High constitutive activity of a virus-encoded seven transmembrane receptor in the absence of the conserved DRY motif (Asp-Arg-Tyr) in transmembrane helix 3

Molecular Pharmacology

Volumn 68, Issue 1, 2005, Pages 11-19

  Rosenkilde, Mette M ^a   Kledal, Thomas N ^b   Schwartz, Thue W ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b HVIDOVRE HOSPITAL   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASPARTIC ACID; CHEMOKINE; CYCLIC AMP; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; FORSKOLIN; GAMMA INTERFERON INDUCIBLE PROTEIN 6; GUANINE NUCLEOTIDE BINDING PROTEIN; INOSITOL PHOSPHATE; MEMBRANE RECEPTOR; PERTUSSIS TOXIN; PROTEIN SUBUNIT; RHODOPSIN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; DNA RESPONSIVE ELEMENT; ENZYME LINKED IMMUNOSORBENT ASSAY; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MOTIF; RECEPTOR BINDING; SUBSTITUTION REACTION; TRANSCRIPTION INITIATION; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ARGININE; ASPARTIC ACID; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; CONSERVED SEQUENCE; COS CELLS; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, CHEMOKINE; RECEPTORS, G-PROTEIN-COUPLED; RHADINOVIRUS; TYROSINE;

EID: 22344450701     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.105.011239     Document Type: Article

References (40)

1. Acharya S and Karnik SS (1996) Modulation of GDP release from transducin by the conserved Glu134-Arg135 sequence in rhodopsin. J Biol Chem 271:25406-25411.
2. Ahuja SK and Murphy PM (1993) Molecular piracy of mammalian interleukin-8 receptor type B by herpesvirus saimiri. J Biol Chem 268:20691-20694.
3. Arvanitakis L, Geras-Raaka E, Varma A, Gershengorn MC, and Cesarman E (1997) Human herpesvirus kshv encodes a constitutively active G-protein-coupled receptor linked to cell proliferation. Nature (Lond) 385:347-350.
4. Ballesteros JA, Jensen AD, Liapakis G, Rasmussen SG, Shi L, Gether U, and Javitch JA (2001) Activation of the β2-adrenergic receptor involves disruption of an ionic lock between the cytoplasmic ends of transmembrane segments 3 and 6. J Biol Chem 276:29171-29177.
5. Burger M, Burger JA, Hoch RC, Oades Z, Takamori H, and Schraufstatter IU (1999) Point mutation causing constitutive signalling of CXCR2 leads to transforming activity similar to Kaposi's sarcoma herpesvirus-G-protein-coupled receptor. J Immunol 163:2017-2022.
6. Capra V, Veltri A, Foglia C, Crimaldi L, Habib A, Parenti M, and Rovati GE (2004) Mutational analysis of the highly conserved ERY motif of the thromboxane A2 receptor: alternative role in G protein-coupled receptor signaling. Mol Pharmacol 66:880-889.
7. Casarosa P, Menge WM, Minisini R, Otto C, van Heteren J, Jongejan A, Timmerman H, Moepps B, Kirchhoff F, Mertens T, et al. (2003) Identification of the first nonpeptidergic inverse agonist for a constitutively active viral-encoded G protein-coupled receptor. J Biol Chem 278:5172-5178.
8. Chen A, Gao ZG, Barak D, Liang BT, and Jacobson KA (2001) Constitutive activation of A(3) adenosine receptors by site-directed mutagenesis. Biochem Biophys Res Commun 284:596-601.
9. α that alter the fidelity of receptor activation. Mol Pharmacol 50:885-890.
10. Davis-Poynter NJ, Lynch DM, Vally H, Shellam GR, Rawlinson WD, Barrell BG, and Farrell HE (1997) Identification and characterization of a G protein-coupled receptor homolog encoded by murine cytomegalovirus. J Virol 71:1521-1529.
11. Elling CE, Nielsen SM, and Schwartz TW (1995) Conversion of antagonist-binding site to metal-ion site in the tachykinin NK-1 receptor. Nature (Lond) 374:74-77.
12. Elling CE, Thirstrup K, Holst B, and Schwartz TW (1999) Conversion of agonist site to metal-ion chelator site in the beta(2)-adrenergic receptor. Proc Natl Acad Sci USA 96:12322-12327.
13. Fanelli F, Barbier P, Zanchetta D, de Benedetti PG, and Chini B (1999) Activation mechanism of human oxytocin receptor: a combined study of experimental and computer-simulated mutagenesis. Mol Pharmacol 56:214-225.
14. Farrens DL, Altenbach C, Yang K, Hubbell WL, and Khorana HG (1996) Requirement of rigid-body motion of transmembrane helices for light activation of rhodopsin. Science (Wash DC) 274:768-770.
15. Fritze O, Filipek S, Kuksa V, Palczewski K, Hofmann KP, and Ernst OP (2003) Role of the conserved NPxxY(x)5,6F motif in the rhodopsin ground state and during activation. Proc Natl Acad Sci USA 100:2290-2295.
16. Gruijthuijsen YK, Beuken EV, Smit MJ, Leurs R, Bruggeman CA, and Vink C (2004) Mutational analysis of the R33-encoded G protein-coupled receptor of rat cytomegalovirus: identification of amino acid residues critical for cellular localization and ligand-independent signalling. J Gen Virol 85:897-909.
17. Hill SJ, Baker JG, and Rees S (2001) Reporter-gene systems for the study of G-protein-coupled receptors. Curr Opin Pharmacol 1:526-532.
18. Holst PJ, Rosenkilde MM, Manfra D, Chen SC, Wiekowski MT, Holst B, Cifire F, Lipp M, Schwartz TW, and Lira SA (2001) Tumorigenesis induced by the HHV8-encoded chemokine receptor requires ligand modulation of high constitutive activity. J Clin Investig 108:1789-1796.
19. Kledal TN, Rosenkilde MM, Coulin F, Simmons G, Johnsen AH, Alouani S, Power CA, Lüttichau HR, Gerstoft J, Clapham PR, et al. (1997) A broad-spectrum chemokine antagonist encoded by Kaposi's sarcoma-associated herpesvirus. Science (Wash DC) 277:1656-1659.
20. Kostenis E (2001) Is Gα16 the optimal tool for fishing ligands of orphan G-protein-coupled receptors? Trends Pharmacol Sci 22:560-564.
21. Lee BJ, Koszinowski UH, Sarawar SR, and Adler H (2003) A gammaherpesvirus G protein-coupled receptor homologue is required for increased viral replication in response to chemokines and efficient reactivation from latency. J Immunol 170:243-251.
22. Lu ZL, Curtis CA, Jones PG, Pavia J, and Hulme EC (1997) The role of the aspartate-arginine-tyrosine triad in the M1 muscarinic receptor: mutations of aspartate 122 and tyrosine 124 decrease receptor expression but do not abolish signaling. Mol Pharmacol 51:234-241.
23. McLean KA, Holst PJ, Martini L, Schwartz TW, and Rosenkilde MM (2004) Similar activation of signal transduction pathways by the herpesvirus-encoded chemokine receptors US28 and ORF74. Virology 325:241-251.
24. Mirzadegan T, Benko G, Filipek S, and Palczewski K (2003) Sequence analyses of G-protein-coupled receptors: similarities to rhodopsin. Biochemistry 42:2759-2767.
25. Murphy PM, Baggiolini M, Charo IF, Hebert CA, Horuk R, Matsushima K, Miller LH, Oppenheim JJ, and Power CA (2000) International Union of Pharmacology. XXII nomenclature for chemokine receptors. Pharmacol Rev 52:145-176.
26. Okada T and Palczewski K (2001) Crystal structure of rhodopsin: implications for vision and beyond. Curr Opin Struct Biol 11:420-426.
27. Okinaga S, Slattery D, Humbles A, Zsengeller Z, Morteau O, Kinrade MB, Brodbeck RM, Krause JE, Choe HR, Gerard NP, et al. (2003) C5L2, a nonsignaling c5a binding protein. Biochemistry 42:9406-9415.
28. Rosenkilde MM, Kledal TN, Bräuner-Osborne H, and Schwartz TW (1999) Agonist and inverse agonist for the herpesvirus 8-encoded constitutively active seven-transmembrane oncogene product, ORF-74. J Biol Chem 274:956-961.
29. Rosenkilde MM, Lucibello M, Holst B, and Schwartz TW (1998) Natural agonist enhancing bis-his zinc-site in transmembrane segment v of the tachykinin NK3 receptor. FEBS Lett 439:35-40.
30. Rosenkilde MM, McLean KA, Holst PJ, and Schwartz TW (2004) The CXC chemokine receptor encoded by herpesvirus saimiri, ECRF3, shows ligand-regulated signaling through Gi, Gq and G12/13 proteins but constitutive signaling only through Gi and G12/13 proteins. J Biol Chem 279:32524-32533.
31. Rosenthal W, Antaramian A, Gilbert S, and Birnbaumer M (1993) Nephrogenic diabetes insipidus. A V2 vasopressin receptor unable to stimulate adenylyl cyclase. J Biol Chem 268:13030-13033.
32. Scheer A, Costa T, Fanelli F, de Benedetti PG, Mhaouty-Kodja S, Abuin L, Nenniger-Tosato M, and Cotecchia S (2000) Mutational analysis of the highly conserved arginine within the Glu/Asp-Arg-Tyr motif of the alpha(1b)-adrenergic receptor: effects on receptor isomerization and activation. Mol Pharmacol 57:219-231.
33. Scheer A, Fanelli F, Costa T, and Cotecchia S (1996) Constitutively active mutants of the alpha1b-adrenergic receptor: role of highly conserved polar amino acids in receptor activation. EMBO (Eur Mol Biol Organ) J 15:3566-3578.
34. Schwartz TW and Holst B (2003) Molecular structure and function of 7TM G-protein-coupled receptors, in Textbook of Receptor Pharmacology (Foreman JC and Johansen T eds) pp 81-110, CRC Press, Boca Raton, FL.
35. Schwarz M and Murphy PM (2001) Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor constitutively activates NF-κB and induces proinflammatory cytokine and chemokine production via a C-terminal signaling determinant. J Immunol 167:505-513.
36. Seibold A, Dagarag M, and Birnbaumer M (1998) Mutations of the DRY motif that preserve beta 2-adrenoceptor coupling. Recept Channels 5:375-385.
37. Shi L, Liapakis G, Xu R, Guarnieri F, Ballesteros JA, and Javitch JA (2002) β2 Adrenergic receptor activation. modulation of the proline kink in transmembrane 6 by a rotamer toggle switch. J Biol Chem 277:40989-40996.
38. Telford EA, Watson MS, Aird HC, Perry J, and Davison AJ (1995) The DNA sequence of equine herpesvirus 2. J Mol Biol 249:520-528.
39. Verzijl D, Fitzsimons CP, Van Dijk M, Stewart JP, Timmerman H, Smit MJ, and Leurs R (2004) Differential activation of murine herpesvirus 68- and Kaposi's sarcoma-associated herpesvirus-encoded ORF74 G protein-coupled receptors by human and murine chemokines. J Virol 78:3343-3351.
40. Wess J (1998) Molecular basis of receptor/G-protein-coupling selectivity. Pharmacol Ther 80:231-264.