Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p

Molecular Genetics and Genomics

Volumn 273, Issue 5, 2005, Pages 361-370

  Brunson, Lee Ellen ^a   Dixon, Cheryl ^a   LeFebvre, Aaron ^a   Sun, Lisa ^a   Mathias, Neal ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Met30p; Met4p; Methionine biosynthesis; SCF ubiquitin ligase; WD 40 repeat motif

Indexed keywords

AMINO ACID; F BOX PROTEIN; METHIONINE; PROTEASOME; SULFUR; UBIQUITIN PROTEIN LIGASE;

ARTICLE; AUXOTROPHY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME SUBSTRATE; F BOX MOTIF; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FAMILY; PROTEIN INDUCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; TEMPERATURE SENSITIVE MUTANT; TEMPERATURE SENSITIVITY; UBIQUITINATION;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTORS; BLOTTING, WESTERN; DNA; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; GLUTATHIONE TRANSFERASE; METHIONINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; POINT MUTATION; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; REPRESSOR PROTEINS; S-PHASE KINASE-ASSOCIATED PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; UBIQUITIN; UBIQUITIN-PROTEIN LIGASE COMPLEXES;

EID: 21544451162     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-005-1137-6     Document Type: Article

References (39)

1. Brunson LE, Dixon C, Kozubowski L, Mathias N (2004) The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex. J Biol Chem 279:6674-6682
2. Ciechanover A (1998) The ubiquitin-proteasome pathway: on protein death and cell life. EMBO J 17:7151-7160
3. Deshaies RJ (1999) SCF and Cullin/Ring H2-based ubiquitin ligases. Annu Rev Cell Dev Biol 15:435-467
4. Dixon C, Brunson LE, Roy MM, Smothers D, Sehorn MG, Mathias N (2003) Overproduction of polypeptides corresponding to the amino terminus of the F-box proteins Cdc4p and Met30p inhibits ubiquitin ligase activities of their SCF complexes. Eukaryot Cell 2:123-133
5. Elbe R (1992) A simple and efficient procedure for transformation of yeasts. Biotechniques 13:18-20
6. Feldman RM, Correll CC, Kaplan KB, Deshaies RJ (1997) A complex of Cdc4p, Skp1p, and Cdc53p/Cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91:221-230
7. Flick K, Ouni I, Wohlschlegel JA, Capati C, McDonald WH, Yates JR, Kaiser P (2004) Proteolysis-independent regulation of the transcription factor Met4 by a single Lys 48-linked ubiquitin chain. Nat Cell Biol 6:634-641
8. Gaudet R, Bohm A, Sigler PB (1996) Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin. Cell 87:577-588
9. Glickman MH, Ciechanover A (2002) The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev 82:373-428
10. Kaiser P, Sia RA, Bardes EG, Lew DJ, Reed SI (1998) Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swel. Genes Dev 12:2587-2597
11. Kaiser P, Flick K, Wittenberg C, Reed SI (2000) Regulation of transcription by ubiquitination without proteolysis: Cdc34/ SCF(Met30)-mediated inactivation of the transcription factor Met4. Cell 102:303-314
12. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG Jr, Elledge SJ, Conaway RC, Harper JW, Conaway JW (1999) Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Science 284:657-661
13. Kitagawa M, Hatakeyama S, Shirane M, Matsumoto M, Ishida N, Hattori K, Nakamichi I, Kikuchi A, Nakayama K, Nakayama K (1999) An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin. EMBO J 18:2401-2410
14. Koepp DM, Harper JW, Elledge SJ (1999) How the cyclin became a cyclin: regulated proteolysis in the cell cycle. Cell 97:431-434
15. Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW, Elledge SJ (2001) Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294:173-177
16. Komachi K, Johnson AD (1997) Residues in the WD repeats of Tup1 required for interaction with alpha2. Mol Cell Biol 17:6023-6028
17. Kuras L, Rouillon A, Lee T, Barbey R, Tyers M, Thomas D (2002) Dual regulation of the met4 transcription factor by ubiquitin-dependent degradation and inhibition of promoter recruitment. Mol Cell 10:69-80
18. Liu Y, Mathias N, Steussy CN, Goebl MG (1995) Intragenic suppression among CDC34 (UBC3) mutations defines a class of ubiquitin-conjugating catalytic domains. Mol Cell Biol 15:5635-5644
19. Mathias N, Johnson SL, Winey M, Adams AEM, Goetsch L, Pringle JR, Byers B, Goebl MG (1996) Cdc53 acts in concert with Cdc4 and Cdc34 to control the G1 and S phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:6634-6643
20. Mathias N, Johnson S, Byers B, Goebl M (1999) The abundance of cell cycle regulatory protein Cdc4p is controlled by interactions between its F box and Skp1p. Mol Cell Biol 19:1759-1767
21. McMillan JN, Sia RA, Bardes ES, Lew DJ (1999) Phosphorylation-independent inhibition of Cdc28p by the tyrosine kinase Swe1p in the morphogenesis checkpoint. Mol Cell Biol 19:5981-5990
22. Mitchell DA, Marshall TK, Deschenes RJ (1993) Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast 9:715-723
23. Orlicky S, Tang X, Willems A, Tyers M, Sicheri F (2003) Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase. Cell 112:243-256
24. Passmore LA, Barford D (2004) Getting into position: the catalytic mechanisms of protein ubiquitylation. Biochem J 379:513-525
25. Patton EE, Willems AR, Sa D, Kuras L, Thomas D, Craig KL, Tyers M (1998) Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genes Dev 12:692-705
26. Pickart CM (2001) Mechanisms underlying ubiquitination. Annu Rev Biochem 70:503-533
27. Rose MD, Winston F, Heiter P (1990) Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY
28. Rouillon A, Barbey R, Patton EE, Tyers M, Thomas D (2000) Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex. EMBO J 19:282-294
29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
30. Schwob E, Bohm T, Mendenhall MD, Nasmyth K (1994) The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79:233-244
31. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Shevchenko A, Deshaies RJ (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13:1614-1626
32. Skowyra D, Craig KL, Tyers M, Elledge SJ, Harper JW (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91:209-219
33. Skowyra D, Koepp DM, Kamura T, Conrad MN, Conaway RC, Conaway JW, Elledge SJ, Harper JW (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284:662-665
34. Smothers DB, Kozubowski L, Dixon C, Goebl MG, Mathias N (2000) The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability. Mol Cell Biol 20:7845-7852
35. Thomas D, Surdin-Kerjan Y (1997) Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61:503-532
36. Verma R, Feldman RM, Deshaies RJ (1997) SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol Biol Cell 8:1427-1437
37. Wu G, Lyapina S, Das I, Li J, Gurney M, Pauley A, Chui I, Deshaies RJ, Kitajewski J (2001) SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation. Mol Cell Biol 21:7403-7415
38. Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW, Pavletich NP (2003) Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase. Mol Cell 11:1445-1456
39. Yaron A, Hatzubai A, Davis M, Lavon I, Amit S, Manning AM, Andersen JS, Mann M, Mercurio F, Ben-Neriah Y (1998) Identification of the receptor component of the IkappaBalpha-ubiquitin ligase. Nature 396:590-594