Formation of supported phospholipid bilayers from unilamellar vesicles investigated by atomic force microscopy

Langmuir

Volumn 16, Issue 4, 2000, Pages 1806-1815

  Reviakine, Ilya ^a   Brisson, Alain ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ATOMIC FORCE MICROSCOPY; CALCIUM; MULTILAYERS; PROTEINS; SURFACES; SUSPENSIONS (FLUIDS);

SONICATION; SUPPORTED PHOSPHOLIPIDS BILAYERS; UNILAMELLAR VESICLES; ZWITTERIONIC PHOSPHOLIPIDS;

LIPIDS;

EID: 0033894687     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la9903043     Document Type: Article

References (52)

1. Watts, T. H.; Brian, A. A.; Kappler, J. W.; Marrack, P.; McConnell, H. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 7564. Watts, T. H.; Gaub, H. E.; McConnell, H. M. Nature 1986, 320, 179.
2. Watts, T. H.; Brian, A. A.; Kappler, J. W.; Marrack, P.; McConnell, H. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 7564. Watts, T. H.; Gaub, H. E.; McConnell, H. M. Nature 1986, 320, 179.
3. Sackmann, E. Science 1996, 271, 43.
4. Kalb, E.; Frey, S.; Tamm, L. K. Biochim. Biophys. Acta 1992, 1103, 307.
5. Brink, G.; Schmitt, L.; Tampe, R.; Sackmann, E. Biochim. Biophys. Acta 1994, 1196, 227.
6. Cheng, Y.; Boden, N.; Bushby, R.; Clarkson, S.; Evans, S. D.; Knowles, P. F.; Marsh, A.; Miles, R. Langmuir 1998, 14, 839.
7. Bayerl, T. M.; Bloom, M. Biophys. J. 1990, 58, 357.
8. Müller, D. J.; Amrein, M.; Engel, A. J. Struct. Biol. 1997, 119, 172.
9. Nollert, P.; Kiefer, H.; Jähnig, F. Biophys. J. 1995, 69, 1447.
10. Keller, C. A.; Kasemo, B. Biophys. J. 1998, 75, 1397.
11. Keller, C. A.; Glasmaester, K.; Zhdanov, V. P.; Kasemo, B. Paper presented at the Centennial Meeting of the American Physical Society, March 1999, Atlanta, GA. Bull. Am. Phys. Soc. 1999, 44 (1) Part II, 1936. Manuscript submitted for publication.
12. Benz, J.; Düzgünes, N.; Nir, S. Biochemistry 1983, 22, 3320.
13. Reviakine, Mazères, Simon, and Brisson. Unpublished observations.
14. Seifert, U. Adv. Phys. 1997, 46, 13.
15. Lipowsky, R.; Seifert, U. Mol. Cryst. Liq. Cryst. 1991, 202, 17.
16. Shao, Z.; Mou, J.;Czajkowsky, D. M.; Yang, J.; Yuan, J.-Y. Adv. Phys. 1996, 45, 1.
17. Shao, Z.; Yang, J.; Somlyo, A. P. Annu. Rev. Cell Dev. Biol. 1995, 11, 241.
18. Reviakine, I.; Bergsma-Schutter, W.; Brisson, A. J. Struct. Biol. 1998, 121, 356.
19. Tiberg, F.; Landgren, M. Langmuir 1993, 9, 927.
20. Rädler, J.; Radmacher, M.; Gaub, H. E. Langmuir 1994, 10, 3111.
21. Mou, J.; Yang, J.; Shao, Z. J. Mol. Biol. 1995, 248, 507.
22. Sixma, T. K.; Pronk, S. E.; Kalk, K. H.; Wartna, E. S.; van Zanten, B. A. M.; Witholt, B.; Hol, W. G. J. Nature 1991, 351, 371.
23. Yeager, P. L. The Membranes of Cells, 2nd ed.; Academic Press: San Diego, CA, 1987.
24. E. coli lipids are composed of ∼85% phosphatidylethanolamine, 10% phopshatidylglycerol, and 5% cardiolipin. Both PG and CL are negatively charged.
25. 2+ on SPB formation discussed below refer to its presence on both sides of the bilayer.
26. The difference between the two methods - i and ii (see Experimental Section) - is due to the fact that the former does not necessarily represent the equilibrium situation, while the latter one does. The significance of these vesicles and of whether the system is in equilibrium or not is elaborated on in the Discussion.
27. 5 was more consistent (see figure legends). (The height of biological macromolecules as measured by AFM is discussed in ref 48). Second, vesicles are soft objects and are likely to be deformed by the tip during imaging.
28. Helfrich, W. Z. Naturforsch 1973, 28c, 693.
29. -7 mJ/m.
30. r. They will form single-bilayer disks. An intact vesicle adsorbed in close proximity to the "active edge" of such a single-bilayer disk will fuse with it (see the discussion of the late stages of SPB formation below). At high enough lipid concentrations, this process will lead to the formation of an SPB.
31. At the low lipid concentrations and small time scales involved in the current study, fusion of SUVs and - especially - EUVs in solution is considered to be unlikely.
32. It is intuitively clear that this process will also depend on the lipid concentration, since higher concentrations increase the probability of contact between vesicles. In fact, this is the only step in the formation of single-bilayer disks (adsorption of vesicles, their fusion, disk formation by vesicle rupture) which is concentration-dependent. However, the definition of "contact" is not trivial in this case, and it is therefore difficult to take the concentration dependence into account.
33. 47
34. Wilschut, J.; Düzgünes, N.; Papahadjopoulos, D. Biochemistry 1981, 20, 3126.
35. Day, E. P.; Kwok, A. Y. W.; Hark, S. K.; Ho, J. T.; Vail, W. J.; Bentz, J.; Nir, S. Proc. Natl. Acad. Sci. U.S.A. 1980, 77 (7), 4026.
36. Leckband, D. E.; Helm, C. A.; Israelachvili, J. Biochemistry 1993, 32, 1127.
37. Helm, C. A.; Israelachvili, J. N.; McGuiggan, P.M. Science 1989, 246, 919.
38. Lis, L. J.; Lis, T. W.; Parasegian, V. A.; Rand, R. P. Biochemistry 1981, 20, 1771.
39. Remaining (small) defects are thought to be due to the local properties of mica. See also ref 20.
40. Rinia, H. A.; Demel, R. A.; van der Eerden, J. P. J. M.; de Kruijff, B. Biophys. J. 1999, 77, 1683.
41. Reviakine, I.; Simon, A.; Brisson, A. Langmuir, in press.
42. Mou, J.; Yang, J.; Huang, C.; Shao, Z. Biochemistry 1994, 33 (3), 9981.
43. Fang, Y.; Yang, J. Biochim. Biophys. Acta 1997, 1324, 309.
44. Annexins: Molecular Structure to Cellular Function; Seaton, B. A., Ed.; Chapman and Hall: New York, 1996.
45. At the low lipid concentration used, fusion between surface-bound vesicles, discussed in detail above, is expected to be negligible.
46. Egawa, H.; Furusawa, K. Langmuir 1999, 15, 1660.
47. Niggermann, G.; Kummrow, M.; Helfrich, W. J. Phys. II 1995, 5, 413.
48. Templer, R. H.; Khoo, B. J.; Seddon, J. M. Langmuir 1998, 14, 7427.
49. Müller, D. J.; Engel, A. Biophys. J. 1997, 73, 1633.
50. Israelachvili, J. Intermolecular and Surface Forces, 2nd ed.; Academic Press: London, 1992.
51. Giesen, P. L. A. Production of Thrombin at Macroscopic Surfaces. Ph.D. Thesis, University of Limburg, 1992.
52. A few (one or two) small disks could sometimes be found on samples and are thought to be due to contamination or tip-induced rupture.