Hematopoietic Cell Regulation by Rac1 and Rac2 Guanosine Triphosphatases

Science

Volumn 302, Issue 5644, 2003, Pages 445-449

  Gu, Yi ^a   Filippi, Marie Dominique ^a   Cancelas, Jose A ^a,b   Siefring, Jamie E ^a   Williams, Emily P ^a   Jasti, Aparna C ^a   Harris, Chad E ^a   Lee, Andrew W ^a   Prabhakar, Rethinasamy ^a   Atkinson, Simon J ^c   Kwiatkowski, David J ^d   Williams, David A ^a  

^a CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^c INDIANA UNIVERSITY   (United States)

^d BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; ENZYMES;

BONE MARROW;

CELLS;

ACTIN; GUANOSINE TRIPHOSPHATASE; RAC1 PROTEIN; RAC2 PROTEIN; SUPEROXIDE; UNCLASSIFIED DRUG;

ALLELE; ANIMAL CELL; ARTICLE; BONE MARROW; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; GENE DELETION; HEMATOPOIETIC CELL; LEUKOCYTE MIGRATION; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; STEM CELL;

ACTINS; ANIMALS; APOPTOSIS; BONE MARROW TRANSPLANTATION; CELL ADHESION; CELL CYCLE; CELL MOVEMENT; CELL SIZE; COLONY-FORMING UNITS ASSAY; CYCLIN D1; FIBRONECTINS; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL MOBILIZATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; MICE; MICE, INBRED NOD; MICE, SCID; MITOGEN-ACTIVATED PROTEIN KINASES; NEUTROPHILS; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; RAC GTP-BINDING PROTEINS; RAC1 GTP-BINDING PROTEIN; RECOMBINATION, GENETIC; SIGNAL TRANSDUCTION; STEM CELL FACTOR; SUPEROXIDES;

ANIMALIA; MAMMALIA; MURINAE;

EID: 0142084736     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1088485     Document Type: Article

References (32)

1. A. Hall, Science 279, 509 (1998).
2. J. Moll, G. Sansig E. Fattori, H. van der Putten, Oncogene 6, 863 (1991).
3. N. V. Shirsat, R. J. Pignolo, B. L. Kreider, G. Rovera, Oncogene 5, 769 (1990).
4. L. Haataja, J. Groffen, N. Heisterkamp, J. Biol. Chem. 272, 20384 (1997).
5. A. W. Roberts et al., Immunity 10, 183 (1999).
6. F. C. Yang et al., Proc. Natl. Acad. Sci. U.S.A. 98, 5614 (2001).
7. F. C. Yang et al., Immunity 12, 557 (2000).
8. B. Li et al., Science 288, 2219 (2000).
9. B. A. Croker et al., J. Immunol. 168, 3376 (2002).
10. Y. Gu et al., Mol. Cell Biol. 22, 764S (2002).
11. A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, A. Hall, Cell 70, 401 (1992).
12. C. D. Nobes, A. Hall, Cell 81, 53 (1995).
13. L. Van Aelst, C. D'Souza-Schorey, Genes Dev. 11, 2295 (1997).
14. S. Etienne-Manneville, A. Hall, Nature 420, 629 (2002).
15. K. Sugihara et al., Oncogene 17, 3427 (1998).
16. Materials and methods are available as supporting material on Science Online.
17. Rac3 was expressed by reverse transcriptase PCR in both primitive and differentiated hematopoietic cells.
18. R. Palacios, C. Bucana, X. Xie, Proc. Natl. Acad. Sci. U.S.A. 93, 5247 (1996).
19. T. M. Dexter, T. D. Allen, L. G. Lajtha, J. Cell. Physiol. 91, 335 (1976).
20. -/-, 108 ± 9.
21. A. Peled et al., Science 283, 845 (1999).
22. -/- HSC/Ps. Indeed, in the absence of both GTPases, no obvious F-actin was seen on immunofluorescence images, and the cytoplasm of these cells appeared to collapse toward the cell nucleus.
23. -/- neutrophils, the F-actin structure appeared to collapse into a dense network immediately surrounding the nucleus, showing a complete loss of structural organization consistent with a lack of cell spreading (Fig. 4B). Whereas previous reports demonstrate an important role for Rac1 in F-actin assembly in many cells, most of these studies were done in nonhematopoietic cells, which do not express Rac2.
24. M. Glogauer et al., J. Immunol. 170, 5652 (2003).
25. A. Abo et al., Nature 353, 668 (1991).
26. U. G. Knaus, P. G. Heyworth, T. Evans, J. T. Curnutte, G. M. Bokoch, Science 254, 1512 (1991).
27. T. Papayannopoulou, C. Craddock, Acta Haematol. 97, 97 (1997).
28. D. Michaelson et al., J. Cell Biol. 152, 111 (2001).
29. J. C. van der Loo et al., J. Clin. Invest. 102, 1051 (1998).
30. H. Gu, J. D. Marth, P. C. Orban, H. Mossmann, K. Rajewsky, Science 265, 103 (1994).
31. Openlab software, Improvision, Andrew Waterfall, Lexington, MA.
32. We thank J. Towe for technical assistance, Y. Zheng for reviewing the manuscript, M. Hodgson and K. Steward for administrative assistance, D. Marmer and the Flow Core at Cincinnati Children's Hospital Medical Center for assistance with cell sorting, and Amgen and Takara Bio for reagents. Supported by NIH DK627S7 (D.A.W.).