The fluorolysis assay, a highly sensitive method for measuring the cytolytic activity of T cells at very low numbers

Journal of Immunological Methods

Volumn 267, Issue 2, 2002, Pages 99-108

  Kienzle, Norbert ^a,b   Olver, Stuart ^a   Buttigieg, Kathy ^a   Kelso, Anne ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

Author keywords

51Cr release; CD8 T cell; Cytolysis; EGFP; Ex vivo; FACS

Indexed keywords

ANTIBODY; CD3 ANTIGEN; CD8 ANTIGEN; CHROMIUM 51; FLUOROCHROME; GREEN FLUORESCENT PROTEIN; PEPTIDE;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN SPECIFICITY; ARTICLE; CELL SPECIFICITY; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOLYSIS; FEMALE; FLOW CYTOMETRY; FLUOROLYSIS ASSAY; GENETIC TRANSFECTION; IMMUNOLOGICAL PROCEDURES; INCUBATION TIME; INTERMETHOD COMPARISON; LYMPHOCYTE COUNT; MOUSE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; T LYMPHOCYTE; TARGET CELL;

ANIMALS; CELL LINE; CHROMIUM RADIOISOTOPES; CYTOTOXICITY TESTS, IMMUNOLOGIC; FEMALE; FLOW CYTOMETRY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICE; MICE, INBRED C57BL; MICE, INBRED DBA; PROPIDIUM; T-LYMPHOCYTES, CYTOTOXIC; TRANSFECTION;

EID: 0037105972     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00150-3     Document Type: Article

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