High-throughput mass-spectrometry monitoring for multisubstrate enzymes: Determining the kinetic parameters and catalytic activities of glycosyltransferases

ChemBioChem

Volumn 6, Issue 2, 2005, Pages 346-357

  Yang, Min ^a   Brazier, Melissa ^b   Edwards, Robert ^b   Davis, Benjamin G ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b DURHAM UNIVERSITY   (United Kingdom)

Author keywords

Enzymes; Glyeosyltransferases; High throughput; Kinetics; Mass spectrornetry; Screening

Indexed keywords

ANTIBIOTIC AGENT; BENZOIC ACID DERIVATIVE; CARBOHYDRATE; COUMARIN DERIVATIVE; FLAVONOID; GLYCOSYLTRANSFERASE; GUANOSINE DIPHOSPHATE FUCOSE; GUANOSINE DIPHOSPHATE MANNOSE; LACTOSE SYNTHASE; PHYTOHORMONE; PROPIONIC ACID DERIVATIVE; PROTEIN UGT72B1; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE; URIDINE DIPHOSPHATE MANNOSE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; URIDINE DIPHOSPHATE SUGAR; URIDINE DIPHOSPHATE THIOGLUCOSE; VEGETABLE PROTEIN;

ARTICLE; CATALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HIGH THROUGHPUT SCREENING; MASS SPECTROMETRY; MONITORING; NONHUMAN; PRIORITY JOURNAL; TIME OF FLIGHT MASS SPECTROMETRY;

ANIMALS; CATTLE; GLYCOSYLTRANSFERASES; MOLECULAR PROBES; MOLECULAR STRUCTURE; NUCLEOSIDE DIPHOSPHATE SUGARS; PLANT PROTEINS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY;

ARABIDOPSIS THALIANA; BOVINAE;

EID: 21144450850     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200400100     Document Type: Article

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