An anchor site-type defect in human telomerase that disrupts telomere length maintenance and cellular immortalization

Molecular Biology of the Cell

Volumn 16, Issue 7, 2005, Pages 3152-3161

  Moriarty, Tara J ^a,b   Ward, Ryan J ^a,b   Taboski, Michael A S ^a,b,c   Autexier, Chantal ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PRIMER DNA; RNA; TELOMERASE; TELOMERASE REVERSE TRANSCRIPTASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL IMMORTALIZATION; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVITY; EUKARYOTIC CELL; HUMAN; HUMAN CELL; MUTATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN RNA BINDING; RNA INTERACTION DOMAIN 1; TELOMERE;

AMINO ACID MOTIFS; CATALYSIS; CATALYTIC DOMAIN; CELL LINE; DNA; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; IMMUNOBLOTTING; MUTATION; PHENOTYPE; PROMOTER REGIONS (GENETICS); PROTEIN STRUCTURE, TERTIARY; RETROVIRIDAE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TELOMERASE; TELOMERE; TIME FACTORS;

EUKARYOTA;

EID: 21844457135     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E05-02-0148     Document Type: Article

References (53)

1. Armbruster, B., Banik, S., Guo, C., Smith, A., and Counter, C. (2001). N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo. Mol. Cell. Biol. 21, 7775-7786.
2. Armbruster, B. N., Etheridge, K. T., Broccoli, D., and Counter, C. M. (2003). Putative telomere-recruiting domain in the catalytic subunit of human telomerase. Mol. Cell. Biol. 23, 3237-3246.
3. Armbruster, B. N., Linardic, C. M., Veldman, T., Bansal, N. P., Downie, D. L., and Counter, C. M. (2004). Rescue of an hTERT mutant defective in telomere elongation by fusion with hPot1. Mol. Cell. Biol. 24, 3552-3561.
4. Bachand, F., and Autexier, C. (1999). Functional reconstitution of human telomerase expressed in Saccharomyces cerevisiae. J. Biol. Chem. 274, 38027-38031.
5. Banik, S.S.R., Guo, C., Smith, A. C., Margolis, S. S., Richardson, D. A., Tirado, C. A., and Counter, C. M. (2002). C-terminal regions of the human telomerase catalytic subunit essential for in vivo enzyme activity. Mol. Cell. Biol. 22, 6234-6246.
6. Baran, N., Haviv, Y., Paul, B., and Manor, H. (2002). Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme. Nucleic Acids Res. 30, 5570-5578.
7. Baumann, P., and Cech, T. R. (2001). Pot1, the putative telomere end-binding protein in fission yeast and humans. Science 292, 1171-1175.
8. Beattie, T., Zhou, W., Robinson, M., and Harrington, L. (2000). Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Mol. Biol. Cell 11, 3329-3340.
9. Beattie, T., Zhou, W., Robinson, M., and Harrington, L. (2001). Functional multimerization of the human telomerase reverse transcriptase. Mol. Cell. Biol. 21, 6151-6160.
10. Bryan, T. M., Englezou, A., Gupta, J., Bacchetti, S., and Reddel, R. R. (1995). Telomere elongation in immortal human cells without detectable telomerase activity. EMBO J. 14, 4240-4248.
11. Collins, K. (1999). Ciliate telomerase biochemistry. Annu. Rev. Biochem. 68, 187-218.
12. Collins, K., and Greider, C. W. (1993). Tetrahymena telomerase catalyzes nucleolytic cleavage and non-processive elongation. Genes Dev. 7, 1364-1376.
13. Counter, C. M., Hahn, W. C., Wei, W., Dickinson Caddie, S., Beijersbergen, R. L., Lansdorp, P. M., Sedivy, J. M., and Weinberg, R. A. (1998). Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc. Natl. Acad. Sci. USA 95, 14723-14728.
14. Ford, L., Suh, J., Wright, W., and Shay, J. (2000). Heterogeneous nuclear ribonucleoproteins C1 and C2 associate with the RNA component of human telomerase. Mol. Cell. Biol. 20, 9084-9091.
15. Friedman, K. L., and Cech, T. R. (1999). Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants. Genes Dev. 13, 2863-2874.
16. Friedman, K. L., Heit, J. J., Long, D. M., and Cech, T. R. (2003). N-terminal domain of yeast telomerase reverse transcriptase: recruitment of Est3p to the telomerase complex. Mol. Biol. Cell 34, 1-13.
17. Hammond, P. W., Lively, T. N., and Cech, T. R. (1997). The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers. Mol. Cell. Biol. 17, 296-308.
18. Hardy, C. D., Schultz, C. S., and Collins, K. (2001). Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomerase. J. Biol. Chem. 276, 4863-4871.
19. Harley, C. B., Futcher, A. B., and Greider, C. W. (1990). Telomeres shorten during ageing of human fibroblasts. Nature 345, 458-460.
20. Harrington, L. (2003). Biochemical aspects of telomerase function. Cancer Lett. 194, 139-154.
21. Harrington, L., Zhou, W., McPhail, T., Oulton, R., Yeung, D.S.K., Mar, V., Bass, M. B., and Robinson, M. O. (1997). Human telomerase contains evolutionarily conserved catalytic and structural subunits. Genes Dev. 11, 3109-3115.
22. Harrington, L. A., and Greider, C. W. (1991). Telomerase primer specificity and chromosome healing. Nature 353, 451-454.
23. Hossain, S., Singh, S. M., and Lue, N. F. (2002). Functional analysis of the C-terminal extension of telomerase reverse transcriptase: a 'putative' thumb domain. J. Biol. Chem. 277, 36174-36180.
24. Huard, S., and Autexier, C. (2004). Human telomerase catalyzes nucleolytic primer cleavage. Nucleic Acids Res. 32, 2171-2180.
25. Huard, S., Moriarty, T. J., and Autexier, C. (2003). The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity. Nucleic Acids Res. 31, 4059-4070.
26. Kelleher, C., Kurth, I., and Lingner, J. (2005). Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro. Mol. Cell. Biol. 25, 808-818.
27. Kim, M., Xu, L., and Blackburn, E. H. (2003). Catalytically active human telomerase mutants with allele-specific biological properties. Exp. Cell Res. 288, 277-287.
28. Lai, C., Mitchell, J., and Collins, K. (2001). RNA binding domain of telomerase reverse transcriptase. Mol. Cell. Biol. 21, 990-1000.
29. Lee, M. S., and Blackburn, E. H. (1993). Sequence-specific DNA primer effects on telomerase polymerization activity. Mol. Cell. Biol. 13, 6586-6599.
30. Lee, S. R., Wong, M. Y., and Collins, K. (2003). Human telomerase reverse transcriptase motifs required for elongation of a telomeric substrate. J. Biol. Chem. 278, 52531-52536.
31. Lei, M., Zaug, A. J., Podell, E., and Cech, T. R. (2005). Switching human telomerase on and off with hPOT1 protein in vitro. J. Biol. Chem. 280, 20449-20456.
32. Licht, J. D., and Collins, K. (1999). Telomerase RNA function in recombinant Tetrahymena telomerase. Genes Dev. 13, 1116-1125.
33. Lue, N. F. (2004). Adding to the ends: what makes telomerase processive and how important is it? BioEssays 26, 955-962.
34. Lue, N. F., and Peng, Y. (1998). Negative regulation of yeast telomerase activity through an interaction with an upstream region of the DNA primer. Nucleic Acids Res. 26, 1487-1494.
35. Melek, M., Davis, B. T., and Shippen, D. E. (1994). Oligonucleotides complementary to the Oxytricha nova telomerase RNA delineate the template domain and uncover a novel mode of primer utilization. Mol. Cell. Biol. 14, 7827-7838.
36. Melek, M., Greene, E. C., and Shippen, D. E. (1996). Processing of nontelomeric 3′ ends by telomerase: default template alignment and endonucleolytic cleavage. Mol. Cell. Biol. 16, 3437-3445.
37. Miller, M. C., and Collins, K. (2002). Telomerase recognizes its template by using an adjacent RNA motif. Proc. Natl. Acad. Sci. USA 99, 6585-6590.
38. Moriarty, T. J., Dupuis, S., and Autexier, C. (2002a). Rapid upregulation of human telomerase activity in human leukemia HL-60 cells treated with clinical doses of the DNA-damaging drug etoposide. Leukemia 16, 1112-1120.
39. Moriarty, T. J., Huard, S., Dupuis, S., and Autexier, C. (2002b). Functional multimerization of human telomerase requires an RNA interaction domain in the N terminus of the catalytic subunit. Mol. Cell. Biol. 22, 1253-1265.
40. Moriarty, T. J., Marie-Egyptienne, D. T., and Autexier, C. (2004). Functional organization of repeat addition processivity and DNA synthesis determinants in the human telomerase multimer. Mol. Cell. Biol. 24, 3720-3733.
41. Morin, G. B. (1989). The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell 59, 521-529.
42. Morin, G. B. (1991). Recognition of a chromosome truncation site associated with α-thalassaemia by human telomerase. Nature 353, 454-456.
43. Oulton, R., and Harrington, L. (2004). A human telomerase-associated nuclease. Mol. Biol. Cell 15, 3244-3256.
44. Peng, Y., Mian, I., and Lue, N. (2001). Analysis of telomerase processivity: mechanistic similarity to HIV-1 reverse transcriptase and role in telomere maintenance. Mol. Cell 7, 1201-1211.
45. Rivera, M. A., and Blackburn, E. H. (2004). Processive utilization of the human telomerase template: lack of a requirement for template switching. J. Biol. Chem. 279, 53770-53781.
46. Singh, S. M., and Lue, N. F. (2003). Ever shorter telomere 1 (EST1)-dependent reverse transcription by Candida telomerase in vitro: evidence in support of an activating function. Proc. Natl. Acad. Sci. USA 100, 5718-5723.
47. Snow, B. E., Erdmann, N., Cruickshank, J., Goldman, H., Gill, R. M., Robinson, M. O., and Harrington, L. (2003). Functional conservation of the telomerase protein Est1p in humans. Curr. Biol. 13, 698-704.
48. Wallweber, G., Gryaznov, S., Pongracz, K., and Pruzan, R. (2003). Interaction of human telomerase with its primer substrate. Biochemistry 42, 589-600.
49. Wang, H., and Blackburn, E. H. (1997). De novo telomere addition by Tetrahymena telomerase in vitro. EMBO J. 16, 866-879.
50. Wang, H., Gilley, D., and Blackburn, E. H. (1998). A novel specificity for the primer-template pairing requirement in Tetrahymena telomerase. 17, 1152-1160.
51. Xia, J., Peng, Y., Mian, I., and Lue, N. (2000). Identification of functionally important domains in the N-terminal region of telomerase reverse transcriptase. Mol. Cell. Biol. 20, 5196-5207.
52. Yu, G.-L., and Blackburn, E. H. (1991). Developmentally programmed healing of chromosomes by telomerase in Tetrahymena. Cell 67, 823-832.
53. Zahler, A. M., Williamson, J. R., Cech, T. R., and Prescott, D. M. (1991). Inhibition of telomerase by G-quartet DNA structures. Nature 350, 718-720.