Importin α associates with membranes and participates in nuclear envelope assembly in vitro

EMBO Journal

Volumn 23, Issue 7, 2004, Pages 1526-1535

  Hachet, Virginie ^a   Köcher, Thomas ^a   Wilm, Matthias ^a   Mattaj, Iain W ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Importin ; Nuclear assembly; Nuclear envelope; Protein phosphorylation

Indexed keywords

ADAPTOR PROTEIN; KARYOPHERIN ALPHA; KARYOPHERIN BETA; MUTANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; CONTROLLED STUDY; EGG; IN VITRO STUDY; MEMBRANE BINDING; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; XENOPUS;

ACTIVE TRANSPORT, CELL NUCLEUS; ALPHA KARYOPHERINS; AMINO ACID SEQUENCE; ANIMALS; BETA KARYOPHERINS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEAR ENVELOPE; NUCLEAR LOCALIZATION SIGNALS; NUCLEAR PORE COMPLEX PROTEINS; OOCYTES; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; XENOPUS LAEVIS;

ANIMALIA;

EID: 2342478595     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.emboj.7600154     Document Type: Article

References (33)

1. Askjaer P, Galy V, Hannak E, Mattaj IW (2002) Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos. Mol Biol Cell 13: 4355-4370
2. Azuma Y, Tabb MM, Vu L, Nomura M (1995) Isolation of a yeast protein kinase that is activated by the protein encoded by SRP1 (Srp1p) and phosphorylates Srp1p complexed with nuclear localization signal peptides. Proc Natl Acad Sci USA 92: 5159-5163
3. Azuma Y, Takio K, Tabb MM, Vu L, Nomura M (1997) Phosphorylation of Srp1p, the yeast nuclear localization signal receptor, in vitro and in vivo. Biochimie 79: 247-259
4. Bamba C, Bobinnec Y, Fukuda M, Nishida E (2002) The GTPase Ran regulates chromosome positioning and nuclear envelope assembly in vivo. Curr Biol 12: 503-507
5. Ellenberg J, Siggia ED, Moreira JE, Smith CL, Presley JF, Worman HJ, Lippincott-Schwartz J (1997) Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis. J Cell Biol 138: 1193-1206
6. Foisner R (2001) Inner nuclear membrane proteins and the nuclear lamina. J Cell Sci 114: 3791-3792
7. Geles KG, Johnson JJ, Jong S, Adam SA (2002) A role for Caenorhabditis elegans importin IMA-2 in germ line and embryonic mitosis. Mol Biol Cell 13: 3138-3147
8. Gorjanacz M, Adam G, Torok I, Mechler BM, Szlanka T, Kiss I (2002) Importin-alpha 2 is critically required for the assembly of ring canals during Drosophila oogenesis. Dev Biol 251: 271-282
9. Görlich D, Prehn S, Laskey RA, Hartmann E (1994) Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79: 767-778
10. Görlich D, Kutay U (1999) Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol 15: 607-660
11. Gruss OJ, Carazo-Salas RE, Schatz CA, Guarguaglini G, Kast J, Wilm M, Le Bot N, Vernos I, Karsenti E, Mattaj IW (2001) Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity. Cell 104: 83-93
12. Harel A, Chan RC, Lachish-Zalait A, Zimmerman E, Elbaum M, Forbes DJ (2003) Importin β negatively regulates nuclear membrane fusion and nuclear pore complex assembly. Mol Biol Cell 14:4387-4396
13. Hartinger J, Stenius K, Hogemann D, Jahn R (1996) 16-BAC/SDS-PAGE: a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal Biochem 240: 126-133
14. Hetzer M, Bilbao-Cortes D, Walther TC, Gruss OJ, Mattaj IW (2000) GTP hydrolysis by Ran is required for nuclear envelope assembly. Mol Cell 5: 1013-1024
15. Hetzer M, Gruss OJ, Mattaj IW (2002) The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly. Nat Cell Biol 4: 177-184
16. Hetzer M, Meyer HH, Walther TC, Bilbao-Cortes D, Warren G, Mattaj IW (2001) Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Nat Cell Biol 3: 1086-1091
17. Holmer L, Worman HJ (2001) Inner nuclear membrane proteins: functions and targeting. Cell Mol Life Sci 58: 1741-1747
18. Jäkel S, Mingot JM, Schwarzmaier P, Hartmann E, Görlich D (2002) Importins fulfil a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains. EMBO J 21: 377-386
19. Köcher T, Allmaier G, Wilm M (2003) Nanoelectrospray-based detection and sequencing of substoichiometric amounts of phosphopeptides in complex mixtures. J Mass Spectrom 38: 131-137
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
21. Marshall ICB, Wilson KL (1997) Nuclear envelope assembly after mitosis. Trends Cell Biol 7: 69-74
22. Matsusaka T, Imamoto N, Yoneda Y, Yanagida M (1998) Mutations in fission yeast CutI5, an importin alpha homolog, lead to mitotic progression without chromosome condensation. Curr Biol 8: 1031-1034
23. Mattaj IW (2004) Sorting out the nuclear envelope from the endoplasmic reticulum. Nat Rev Mol Cell Biol 5: 65-69
24. Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K (2001) Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. Cell 104: 95-106
25. Quimby BB, Dasso M (2003) The small GTPase Ran: interpreting the signs. Curr Opin Cell Biol 15: 338-344
26. Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68: 850-858
27. Timinszky G, Tirian L, Nagy FT, Toth G, Perczel A, Kiss-Laszlo Z, Boros I, Clarke PR, Szabad J (2002) The importin-beta P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope. J Cell Sci 115: 1675-1687
28. Tirian L, Timinszky G, Szabad J (2003) P446L-importin-beta inhibits nuclear envelope assembly by sequestering nuclear envelope assembly factors to the microtubules. Eur J Cell Biol 82: 351-359
29. Walther TC, Askjaer P, Gentzel M, Habermann A, Griffiths G, Wilm M, Mattaj IW, Hetzer M (2003) RanGTP mediates nuclear pore complex assembly. Nature 42: 4689-4694
30. Wiese C, Goldberg MW, Allen TD, Wilson KL (1997) Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport-dependent 'envelope smoothing' event. J Cell Sci 110: 1489-1502
31. Yang L, Guan T, Gerace L (1997) Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis. J Cell Biol 137: 1199-1210
32. Zhang C, Clarke PR (2000) Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts. Science 288: 1429-1432
33. Zhang C, Hutchins JR, Muhlhausser P, Kutay U, Clarke PR (2002) Role of importin β in the control of nuclear envelope assembly by Ran. Curr Biol 12: 498-502