Production of recombinant endotoxin neutralizing protein in Pichia pastoris and methods for its purification

Protein Expression and Purification

Volumn 26, Issue 2, 2002, Pages 202-210

  Paus, Erik J ^a   Willey, Joanne ^b   Ridge, Richard J ^a   Legg, Charles R ^a   Finkelman, Malcolm A ^a   Novitsky, Thomas J ^a   Ketchum, Paul A ^a  

^a Associates of Cape Cod Inc   (United States)

^b HOFSTRA UNIVERSITY   (United States)

Author keywords

Endotoxin binding protein; LALF; Limulus; Pichia pastoris; Purification; rENP

Indexed keywords

LIMULUS; PICHIA; PICHIA PASTORIS; SYNTHETIC CONSTRUCT;

ANTILIPOPOLYSACCHARIDE FACTOR (LIMULUS); HORMONE; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; FERMENTATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LIMULUS; MOLECULAR CLONING; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOUTHERN BLOTTING;

ANIMALS; BLOTTING, SOUTHERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; HORSESHOE CRABS; INVERTEBRATE HORMONES; PICHIA; PLASMIDS; RECOMBINANT PROTEINS;

EID: 0036426424     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00508-9     Document Type: Article

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