A high-throughput fluorescence polarization assay specific to the CD4 binding site of HIV-1 glycoproteins based on a fluorescein-labelled CD4 mimic

Biochemical Journal

Volumn 390, Issue 1, 2005, Pages 29-39

  Stricher, François ^a   Martin, Loïc ^a   Barthe, Philippe ^b   Pogenberg, Vivian ^b   Mechulam, Alain ^c   Menez, André ^a   Roumestand, Christian ^b   Veas, Francisco ^c   Royer, Catherine ^b   Vita, Claudio ^a  

^a CEA SACLAY   (France)

^b UNIV MONTPELLIER   (France)

^c INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

Author keywords

CD4 binding; Drug discovery; Fluorescence polarization; HIV inhibitors; Protein protein interaction; Three dimensional structure

Indexed keywords

ANTIGENS; CELLS; NUCLEAR MAGNETIC RESONANCE; POLARIZATION;

INHIBITORS; THREE-DIMENSIONAL STRUCTURE;

FLUORESCENCE;

4 BENZOYL 1 [(4 METHOXY 1H PYRROLO[2,3 B]PYRIDIN 3 YL)OXOACETYL] 2 METHYLPIPERAZINE; ANTIVIRUS AGENT; CD4 ANTIGEN; FLUORESCEIN; GLYCOPROTEIN GP 120; UNCLASSIFIED DRUG;

ANISOTROPY; ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; DISSOCIATION CONSTANT; DRUG MECHANISM; DRUG SYNTHESIS; DRUG TARGETING; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; PROTON NUCLEAR MAGNETIC RESONANCE; THREE DIMENSIONAL IMAGING;

ANTIGENS, CD4; DRUG DELIVERY SYSTEMS; FLUORESCENCE POLARIZATION IMMUNOASSAY; HIV ENVELOPE PROTEIN GP120; HIV-1; MOLECULAR MIMICRY; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 23944451595     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041953     Document Type: Article

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