Polyethylenimine improves the transfection efficiency of primary cultures of post-mitotic rat fetal hypothalamic neurons

Journal of Neuroscience Methods

Volumn 127, Issue 2, 2003, Pages 179-192

  Guerra Crespo, Magdalena ^a   Charli, Jean Louis ^a   Rosales García, Víctor Hugo ^b   Pedraza Alva, Gustavo ^a,c   Pérez Martínez, Leonor ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b DEPARTAMENTO DE FÍSICA   (Mexico)

^c UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

FACS; Hypothalamus; Lipofectamine 2000; Polyethylenimine; Primary culture; Transfection; TRH

Indexed keywords

GREEN FLUORESCENT PROTEIN; LIPOFECTAMINE; POLYETHYLENEIMINE; PROTIRELIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CYTOMEGALOVIRUS; FETUS; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONTROL; GENE SEQUENCE; GENETIC TRANSFECTION; HYPOTHALAMUS; MITOSIS; NERVE CELL; NONHUMAN; PH; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PURIFICATION; RAT; SEQUENCE ANALYSIS;

EID: 0041666314     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00125-0     Document Type: Article

References (46)

1. Balkan W., Tavianini M.A., Gkonos P.J., Roos B.A. Expression of rat thyrotropin-releasing hormone (TRH) gene in TRH-producing tissues of transgenic mice requires sequences located in exon 1. Endocrinology. 139:1998;252-259.
2. Benda P., De Vitry F., Picart R., Tixier-Vidal A. Dissociated cell cultures from fetal mouse hypothalamus: patterns of organization and ultrastructural features. Exp. Brain Res. 23:1975;29-47.
3. Boldyrev A., Song R., Lawrence D., Carpenter D.O. Carnosine protects against excitotoxic cell death independently of effects on reactive oxygen species. Neuroscience. 94:1999;571-577.
4. Boussif O., Lezoualc'h F., Zanta M.A., Mergny M.D., Scherman D., Demeneix B., Behr J.P. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. USA. 92:1995;7297-7301.
5. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
6. Chu G., Hayakawa H., Berg P. Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15:1987;1311-1326.
7. Cole R.L., Konradi C., Douglass J., Hyman S.E. Neuronal adaptation to amphetamine and dopamine: molecular mechanisms of prodynorphin gene regulation in rat striatum. Neuron. 14:1995;813-823.
8. Collas P., Alestrom P. Nuclear localization signal of SV40 T antigen directs import of plasmid DNA into sea urchin male pronuclei in vitro. Mol. Reprod. Dev. 45:1996;431-438.
9. Glover C.P., Bienemann A.S., Heywood D.J., Cosgrave A.S., Uney J.B. Adenoviral-mediated, high-level, cell-specific transgene expression: a SYN1-WPRE cassette mediates increased transgene expression with no loss of neuron specificity. Mol. Ther. 5:2002;509-516.
10. Goldfarb D.S., Gariepy J., Schoolnik G., Kornberg R.D. Synthetic peptides as nuclear localization signals. Nature. 322:1986;641-644.
11. Gorlich D., Mattaj I.W. Nucleocytoplasmic transport. Science. 271:1996;1513-1518.
12. + hypothalamic neurons in primary culture. Eur. J. Neurosci. 14:2001;483-494.
13. Horbinski C., Stachowiak M.K., Higgins D., Finnegan S.G. Polyethilenimine-mediated transfection of cultured postmitotic neurons from rat ganglia and adult human retina. BMC Neurosci. 2:2001;2-9.
14. Hurley M.J., Gerard D.J., Jauniaux E., Stern G.M., Uchida K., Bradford H.F. Cultured human foetal cerebral cortex, transfected with tyrosine hydroxylase cDNA, as a source of neural transplant material. J. Neural Transm. 108:2001;781-792.
15. Jaworski D.M., Kelly G.M., Hockfield S. Intracranial injury acutely induces the expression of the secreted isoform of the CNS-specific hyaluronan-binding protein BEHAB/brevican. Exp. Neurol. 157:1999;327-337.
16. Jiao S., Cheng L., Wolff J.A., Yang N.S. Particle bombardment-mediated gene transfer and expression in rat brain tissues. Biotechnology (NY). 11:1993;497-502.
17. Joseph-Bravo P., Pérez-Martínez L., Lezama L., Morales-Chapa C., Charli J.L. An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells. Brain Res. Protoc. 9:2002;93-104.
18. Kaech S., Kim J.B., Cariola M., Ralston E. Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. Mol. Brain Res. 35:1996;344-348.
19. Kichler A., Behr J.-P., Erbacher P. Polyethylenimines: a family of potent polymers for nucleic acid delivery. Huang L., Hung M.-C., Wagner E. Nonviral Vectors for Gene Therapy. 1999;192-206 Academic Press, San Diego, CA.
20. Kichler A., Leborgne C., Coeytaux E., Danos O. Polyethylenimine-mediated gene delivery: a mechanistic study. J. Gene Med. 3:2001;135-144.
21. Lambert R.C., Maulet Y., Dupont J.L., Mykita S., Craig P., Volsen, Feltz A. Polyethylenimine-mediated DNA transfection of peripheral and central neurons in primary culture: probing Ca2+ channel structure and function with antisense oligonucleotides. Mol. Cell Neurosci. 7:1996;239-246.
22. Legendre P., Tixier-Vidal A., Brigant J.L., Vincent J.D. Electrophysiology and ultrastructure of mouse hypothalamic neurons in culture: a correlative analysis during development. Brain Res. 471:1988;273-285.
23. Lezoualc'h F., Hassan A.H., Giraud P., Loeffler J.P., Lee S.L., Demeneix B.A. Assignment of the beta-thyroid hormone receptor to 3,5,3′-triiodothyronine-dependent inhibition of transcription from the thyrotropin-releasing hormone promoter in chick hypothalamic neurons. Mol. Endocrinol. 6:1992;1797-1804.
24. Li H., Chan S.T.H., Tang F. Transfection of rat brain by electroporation. J. Neurosci. Methods. 75:1997;29-32.
25. Liepelt U., Kindler-Rohrborn A., Lennartz K., Reinhardt-Maelicke S., Rajewsky M.F. Differentiation potential of a monoclonal antibody-defined neural progenitor cell population isolated from prenatal rat brain by fluorescence-activated cell sorting. Dev. Brain Res. 51:1990;267-278.
26. Litman P., Barg J., Rindzoonski L., Ginzburg I. Subcellular localization of tau mRNA in differentiating neuronal cell culture: implications for neuronal polarity. Neuron. 10:1993;627-638.
27. Liu Y.C., Storm D.R. Expression of a neuromodulin-beta-galactosidase fusion protein in primary cultured neurons and its accumulation in growth cones. Mol. Cell Biochem. 104:1991;29-34.
28. Meichsner M., Doll T., Reddy D., Weisshaar B., Matus A. The low molecular weight form of microtubule-associated protein 2 is transported into both axons and dendrites. Neuroscience. 54:1993;873-880.
29. Midoux P., Mendes C., Legrand A., Raimond J., Mayer R., Monsigny M., Roche A.C. Specific gene transfer mediated by lactosylated poly-L-lysine into hepatoma cells. Nucleic Acids Res. 21:1993;871-878.
30. Ohki E.C., Tilkins M.L., Ciccarone V.C., Price P.J. Improving the transfection efficiency of post-mitotic neurons. J. Neurosci. Methods. 112:2001;95-99.
31. Pérez-Martínez L., Carreón-Rodríguez A., González-Alzati M.E., Morales C., Charli J.L., Joseph-Bravo P. Dexamethasone rapidly regulates TRH mRNA levels in hypothalamic cell cultures: interaction with the cAMP pathway. Neuroendocrinology. 68:1998;345-354.
32. Pérez-Martínez L., Charli J.L., Joseph-Bravo P. Development of pro-TRH gene expression in primary cultures of fetal hypothalamic cells. Dev. Brain Res. 130:2001;73-81.
33. Pollard H., Remy J.S., Loussouarn G., Demolombe S., Behr J.P., Escande D. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J. Biol. Chem. 273:1998;7507-7511.
34. Rupprecht A.P., Coleman D.L. Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. J. Immunol. Methods. 144:1991;157-163.
35. Simonato M., Manservigi R., Marconi P., Glorioso J. Gene transfer into neurones for the molecular analysis of behaviour: focus on herpes simplex vectors. TINS. 23:2000;183-190.
36. Sola B., Staedel C., Remy J.S., Bahr A., Behr J.P. Lipospermine-mediated gene transfer technique into murine cultured cortical cells. J. Neurosci. Methods. 71:1997;183-186.
37. Tabuchi A., Sano K., Nakaoka R., Nakatani C., Tsuda M. Inducibility of BDNF gene promoter I detected by calcium-phosphate-mediated DNA transfection is confined to neuronal but not to glial cells. Biochem. Biophys. Res. Commun. 253:1998;818-823.
38. Tixier-Vidal A., Nemeskeri A., Faivre-Bauman A. Primary cultures of dispersed hypothalamic cells. Vincent J.D., Kordon C. Ultrastructural and Functional Features of Differentiation. Biologie Cellulaire des Processus Neurosécrétoires Hypothalamiques. 1978;777-801 Presses du C.N.R.S, Paris.
39. Uribe R.M., Perez-Martinez L., Covarrubias M.L., Gomez O., Covarrubias L., Charli J.L., Joseph-Bravo P. Phorbol ester or cAMP enhance thyrotropin-releasing hormone mRNA in primary cultures of hypothalamic cells. Neurosci. Lett. 201:1995;41-44.
40. Wang S., Roy N.S., Benraiss A., Goldman S.A. Promoter-based isolation and fluorescence-activated sorting of mitotic neuronal progenitor cells from the adult mammalian ependymal/subependymal zone. Dev. Neurosci. 22:2000;167-176.
41. Watanabe S.Y., Albsoul-Younes A.M., Kawano T., Itoh H., Kaziro Y., Nakajima S., Nakajima Y. Calcium phosphate-mediated transfection of primary cultured brain neurons using GFP expression as a marker: application for single neuron electrophysiology. Neurosci. Res. 33:1999;71-78.
42. Wiesenhofer B., Humpel C. Lipid-mediated gene transfer into primary neurons using fugene: comparison to C6 glioma cells and primary glia. Exp. Neurol. 164:2000;38-44.
43. Xia Z., Dudek H., Miranti C.K., Greenberg M.E. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J. Neurosci. 16:1996;5425-5436.
44. Yang K., Faustinella F., Xue J.J., Whitson J., Kampfl A., Mu X.S., Zhao X., Taglialatela G., Perez-Polo J.R., Clifton G., Hayes R.L. Optimizing liposome-mediated gene transfer in primary rat septo-hippocampal cell cultures. Neurosci. Lett. 182:1994;287-290.
45. Zanta M.A., Belguise-Valladier P., Behr J.P. Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus. Proc. Natl. Acad. Sci. USA. 96:1999;91-96.
46. Zingg J.M., Pedraza A.G., Jost J.P. Characterization of a genomic clone covering the structural mouse MyoD1 gene and its promoter region. Nucleic Acids Res. 19:1991;6433-6439.