Improvement of bioremediation by Pseudomonas and Burkholderia by mutants of the Vitreoscilla hemoglobin gene (vgb) integrated into their chromosomes

Journal of Industrial Microbiology and Biotechnology

Volumn 32, Issue 4, 2005, Pages 148-154

  Kim, Yongsoon ^a,b   Webster, Dale A ^a   Stark, Benjamin C ^a  

^a Illinois Institute of Technology   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

2,4 Dinitrotoluene; Bacterial hemoglobin; Benzoic acid; Oxygen affinity; Vitreoscilla hemoglobin

Indexed keywords

2,4 DINITROTOLUENE; BACTERIAL PROTEIN; BENZOIC ACID; HEMOGLOBIN; MUTANT PROTEIN;

AERATION; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM MUTANT; BIODEGRADATION; BIOREMEDIATION; BURKHOLDERIA; CHROMOSOME; DNA INTEGRATION; FUNGUS GROWTH; GENETIC ENGINEERING; OXYGEN AFFINITY; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; STRAIN DIFFERENCE; VITREOSCILLA; WILD TYPE;

BACTERIAL PROTEINS; BENZOIC ACID; BIODEGRADATION, ENVIRONMENTAL; BURKHOLDERIA; CHROMOSOMES, BACTERIAL; CONJUGATION, GENETIC; DINITROBENZENES; GENETIC ENGINEERING; HEMOGLOBINS; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXYGEN; PSEUDOMONAS; TRANSFORMATION, GENETIC; VITREOSCILLA;

BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA SP.; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; VITREOSCILLA;

EID: 20044366215     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10295-005-0215-4     Document Type: Article

References (29)

1. Andersson CIJ, Holmberg N, Farres J, Bailey JE, Bulow L, Kallio PT (2000) Error-prone PCR of Vitreoscilla hemoglobin (VHb) to support the growth of microaerobic Escherichia coli. Biotechnol Bioeng 70:446-455
2. 31 P NMR and saturation transfer study. Biotechnol Prog 10:360-364
3. Chung JW, Webster DA, Pagilla KR, Stark BC (2001) Chromosomal integration of the Vitreoscilla hemoglobin gene in Burkholderia and Pseudomonas for the purpose of producing stable engineered strains with enhanced bioremediating ability. J Ind Microbiol Biotechnol 27:27-33
4. De Lorenzo V, Herrero M, Jakubzik U, Timmis KN (1990) Mini-Tn 5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J Bacteriol 172:6568-6572
5. Dikshit KL, Webster DA (1988) Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli. Gene 70:377-386
6. Dikshit KL, Orii Y, Navani N, Patel S, Huang H-Y, Stark BC, Webster DA (1998) Site-directed mutagenesis of bacterial hemoglobin: the role of glutamine (E7) in oxygen-binding in the distal heme pocket. Arch Biochem Biophys 349:161-166
7. Fish PA, Webster DA, Stark BC (2000) Vitreoscilla hemoglobin enhances the first step in 2,4-dinitrotoluene degradation in vitro and at low aeration in vivo. J Mol Catal B 9:75-82
8. Hardison RC (1996) A brief history of hemoglobins: plant, animal, protist, and bacteria. Proc Natl Acad Sci USA 93:5675-5679
9. Herrero M, de Lorenzo V, Timmis KN (1990) Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J Bacteriol 172:6557-6567
10. Kaur R, Pathania R, Sharma V, Mande SC, Dikshit KL (2002) Chimeric Vitreoscilla hemoglobin (VHb) carrying a flavoreductase domain relieves nitrosative stress in Escherichia coli: new insight into the functional role of VHb. Appl Environ Microbiol 68:152-160
11. Kita K, Konishi K, Anraku Y (1984) Terminal oxidases of Escherichia coli aerobic respiratory chain II. Purification and properties of cytochrome b558-d complex from cells grown with limited oxygen and evidence of branched electron-carrying systems. J Biol Chem 259:3375-3381
12. Lee SY, Stark BC, Webster DA (2004) Structure-function studies of the Vitreoscilla hemoglobin D-region. Biochem Biophys Res Commun 316:1101-1106
13. Lin ECC (1999) Regulation of fermentation and respiration. In: Lengeler JW, Drews G, Schlegel HG (eds) Biology of the prokaryotes. Blackwell, New York, pp 524-537
14. Lin JM, Stark BC, Webster DA (2003) Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (DNT) dioxygenase activity of Burkholderia and on DNT degradation in two phase bioreactors. J Ind Microbiol Biotechnol 30:362-368
15. Miller JP (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
16. Miller VL, Mekalanos JJ (1988) A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires tox R. J Bacteriol 170:2575-2583
17. Nasr MA, Hwang KW, Akbas M, Webster DA, Stark BC (2001) Effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by Burkholderia engineered with the Vitreoscilla hemoglobin gene. Biotechnol Prog 17:359-361
18. Park KW, Kim KJ, Howard AJ, Stark BC, Webster DA (2002) Vitreoscilla hemoglobin binds to subunit I of cytochrome bo ubiquinol oxidases. J Biol Chem 277:33334-33337
19. Patel SM, Stark BC, Hwang KW, Dikshit KL, Webster DA (2000) Cloning and expression of the Vitreoscilla hemoglobin gene in Burkholderia sp. strain DNT for enhancement of 2,4-dinitrotoluene degradation. Biotechnol Prog 16:26-30
20. Ramandeep, Hwang KW, Raje M, Kim KJ, Stark BC, Dikshit KL, Webster DA (2001) Vitreoscilla hemoglobin: intracellular localization and binding to membranes. J Biol Chem 276:24781-24789
21. Spanggord RJ, Spain JC, Nishino SF, Mortelmans KE (1991) Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp. Appl Environ Microbiol 57:3200-3205
22. Stark BC, Webster DA, Dikshit KL (1999) Vitreoscilla hemoglobin: molecular biology, biochemistry, and practical applications. Recent Res Dev Biotechnol Bioeng 2:155-174
23. Stevanin TM, Ioannidis N, Mills CE, Kim SO, Hughes MN, Poole RK (2000) Flavohemoglobin Hmp affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo or bd, from nitric oxide. J Biol Chem 275:35868-35875
24. Tarricone C, Galizzi A, Coda P, Ascenzi P, Bolognesi M (1997) Unusual structure of the oxygen-binding site in the dimeric bacterial hemoglobin from Vitreoscilla sp. Structure 5:497-507
25. Tsai PS, Hatzimanikatis V, Bailey JE (1996) Effect of Vitreoscilla hemoglobin dosage on microaerobic Escherichia coli carbon and energy metabolism. Biotechnol Bioeng 49:139-150
26. Urgun-Demirtas M, Pagilla KR, Stark BC, Webster DA (2003) Biodegradation of 2-chlorobenzoate by recombinant Burkholderia cepacia expressing Vitreoscilla hemoglobin under variable levels of oxygen availability. Biodegradation 14:357-365
27. Urgun-Demirtas M, Pagilla KR, Stark BC (2005) 2-Chlorobenzoate biodegradation by recombinant Burkholderia cepacia under hypoxic conditions in a membrane bioreactor. Water Environ Res (in press)
28. Wakabayashi S, Matsubara H, Webster DA (1986) Primary sequence of a dimeric bacterial haemoglobin from Vitreoscilla. Nature 322:481-483
29. Webster DA (1987) Structure and function of bacterial hemoglobin and related proteins. In: Eichhorn GL, Marzilli LG (eds) Advances in inorganic biochemistry Heme proteins, vol 7. Elsevier, New York, pp 245-265