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In the present case, the kinetics of adsorption, i.e., the supply to the surface of lipid mass in the form of vesicles, is diffusion limited for most of the time. Diffusion-limited adsorption often prevents the true adsorption kinetics from being observed, unless the transport to the surface is reliably modeled and corrected for. However, in the present case the kinetics on the surface, e.g., vesicle to SPB transformation, which is of prime interest here, can still be followed thanks to the information contained in f and D.
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1/2, to account for the T-dependent diffusion rate. This correction has only a minor influence on the results discussed below. At t = 40 (n.u.) the clock time is 144 and 138 s at 278 and 303 K, respectively.
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63
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0345142398
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note
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Qualitatively similar results were obtained for 25 nm SUVs (not shown), except that the curve shapes were somewhat different. The width and depth of the minimum in f/maximum in D generally depend on size, vesicle composition, and vesicle manufacturing method.
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64
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0345142399
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Both f and D scale with the square root of density times viscosity when exposed to a Newtonian liquid
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Both f and D scale with the square root of density times viscosity when exposed to a Newtonian liquid.
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Note that the Sauerbrey relation is a very good approximation for the planar bilayer, since the bilayer is much thinner than the extinction depth of the probing evanescent acoustic wave in the liquid, and it is also sufficiently rigid to cause very low dissipation.
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2 at 295 K with the time to the corresponding magnitude compared to the saturated vesicle coverage at 273 K. This might underestimate the coverage if the deformation of the vesicles is substantially lower at 273 K.
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2. It might also be a factor to consider reducing the number of nonruptured vesicles on the surface after SPB formation.
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