Metafectene is superior to lipofectamine in the transfection of G sα prostate cancer cells

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 28, Issue 2, 2004, Pages 97-103

  Iczkowski, Kenneth A ^a   Omara Opyene, A Levi ^a   Klösel, Roland ^a  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Lipofectamine; Lipofection; Metafectene; Prostate cancer; Transfection

Indexed keywords

AMINES; CELLS; FLUORESCENCE; GENES; RNA; STATISTICAL METHODS;

CANCER CELLS; LIPOFECTAMINE; LIPOFECTION; METAFECTENE; PROSTRATE CANCER;

BIOTECHNOLOGY;

CD44V ANTIGEN; DNA; GREEN FLUORESCENT PROTEIN; LIPOFECTAMINE; METAFECTENE; REAGENT; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; DOUBLE STRANDED RNA; DRUG CARRIER; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; LIPID;

ARTICLE; CANCER CELL CULTURE; CELL STRAIN; CONTROLLED STUDY; DNA VECTOR; EXPRESSION VECTOR; GENE CONSTRUCT; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONVIRAL GENE DELIVERY SYSTEM; NONVIRAL GENE THERAPY; NUCLEOTIDE SEQUENCE; PLASMID; PROSTATE CARCINOMA; REPORTER GENE; RNA INTERFERENCE; RNA STRUCTURE; BIOLOGICAL MODEL; BIOTECHNOLOGY; CHEMISTRY; GENE THERAPY; GENE VECTOR; MALE; METABOLISM; METHODOLOGY; PATHOLOGY; PROSTATE TUMOR; TIME; TUMOR CELL LINE; WESTERN BLOTTING;

BIOTECHNOLOGY; BLOTTING, WESTERN; CELL LINE, TUMOR; DNA; DRUG CARRIERS; GENE THERAPY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEIN ALPHA SUBUNITS; HUMANS; LIPIDS; MALE; MODELS, GENETIC; PLASMIDS; PROSTATIC NEOPLASMS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; TIME FACTORS; TRANSFECTION;

EID: 21344457489     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Sugimoto, C., Segev, L., and Franke, T. F. (2003) Metafectene method. Available at http://www.biontex.com. Accessed December 1, 2003.
2. Kogel, D., Schomburg, R., Schurmann, T., et al. (2003) The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis. J. Neurochem. 87, 248-256.
3. Park, J., Ries, J., Gelse, K., et al. (2003) Bone regeneration in critical size defects by cell-mediated BMP-2 gene transfer: a comparison of adenoviral vectors and liposomes. Gene Ther. 10, 1089-1098.
4. Taler, M., Shpungin, S., Salem, Y., Malovani, H., Pasder, O., and Nir, U. (2003) Fer is a downstream effector of insulin and mediates the activation of Stat3 in myogenic cells. Mol. Endocrinol. 17, 1580-1582.
5. Claus, P., Doring, F., Gringel, S., et al. (2003) Differential intranuclear localization of fibroblast growth factor-2 and specific interaction with the survival of motoneuron protein. J. Biol. Chem. 278, 479-485.
6. Miyagishi, M. and Taira, K. (2002) U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20, 497-500.
7. Guerra-Crespo, M., Charli, J. L., Rosales-Garcia, V. H., Pedraza-Alva, G., and Perez-Martinez, L. (2003) Polyethlyenimine improves the transfection efficiency of primary cultures of post-mitotic rat fetal hypothalamic neurons. J. Neurosci. Methods 127, 179-192.
8. sa] in proliferation of PC-3M prostate cancer cells. Int. J. Cancer 91, 46-54.
9. Kapadia, S. B., Brideau-Andersen, A., and Chisari, F. V. (2003) Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc. Natl. Acad. Sci. USA 100, 2014-2018.
10. Omara-Opyene, A. L. and Gedamu, L. (1997) Molecular cloning, characterisation and overexpression of two distinct cysteine protease cDNAs from Leishmania donovani chagasi. Mol. Biochem. Parasitol. 90, 247-267.
11. Iczkowski, K. A., Bai, S., and Pantazis, C. G. (2003) Prostate cancer overexpresses CD44 variants 7-9 at the messenger RNA and protein level. Anticancer Res. 23, 3129-3140.
12. Uchida, E., Mizuguchi, H., Ishii-Watabe, A., and Hayakawa, T. (2002) Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells. Biol. Pharm. Bull. 25, 891-897.
13. Escobedo, J. and Koh, T. J. (2003) Improved transfection technique for adherent cells using a commercial lipid reagent. Biotechniques 35, 936-940.
14. Merdan, T., Callahan, J., Petersen, H., et al. (2003) Pegylated polyethylenimine-fab' antibody fragment conjugates for targeted gene delivery to human ovarian carcinoma cells. Bioconjug. Chem. 14, 989-996.