Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein α-subunit

Journal of Biomolecular NMR

Volumn 32, Issue 1, 2005, Pages 31-40

  Abdulaev, Najmoutin G ^a,b   Zhang, Cheng ^c   Dinh, Andy ^c   Ngo, Tony ^a,b   Bryan, Philip N ^a,b   Brabazon, Danielle M ^d   Marino, John P ^a,b   Ridge, Kevin D ^c  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^d LOYOLA UNIVERSITY MARYLAND   (United States)

Author keywords

Eukaryotic protein; G protein; High resolution; Stable isotope labeling; Subtilisin; Transducin

Indexed keywords

ALUMINUM FLUORIDE; BACTERIAL PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; HYBRID PROTEIN; ISOTOPE; SUBTILISIN;

ARTICLE; CHIMERA; ESCHERICHIA COLI; ISOTOPE LABELING; MOLECULAR PROBE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROKARYOTE; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GUANOSINE DIPHOSPHATE; ISOTOPE LABELING; MAGNESIUM; MODELS, MOLECULAR; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; SUBTILISINS;

EUKARYOTA; PROKARYOTA;

EID: 23144435365     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10858-005-3340-5     Document Type: Article

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