Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: Use of a photoactivatable reagent

Proceedings of the National Academy of Sciences of the United States of America

Volumn 98, Issue 9, 2001, Pages 4877-4882

  Cai, Kewen ^a,b   Itoh, Yoshiki ^a,c   Khorana, H Gobind ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b BIOGEN   (United States)

^c TAIHO PHARMACEUTICAL CO LTD   (Japan)

Author keywords

Avidinbiotin affinity; Cysteine mutants; Cytoplasmic domain; Disulfide exchange

Indexed keywords

BIOCYTIN; CROSS LINKING REAGENT; MALEIMIDE DERIVATIVE; N [(2 PYRIDYLDITHIO)ETHYL] 4 AZIDOSALICYLAMIDE; N ETHYLMALEIMIDE; RHODOPSIN; TRANSDUCIN; UNCLASSIFIED DRUG;

ARTICLE; BRAIN MAPPING; COVALENT BOND; PHOTOACTIVATION; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN SECONDARY STRUCTURE; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; AZIDES; BINDING SITES; CATTLE; COS CELLS; CROSS-LINKING REAGENTS; CYSTEINE; DISULFIDES; DITHIOTHREITOL; ETHYLMALEIMIDE; GUANOSINE DIPHOSPHATE; LIGHT; LYSINE; MALEIMIDES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PHOTOLYSIS; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PYRIDINES; RHODOPSIN; SEPHAROSE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSDUCIN; TRYPSIN; ULTRAVIOLET RAYS;

EID: 0035942238     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.051632898     Document Type: Article

References (36)