Developmental biology: Stem cell depletion through epidermal deletion of Rac1

Science

Volumn 309, Issue 5736, 2005, Pages 933-935

  Benitah, Salvador Aznar ^a   Frye, Michaela ^a   Glogauer, Michael ^b   Watt, Fiona M ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; BIOLOGY; ENZYMES; GENES;

CELL ADHESION; DEVELOPMENTAL BIOLOGY; MAMMALIAN EPIDERMIS; STEM CELLS;

CELLS;

MESSENGER RNA; MYC PROTEIN; P21 ACTIVATED KINASE 2; PHOSPHOTRANSFERASE; PROTEIN P21; RAC PROTEIN; RAC1 PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG; 4 HYDROXYTAMOXIFEN; 4-HYDROXYTAMOXIFEN; DIFFERENTIATION ANTIGEN; DRUG DERIVATIVE; MYC PROTEIN, HUMAN; MYC PROTEIN, MOUSE; NEUROPEPTIDE; PROTEIN SERINE THREONINE KINASE; RAC1 PROTEIN, MOUSE; TAMOXIFEN;

DEVELOPMENTAL BIOLOGY;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL DIVISION; CELL POPULATION; CELL PROLIFERATION; CELL RENEWAL; CELL STIMULATION; CONTROLLED STUDY; CYTOSKELETON; DEPLETION; ENZYME PHOSPHORYLATION; EPIDERMIS; FLOW CYTOMETRY; GENE DELETION; GENE EXPRESSION; HAIR FOLLICLE; HUMAN; HUMAN CELL; KERATINOCYTE; MOUSE; MOUSE STRAIN; NONHUMAN; PLEIOTROPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; REGULATORY MECHANISM; RNA INTERFERENCE; SEBACEOUS GLAND; STEM CELL; ANIMAL; APOPTOSIS; C57BL MOUSE; CELL CULTURE; CYTOLOGY; GENETICS; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; SKIN TUMOR; SQUAMOUS CELL CARCINOMA; TRANSGENIC MOUSE;

ANIMALIA; MAMMALIA;

ANIMALS; ANTIGENS, DIFFERENTIATION; APOPTOSIS; CARCINOMA, SQUAMOUS CELL; CELL DIFFERENTIATION; CELLS, CULTURED; EPIDERMIS; GENE DELETION; HUMANS; KERATINOCYTES; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; NEUROPEPTIDES; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS C-MYC; RAC GTP-BINDING PROTEINS; SKIN NEOPLASMS; STEM CELLS; TAMOXIFEN;

EID: 23244452354     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1113579     Document Type: Article

References (22)

1. D. M. Owens, F. M. Watt, Nat. Rev. Cancer 3, 444 (2003).
2. F. M. Watt, EMBO J. 21, 3919 (2002).
3. I. Arnold, F. M. Watt, Curr. Biol. 11, 558 (2001).
4. R. L. Waikel, Y. Kawachi, P. A. Waikel, X. J. Wang, D. R. Roop, Nat. Genet 28, 165 (2001).
5. M. J. Murphy, A. Wilson, A. Trumpp, Trends Cell Biol. 15, 128 (2005).
6. M. Frye, C. Gardner, E. R. Li, I. Arnold, F. M. Watt, Development 130, 2793 (2003).
7. S. Etienne-Manneville, A. Hall, Nature 420, 629 (2002).
8. S. A. Benitah, P. F. Valeron, L. van Aelst, C. J. Marshall, J. C. Lacal, Biochim. Biophys. Acta 1705, 121 (2004).
9. M. Glogauer et al., J. Immunol. 170, 5652 (2003).
10. K. U. Hong, S. D. Reynolds, S. Watkins, E. Fuchs, B. R. Stripp, Am. J. Pathol. 164, 577 (2004).
11. T. Tumbar et al., Science 303, 359 (2004).
12. R. J. Morris et al., Nat. Biotechnol. 22, 411 (2004).
13. S. Aznar Benitah, F. M. Watt, unpublished data.
14. J. Legg, U. B. Jensen, S. Broad, I. Leigh, F. M. Watt, Development 130, 6049 (2003).
15. K. M. Braun et al., Development 130, 5241 (2003).
16. R. Morris, C. S. Potten, J. Invest. Dermatol. 112, 470 (1999).
17. Z. Huang, J. A. Traugh, J. M. Bishop, Mol. Cell. Biol. 24, 1582 (2004).
18. A. Gandarillas, F. M. Watt, Genes Dev. 11, 2869 (1997).
19. Y. Gu et al., Science 302, 445 (2003).
20. M. J. Walmsley et al., Science 302, 459 (2003).
21. T. S. Stappenbeck, K. I. Gordon, Development 127, 2629 (2000).
22. This work was funded by Cancer Research UK, by the European Union (EU) EuroStemCell network, and by European Molecular Biology Organization and EU Marie Curie fellowships (S.A.B.). We thank everyone who provided advice and technical support.