Staphylococcus aureus sortase transpeptidase SrtA: Insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism

Biochemistry

Volumn 44, Issue 33, 2005, Pages 11188-11200

  Frankel, Brenda A ^a   Kruger, Ryan G ^a   Robinson, Dana E ^b   Kelleher, Neil L ^b   McCafferty, Dewey G ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; HYDROLYSIS; IONIZATION; ISOTOPES; MASS SPECTROMETRY; PH EFFECTS; PHYSIOLOGY; PROTONS;

ACTIVE SITE; CELL WALL; ELECTROSPRAY MASS SPECTROMETRY; GRAM-POSITIVE BACTERIA;

ENZYMES;

BACTERIAL ENZYME; CYSTEINE; IODOACETAMIDE; PEPTIDOGLYCAN; RECOMBINANT ENZYME; SOLVENT; SORTASE; SORTASE TRANSPEPTIDASE SRTA; SORTASE TRANSPEPTIDASE SRTB; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS ANTHRACIS; BACTERIAL CELL WALL; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GRAM POSITIVE BACTERIUM; HYDROLYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTON TRANSPORT; STAPHYLOCOCCUS AUREUS; STEADY STATE;

AMINOACYLTRANSFERASES; BACILLUS ANTHRACIS; BACTERIAL PROTEINS; CATALYSIS; CELL WALL; CYSTEINE; CYSTEINE ENDOPEPTIDASES; ENZYME ACTIVATION; HYDROGEN-ION CONCENTRATION; KINETICS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; STAPHYLOCOCCUS AUREUS;

BACILLUS ANTHRACIS; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 23944522769     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi050141j     Document Type: Article

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