Improved stability and yield of Fv targeted superantigen by introducing both linker and disulfide bond into the targeting moiety

Biochimie

Volumn 87, Issue 8, 2005, Pages 661-667

  Hao, Huai Jie ^a,b   Jiang, Yong Qiang ^a   Zheng, Yu Ling ^a   Ma, Ru ^a   Yu, Duo Wei ^b  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b NANJING NORMAL UNIVERSITY   (China)

Author keywords

SAg; SEA(D227A); Single chain disulfide stabilized Fv; Stability; Yield

Indexed keywords

3 (4,5 DIMETHYLTHIAZOL 2 YL) 5 (3 CARBOXYMETHOXYPHENYL) 2 (4 SULFOPHENYL) 2H TETRAZOLIUM; CELL EXTRACT; DISULFIDE; GLYCYLGLYCYLGLYCYLSERYLGLYCYLGLYCYLSERINE; IMMUNOTOXIN; PEPTIDE DERIVATIVE; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; STAPHYLOCOCCUS ENTEROTOXIN A; SUPERANTIGEN; TETRAZOLIUM; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CANCER CELL CULTURE; CANCER IMMUNOTHERAPY; CELL INCLUSION; CONTROLLED STUDY; COVALENT BOND; CYTOTOXICITY; DRUG DETERMINATION; DRUG PURIFICATION; DRUG STABILITY; DRUG TARGETING; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE FUSION; GENETIC ENGINEERING; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; LYMPHOCYTE COUNT; NONHUMAN; NONVIRAL GENE THERAPY; QUANTUM YIELD; T LYMPHOCYTE ACTIVATION;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; CARCINOMA, HEPATOCELLULAR; CYTOTOXICITY, IMMUNOLOGIC; DISULFIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG STABILITY; ENTEROTOXINS; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOTOXINS; MUTATION; RECOMBINANT PROTEINS; SUPERANTIGENS; T-LYMPHOCYTES; TUMOR CELLS, CULTURED;

ESCHERICHIA COLI; STAPHYLOCOCCUS;

EID: 23044488673     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biochi.2005.04.005     Document Type: Article

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