Biodegradation of 2-chlorobenzoate by recombinant Burkholderia cepacia expressing Vitreoscilla hemoglobin under variable levels of oxygen availability

Biodegradation

Volumn 14, Issue 5, 2003, Pages 357-365

  Urgun Demirtas, Meltem ^a   Pagilla, Krishna R ^a   Stark, Benjamin C ^a   Webster, Dale ^a  

^a NONE

Author keywords

2 chlorobenzoate; Bacterial hemoglobin; Biodegradation; Burkholderia; Hypoxic

Indexed keywords

BACTERIA; BIOREACTORS; DECHLORINATION; HEMOGLOBIN; OXYGEN; STOICHIOMETRY;

MOLAR RATIO;

BIODEGRADATION;

2 CHLOROBENZOATE; CHLORIDE; CHLOROBENZOIC ACID DERIVATIVE; DISSOLVED OXYGEN; HEMOGLOBIN; OXYGEN; UNCLASSIFIED DRUG;

AROMATIC HYDROCARBON; BIODEGRADATION; DISSOLVED OXYGEN; MICROBIAL ACTIVITY;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIODEGRADATION; BIOREACTOR; BIOREMEDIATION; BURKHOLDERIA CEPACIA; CONTINUOUS CULTURE; DECHLORINATION; DEGRADATION; ECOSYSTEM; HYPOXIA; NONHUMAN; OXYGEN CONSUMPTION; PLASMID; PLASMID PSC160; PROTEIN EXPRESSION; STOICHIOMETRY; VGB GENE; VITREOSCILLA;

BACTERIA (MICROORGANISMS); BURKHOLDERIA; BURKHOLDERIA CEPACIA; BURKHOLDERIA SP.; VITREOSCILLA;

EID: 0141782449     PISSN: 09239820     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1025672528291     Document Type: Article

References (46)

1. Arensdorf JJ & Focht DD (1994) Formation of chlorocatechol meta cleavage products by a Pseudomonad during metabolism of monochlorbiphenyls. Appl. Environ. Microbiol. 60: 2884-2889
2. Arensdorf JJ & Focht DD (1995) A meta cleavage pathway for 4-Chlorbenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166. Appl. Environ. Microbiol. 61: 443-447
3. Balajee S & Mahadevan A (1990) Utilization of chloroaromatic substances by Azotobacter chroococcum. Syst. Appl. Microbiol. 13: 194-198
4. Bartels I, Knackmuss HJ & Reineke W (1984) Suicide inactivation of catechol 2,3-dioxygenase from Pseudomonas putida mt-2 by 3-halocatechols. Appl. Environ. Microbiol. 47: 500-505
5. Champagne P, Van Geel, PJ & Parker WJ (1998) A proposed transient model for cometabolism in biofilm systems. Biotechnol. Bioeng. 60: 541-550
6. Chung JW, Webster DA, Pagilla KR & Stark BC (2001) Chromosomal integration of the Vitreoscilla hemoglobin gene in Burkholderia and Pseudomonas for the purpose of producing stable engineered strains with enhanced bioremediating ability. J. Ind. Microbiol. Biotechnol. 27: 27-33
7. Deweerd KA & Bedard DL (1999) Use of halogenated benzoates and other halogenated aromatic compounds to stimulate the microbial dechlorination of PCBs. Environ. Sci. Technol. 33: 2057-2063
8. Dikshit KL & Webster DA (1988) Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli. Gene 70: 377-386
9. Dikshit KL, Dikshit, RP & Webster DA (1990) Study of Vitreoscilla globin (vgb) gene expression and promoter activity in E. coli through transcriptional fusion. Nucl. Acid Res. 18: 4149-4155
10. Fish PA, Webster DA & Stark BC (2000) Vitroeoscilla hemoglobin enhances the first step in 2,4-dinitrotoluene degradation in vitro and at low aeration in vivo. J. Mol. Cat. B 9: 75-82
11. Flanagan WP & May RJ (1993) Metabolite detection as evidence for naturally occurring aerobic PCB biodegradation in Hudson River sediments. Environ. Sci. Technol. 27: 2207-2212
12. Harkness MR, McDermott JB, Abramowicz DA, Salvo JJ, Flanagan WP, Stephens ML, Mondello FJ, May RJ, Lobos JH, Carroll KM, Brenna MJ, Bracco AA, Fish KM, Warner GL, Wilson PR, Dietrich DK, Lin DT, Morgan, CB & Gately WL (1993) In situ stimulation of aerobic PCB degradation in Hudson river sediments. Science 259: 503-507
13. Joshi M & Dikshit KL (1994) Oxygen dependent regulation of Vitreoscilla globin gene: evidence for positive regulation by FNR. Biochem. Biophys. Com. 202: 535-542
14. Khosla C & Bailey JE (1988a) The Vitreoscilla hemoglobin gene: Molecular cloning, nucleotide sequence, and genetic expression in Escherichia coli. Mol. Gen. Genetics. 214: 158-161
15. Khosla C & Bailey JE (1988b) Heterologous expression of a bacterial hemoglobin improves the growth properties of recombinant Escherichia coli. Nature 331: 633-635
16. Kröckel L & Focht DD (1987) Construction of chlorobenzene utilizing recombinants by progentive manifestation of a rare event. Appl. Environ. Microbiol. 53: 2470-2475
17. Krooneman J, Wieringa EBA, Moore ERB, Gerritse J, Prins RA & Gottschal, JC (1996) Isolation of Alcaligenes sp. strain L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (chloro)catechols. Appl. Environ. Microbiol. 62: 2427-2434
18. Krooneman J, Moore ERB, van Velzen JCL, Prins RA, Forney, LJ & Gottschal, JC (1999) Isolation of Alcaligens sp. train L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (Chloro) catechols. Appl. Environ. Microbiol. 62: 2427-2434
19. Lin JM, Stark BC & Webster DA (in press) Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (DNT) dioxygenase activity of Burkholderia and on DNT degradation in two-phase bioreactors. J. Ind. Microbiol. Biotechnol.
20. Liu SC, Webster DA & Stark BC (1995) Cloning and expression of Vitreoscilla hemoglobin gene in Pseudomonas: Effects on cell growth. Appl. Microbiol. Biotechnol. 44: 419-424
21. Mars AE, Kasberg T, Kaschabek SR, van Agteren MH, Janssen DB & Reineke W (1997) Microbial degradation of chloroaromatics: use of the meta-cleavage pathway for mineralization of chlorobenzene. J. Bacteriol. 179: 4530-4537
22. Müller R, Deckwer WD & Hecht V (1996) Degradation of chloro- and methyl- substituted benzoic acids by a genetically modified microorganism. Biotechnol. Bioeng. 51: 528-537
23. Nasr MA, Hwang KW, Akbas M, Webster DA & Stark BC (2001) Effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by Burkholderia engineered with the Vitreoscilla hemoglobin gene. Biotechnol. Prog. 17: 359-361
24. Niedan V & Schöler HF (1997) Natural formation of chlorobenzoic acids (CBA) and distinction between PCB degraded bacteria. Chemosphere 35: 1233-1241
25. Oldenhius R, Kuijk L, Lammers A, Janssen DB & Witholt B (1989) Degradation of chlorinated and nonchlorinated aromatic solvents in soil suspensions by pure bacterial cultures. Appl. Microbiol. Biotechnol. 30: 211-217
26. Oltmanns HG, Rast RH & Reineke W (1988) degradation of 1,4-dichlorobenzene and toluene by a Pseudomonas strain. Appl. Environ. Microbiol. 57: 157-162
27. Park KW, Kim KJ, Howard AJ, Stark BC & Webster DA (2002) Vitreoscilla hemoglobin binds to subunit I of cytochrome bo ubiquinol oxidases. J. Biol. Chem. 277: 33334-33337
28. Patel SM, Stark BC, Hwang KW, Dikshit KL & Webster DA (2000) Cloning and expression of Vitreoscilla hemoglobin gene in Burkholderia sp. strain DNT for enhancement of 2,4-dinitrotoluene degradation. Biotechnol. Prog. 16: 26-30
29. Rainer, BW (1990) Oxygen transfer in bioreactors. Chem. Biochem Eng. Q4. 4: 185-196
30. Ramandeep, Hwang KW, Raje M, Kim KJ, Stark BC, Dikshit KL & Webster DA (2001) Vitreoscilla hemoglobin: Intracellular localization and binding to membranes. J. Biol. Chem. 276: 24781-24789
31. Reineke W & Knackmuss HJ (1984) Microbial metabolism of haloaromatics: Isolation and properties of a chlorobenzene degrading bacterium. Appl. Environ. Microbiol. 47: 395-402
32. Romanov V & Hausinger RP (1994) Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate. J. Bacteriol. 176: 3368-3374
33. Schmidt E & Knackmuss H J (1980) Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem. J. 192: 339-347
34. Seeger M, Zielinski M, Timmis KN & Hofer B (1999) Regio-specifity of dioxygenation of di- to pentachlorobiphenyls and their degradation to chlorobenzoates by the bph-encoded catabolic pathway of Burkholderia sp. strain LB400. Appl. Environ. Microbiol. 65: 3614-3621
35. Spanggord RJ, Spain JC, Nishino SF & Mortelmans KE (1991) Biodegradation of 2,4-dinitrotoulene by a Pseudomonas sp. Appl. Environ. Microbiol. 57: 3200-3205
36. Tross ME, Schraa G & Zehinder AJB (1996) Transformation of low concentrations of 3-chlorobenzoate by Pseudomonas sp. strain B13: Kinetics and residual concentrations. Appl. Environ. Microbiol. 62: 437-442
37. Tsai PS, Hatzimanikatis V & Bailey JE (1996) Effect of Vitreoscilla hemoglobin dosage on microaerobic Escherchia coli carbon and energy metabolism. Biotechnol. Bioeng. 49: 139-150
38. Tsoi TV, Plotnikova EG, Cole J, Guerin WF, Bagdasarian M & Tiedje J (1999) Cloning, expression and nucleotide sequence of the Pseudomonas aeruginosa 142 ohb genes coding for oxygenolytic ortho dehalogenation of halobenzoates. Appl. Environ. Microbiol. 65: 2151-2162
39. Van Der Woude BJ (1996) Extent of reductive dechlorination of chlorobenzoates in anoxic sediment slurries depends on the sequence of chlorine removal. Environ. Science Technol. 30: 1352-1357
40. Van Der Woude BJ, Gottschal JC & Prins RA (1995) Degradation of 2,5-dichlorobenzoic acid by Pseudomonas aeruginosa JB2 at low oxygen tensions. Biodegradation 6: 39-46
41. Viliesid F & Lilly MD (1992) Influence of dissolved oxygen tension on the synthesis of catechol 1,2-dioxygenase by Pseudomonas putida. Enzyme Microbiol. Technol. 14: 561-565
42. Vollmer MD & Schlomann M (1995) Conversion of 2-chloro-cis,cis-muconate and its metabolites 2-chloro- and 5-chloromuconolactone by chloromuconate cycloisomerases of pJP4 and pAC27. J. Bacteriol. 177: 2938-2941
43. Wakabayashi S, Matsubara H & Webster DA (1986) Primary sequence of a dimeric bacterial hemoglobin from Vitreoscilla. Nature 322: 481-483
44. Webster D (1987) Structure and function of bacterial hemoglobin and related proteins. In: Eichorn GC & Marzilli LG (Eds) Advances in Inorganic Biochemistry, Vol 7 (pp 245-265). Elsevier, New York
45. Zaitsev GM, Uotila JS, Tsitko IV, Lobanok AG & Salonen MSS (1995) Utilization of halogenated benzenes, phenols and benzoates by Rhodococcus opasus GM-14. Appl. Environ. Microbiol. 61: 4191-4201
46. Zaitsev GM & Karasevich YN (1984) Utilization of 2-chlorobenzoic acid by Pseudomonas cepacia. Mikrobiologiya 53: 75-80