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Vesicles of different size will contain different numbers of lipid molecules. The difference is actually quite significant, as it scales with the square of the ratio between the radii of the vesicles: at a given lipid concentration, there will be 16 times more 50 nm vesicles than 200 nm ones. Therefore it is strictly speaking incorrect to compare results obtained at the similar lipid concentrations. The relevant quantity that needs to be considered is the vesicle concentration, which also determines the adsorption rate of the vesicles on the surface. It was calculated from lipid concentration using the molecular weight of the lipid [786.15 g/mol for DOPC (Ref. 45)] and 204711-7 Vesicular layers supported on TiO2 J. Chem. Phys. 122, 204711 ~2005! area per lipid molecule [0.82 nm2 /molecule (Refs. 45,46)].
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