Probing the mechanism of hamster arylamine N-acetyltransferase 2 acetylation by active site modification, site-directed mutagenesis, and pre-steady state and steady state kinetic studies

Biochemistry

Volumn 43, Issue 25, 2004, Pages 8234-8246

  Wang, Haiqing ^a   Vath, Gregory M ^a   Gleason, Kara J ^a   Hanna, Patrick E ^a   Wagner, Carston R ^a,b  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLATION; ALKYLATION; CATALYSIS; COENZYMES; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SENSORS; ISOTOPES; PH EFFECTS; RATE CONSTANTS; SOLVENTS;

ACETYL COENZYMES; PROKARYOTES; PROTEASES; SECOND-ORDER RATE CONSTANTS;

BIOCHEMISTRY;

ACETAMIDE DERIVATIVE; AROMATIC AMINE; ARYLAMINE ACETYLTRANSFERASE; ASPARTIC ACID; BROMOACETAMIDE; BROMOACETIC ACID; CYSTEINE PROTEINASE; HISTIDINE; HYDRAZINE DERIVATIVE; IODOACETAMIDE; PROTEINASE; UNCLASSIFIED DRUG;

ACETYLATION; ALKYLATION; ANIMAL CELL; ARTICLE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME MODIFICATION; FRACTIONATION; HYDROPHOBICITY; NONHUMAN; PH MEASUREMENT; PRIORITY JOURNAL; PROKARYOTE; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STEADY STATE; STRUCTURE ACTIVITY RELATION; SUBSTITUTION REACTION; THREE DIMENSIONAL IMAGING;

ACETAMIDES; ACETYL COENZYME A; ACETYLATION; ALKYLATING AGENTS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARYLAMINE N-ACETYLTRANSFERASE; BINDING SITES; CRICETINAE; CYSTEINE; DEUTERIUM; ENZYME INHIBITORS; HYDROGEN-ION CONCENTRATION; IODOACETAMIDE; ISOENZYMES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NITROPHENOLS; PAPAIN; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

ANIMALIA; CRICETINAE; PROKARYOTA;

EID: 3042513718     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0497244     Document Type: Article

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