E.Th., Flad, H.-D., Carroll, S.F. & Seydel, U. (1996) Lipopolysaccharide binding protein (LBP) mediates CD14-independent intercalation of lipopolysaccharide into phospholipid membranes. FEBS Lett. 399, 267-271.
31. Kröner, E.E., Peskar, B.A., Fischer, H. & Ferber, E. (1981) Control of arachidonic acid accumulation in bone marrow-derived macrophages by acyltransferases. J. Biol. Chem. 256, 3690-3697.
32. Hoess, A., Watson, S., Siber, G.R. & Liddington, R. (1993) Crystal structure of an endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS factor, at 1.5 Å resolution. EMBO J. 12, 3351-3356.
33. Ivanova, V.P. (2000) Theoretical and experimental study of protein-lipid interactions. PhD Thesis, Georg-August Universität, Göttingen, Sweden.
34. David, S.A., Balasubramanian, K.A., Mathan, V.I. & Balaram, P. (1992) Analysis of the binding of polymyxin B to endotoxic lipid A and core glycolipid using a fluorescent displacement probe. Biochim. Biophys. Acta. 1165, 147-152.
35. Brandenburg, K., Koch, M.H.J. & Seydel, U. (1990) Phase diagram of lipid A from Salmonella minnesota and Escherichia coli rough mutant lipopolysaccharide. J. Struct. Biol. 105, 11-21.
36. Brandenburg, K., Jürgens, G., Andrä, J., Lindner, B., Koch, M.H.J., Blume, A. & Garidel, P. (2002) Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins. Eur. J. Biochem. 269, 5972-5981.
37. Jürgens, G., Müller, M., Koch, M.H.J. & Brandenburg, K. (2001) Interaction of hemoglobin with enterobacterial lipopolysaccharide and lipid A: Physicochemical characterization and biological activity. Eur. J. Biochem. 268, 4233-4242.
38. Brandenburg, K., Koch, M.H.J. & Seydel, U. (1998) Biophysical characterization of lysozyme binding to LPS Re and lipid A. Eur. J. Biochem. 258, 686-695.
39. Gutsmann, T., Müller, M., Carroll, S.F., MacKenzie, R.C., Wiese, A. & Seydel, U. (2001) Dual role of lipopolysaccharide (LPS) -binding protein in neutralization of LPS and enhancement of LPS-induced activation of mononuclear cells. Infect. Immun. 6, 6942-6950.
40. Hailman, E., Lichenstein, H.S., Wurfel, M.M., Miller, D.S., Johnson, D.A., Kelley, M., Busse, L.A., Zukowski, M.M. & Wright, S.D. (1994) Lipopolysaccharide (LPS) -binding protein accelerates the binding of LPS to CD14. J. Exp. Med. 179, 269-277.
41. Wright, S.D., Ramos, R.A., Tobias, P.S., Ulevitch, R.J. & Mathison, J.C. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249, 1431-1433.
42. Schromm, A.B., Brandenburg, K., Loppnow, H., Moran, A.P., Koch, M.H.J., Rietschel, E.Th. & Seydel, U. (2000) Biological activities of lipopolysaccharides are determined by the shape of their lipid A portion. Eur. J. Biochem. 267, 2008-2013.
43. Delude, R.L., Savedra, R., Jr, ZhaoH., Thieringer, R., Yamamoto, S., Fenton, M.J. & Golenbock, D.T. (1995) CD14 enhances cellular responses to endotoxin without imparting ligand-specific recognition. Proc. Natl Acad. Sci. USA 92, 9288-9292.
44. Gegner, J.A., Ulevitch, R.J. & Tobias, P.S. (1995) Lipopolysaccharide (LTS) signal transduction and clearance. Dual roles for LPS binding protein and membrane CD14. J. Biol. Chem. 270, 5320-5325.
45. Tobias, P.S., Soldau, K. & Ulevitch, R.J. (1989) Identification of a lipid A binding site in the acute phase reactant lipopolysaccharide binding protein. J. Biol. Chem. 264, 10867-10871.
46. + channel in transmembrane signaling. J. Immunol. 166, 1009-1015.
47. + channel is involved in LPS signaling. J. Endotoxin Res. 7, 243-247.
48. Buschner, S. (1999) Bestimmung der kritischen Aggregatkonzentration von Lipiden: Implikationen für die biologische Wirksamkeit von Endotoxinen. PhD Thesis, Universität Kiel, Germany.
49. Martin, D.K., Bootcov, M.R., Campbell, T.J., French, P.W. & Breit, S.N. (1995) Human macrophages contain a stretch-sensitive potassium channel that is activated by adherence and cytokines. J. Membr. Biol. 147, 305-315.
50. Jack, R.S., Fan, X., Bernheider, M., Rune, G., Ehlers, M., Weber, A., Kirsch, G., Mente, R., Fürli, B., Freudenberg, M., Schmitz, G., Stelter, F. & Schütt, C. (1997) Lipopolysaccharide-binding protein is required to combat a murine Gram-negative bacterial infection. Nature 38, 742-745.