Homogeneous assays for cellular proteases employing the platinum(II)-coproporphyrin label and time-resolved phosphorescence

Analytical Biochemistry

Volumn 342, Issue 1, 2005, Pages 111-119

  O'Riordan, Tomás C ^a   Hynes, James ^a   Yashunski, Dmitri ^b   Ponomarev, Gelii V ^b   Papkovsky, Dmitri B ^a,c  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b INSTITUTE OF BIOMEDICAL CHEMISTRY   (Russian Federation)

^c Luxcel Biosciences Ltd   (Ireland)

Author keywords

Caspase; Homogeneous assays; Labels; Metalloporphyrin; Phosphorescence; Protease assays; Time resolved fluorescence

Indexed keywords

CELL CULTURE; DRUG DOSAGE; FLUORESCENCE; LABELING; PHOSPHORESCENCE; PORPHYRINS;

CASPASES; HOMOGENEOUS ASSAYS; METALLOPORPHYRINS; PROTEASE ASSAY; TIME-RESOLVED FLUORESCENCE;

PLATINUM COMPOUNDS;

CASPASE 3; COPROPORPHYRIN; MALEIMIDE; PLATINUM COMPLEX; PROTEINASE;

ARTICLE; CELL LABELING; CELL LINE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME DEGRADATION; FLUORESCENCE ANALYSIS; FLUOROMETRY; HUMAN; HUMAN CELL; INTRACELLULAR SPACE; PHOSPHORESCENCE; PRIORITY JOURNAL; PROTEIN MOTIF;

EID: 20444371561     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2005.04.016     Document Type: Article

References (32)

1. H. Bazin, M. Preaudat, E. Trinquet, and G. Mathis Homogeneous time resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology Spectrochim. Acta A Mol. Biomol. Spectrosc. 57 2001 2197 2211
2. N.A. Thornberry, H.G. Bull, J.R. Calaycay, K.T. Chapman, A.D. Howard, M.J. Kostura, D.K. Miller, S.M. Molineaux, J.R. Weidner, and J. Aunins A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes Nature 356 1992 768 774
3. V. Gurtu, S.R. Kain, and G. Zhang Fluorometric and colorimetric detection of caspase activity associated with apoptosis Anal. Biochem. 251 1997 98 102
4. M.W. Pennington, and N.A. Thornberry Synthesis of a fluorogenic interleukin-1 beta converting enzyme substrate based on resonance energy transfer Peptide Res. 7 1994 72 76
5. H. Hug, M. Los, W. Hirt, and K.M. Debatin Rhodamine110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells Biochemistry 38 1999 13906 13911
6. J. Liu, M. Bhalgat, C. Zhang, Z. Diwu, B. Hoyland, and D.H. Klaubert Fluorescent molecular probes: V.A sensitive caspase-3 substrate for fluorometric assays Bioorg. Med. Chem. Lett. 9 1999 3231 3236
7. S. Mittoo, L.E. Sundstrom, and M. Bradley Synthesis and evaluation of fluorescent probes for the detection of calpain activity Anal. Biochem. 319 2003 234 238
8. J. Jones, R. Heim, E. Hare, J. Stack, and B.A. Pollok Development and application of a GFP-FRET intracellular caspase assay for drug screening J. Biomol. Screen. 5 2000 307 318
9. I.A. Hemmila Applications of fluorescence in immunoassays 1991 John Wiley New York
10. I. Hemmila, and V.-M. Mukkala Time-resolution in fluorometry technologies, labels, and applications in bioanalytical assays Crit. Rev. Clin. Lab. Sci. 38 2001 441 519
11. J. Karvinen, V. Laitala, M.L. Makinen, O. Mulari, J. Tamminen, J. Hermonen, P. Hurskainen, and I. Hemmila Fluorescence quenching-based assays for hydrolyzing enzymes: Application of time-resolved fluorometry in assays for caspase, helicase, and phosphatase Anal. Chem. 76 2004 1429 1436
12. J. Karvinen, A. Elomaa, M.L. Makinen, H. Hakala, V.M. Mukkala, J. Peuralahti, P. Hurskainen, J. Hovinen, and I. Hemmila Caspase multiplexing: Simultaneous homogeneous time-resolved quenching assay (TruPoint) for caspases 1, 3, and 6 Anal. Biochem. 325 2004 317 325
13. S.M. Gopalakrishnan, J. Karvinen, J.L. Kofron, D.J. Burns, and U. Warrior Application of micro arrayed compound screening (microARCS) to identify inhibitors of caspase-3 J. Biomol. Screen. 7 2002 317 323
14. J. Peuralahti, H. Hakala, V.M. Mukkala, K. Loman, P. Hurskainen, O. Mulari, and J. Hovinen Introduction of lanthanide(III) chelates to oligopeptides on solid phase Bioconj. Chem. 13 2002 870 875
15. M. Preaudat, J. Ouled-Diaf, B. Alpha-Bazin, G. Mathis, T. Mitsugi, Y. Aono, K. Takahashi, and H. Takemoto A homogeneous caspase-3 activity assay using HTRF technology J. Biomol. Screen. 7 2002 267 274
16. J. Karvinen, P. Hurskainen, S. Gopalakrishnan, D. Burns, U. Warrior, and I. Hemmila Homogeneous time-resolved fluorescence quenching assay (LANCE) for caspase-3 J. Biomol. Screen. 7 2002 223 231
17. T.C. O'Riordan, A.E. Soini, and D.B. Papkovsky Monofunctional derivatives of coproporphyrins for phosphorescent labeling of proteins and binding assays Anal. Biochem. 290 2001 366 375
18. D. Eastwood, and M. Gouterman Porphyrins: XVIII. Luminescence of (Co), (Ni), Pd, Pt complexes J. Mol. Spectr. 35 1970 359 375
19. A.E. Soini, D.V. Yashunsky, N.J. Meltola, and G.V. Ponomarev Preparation of monofunctional and phosphorescent palladium(II) and platinum(II) coproporphyrin labeling reagents J. Porphyrins Phthalocyanines 5 2001 735 741
20. T.C. O'Riordan, A.E. Soini, J.T. Soini, and D.B. Papkovsky Performance evaluation of the phosphorescent porphyrin label: Solid-phase immunoassay of α-fetoprotein Anal. Chem. 74 2002 5845 5850
21. P.J. O'Sullivan, M. Burke, A.E. Soini, and D.B. Papkovsky Synthesis and evaluation of phosphorescent oligonucleotide probes for hybridisation assays Nucleic Acids Res. 30 2002 e114
22. M. Burke, P.J. O'Sullivan, A.E. Soini, H. Berney, and D.B. Papkovsky Evaluation of the phosphorescent palladium(II) coproporphyrin labels in separation-free hybridization assays Anal. Biochem. 320 2003 273 280
23. A.E. Soini, L. Seveus, N.J. Meltola, D.B. Papkovsky, and E. Soini Phosphorescent metalloporphyrins as labels in time-resolved luminescence microscopy: Effect of mounting on emission intensity Microsc. Res. Tech. 58 2002 125 131
24. N.A. Thornberry, and Y. Lazebnik Caspases: Enemies within Science 281 1998 1312 1316
25. D.W. Nicholson Caspase structure, proteolytic substrates, and function during apoptotic cell death Cell Death Differ. 6 1999 1028 1042
26. G.M. Cohen Caspases: The executioners of apoptosis Biochem. J. 326 1997 1 16
27. R.V. Talanian, C. Quinlan, S. Trautz, M.C. Hackett, J.A. Mankovich, D. Banach, T. Ghayur, K.D. Brady, and W.W. Wong Substrate specificities of caspase family proteases J. Biol. Chem. 272 1997 9677 9682
28. J.R. Lakowicz Principles of fluorescence spectroscopy 2nd ed. 1999 Kluwer Academic New York
29. S.B. Brown, M. Shillcock, and P. Jones Equilibrium and kinetic studies of the aggregation of porphyrins in aqueous solution Biochem. J. 153 1976 279 285
30. J.M. Vanderkooi, G. Maniara, T.J. Green, and D.F. Wilson An optical method for measurement of dioxygen concentration based upon quenching of phosphorescence J. Biol. Chem. 262 1987 5476 5482
31. J.H. Zhang, T.D. Chung, and K.R. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screen. 4 1999 67 73
32. A.E. Soini, A. Kuusisto, N.J. Meltola, E. Soini, and L. Seveus A new technique for multiparameter imaging microscopy: Use of long decay time photoluminescent labels enables multiple color immunocytochemistry with low channel-to-channel crosstalk Microsc. Res. Tech. 62 2003 396 407