Chimeric Vitreoscilla hemoglobin (VHb) carrying a flavoreductase domain relieves nitrosative stress in Escherichia coli: New insight into the functional role of VHb

Applied and Environmental Microbiology

Volumn 68, Issue 1, 2002, Pages 152-160

  Kaur, R ^a   Pathania, R ^a   Sharma, V ^a   Mande, S C ^a   Dikshit, K L ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENZYMES; GEL PERMEATION CHROMATOGRAPHY; METABOLISM; MONOMERS; PHYSIOLOGY; SODIUM COMPOUNDS;

DIMERIC HEMOGLOBIN;

ESCHERICHIA COLI;

CHIMERIC PROTEIN; DIMER; FLAVOREDUCTASE; HEMOGLOBIN; MONOMER; NITRIC OXIDE; NITROPRUSSIDE SODIUM; OXIDOREDUCTASE; OXYGEN; UNCLASSIFIED DRUG; VITREOSCILLA HEMOGLOBIN;

BACTERIUM;

ARTICLE; BACTERIAL MUTATION; BACTERIUM MUTANT; CELL RESPIRATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISSOCIATION; ENVIRONMENTAL FACTOR; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE OVEREXPRESSION; NITROSATION; NONHUMAN; OXYGEN TRANSPORT; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRESS; STRUCTURE ANALYSIS; VITREOSCILLA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; ESCHERICHIA COLI; FMN REDUCTASE; GENE DELETION; HEAT-SHOCK RESPONSE; HEMOGLOBINS; MOLECULAR SEQUENCE DATA; NADH, NADPH OXIDOREDUCTASES; NITRIC OXIDE; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; VITREOSCILLA;

ALCALIGENES; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RALSTONIA; VITREOSCILLA; VITREOSCILLA SP.; WAUTERSIA EUTROPHA;

EID: 0036135136     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.1.152-160.2002     Document Type: Article

References (45)

1. The protein data bank
2. Control of heme content in Vitreoscilla by oxygen
3. Flavohemoglobin expression and function in Saccharomyces cerevisiae. No relationship with respiration and complex response to oxidative stress
4. Primary sequence and evidence for a physiological function of the flavohemoprotein of Alcaligenes eutrophus
5. Regulation of Saccharomyces cervisiae flavohemoglobin expression
6. Regulation of the Salmonella typhimurium flavohemoglobin gene. A new pathway for bacterial gene expression in response to nitric oxide
7. Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli
8. Oxygen inhibition of globin gene transcription and bacterial haemoglobin synthesis in Vitreoscilla
9. Study of Vitreoscilla globin (vgb) gene expression and promoter activity in E. coli through transcriptional fusion
10. The bacterial hemoglobin from Vitreoscilla can support the aerobic growth of Eschelichia coli lacking terminal oxidases
11. Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 Å resolution
12. Flavohemoprotein HMPX: A new pathogenicity determinant in Erwinia crysanthemi strain 3937
13. Steady state and transient kinetics of Escherichia coli nitric oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysis
14. Nitric oxide dioxygenase: An enzymic function for flavohemoglobin
15. Purification and properties of NADH-cytochrome o reductase from Vitreoscilla
16. The evolution of hemoglobin
17. Nitrosative stress: Metabolic pathway involving the flavohemoglobin
18. Transgenic tobacco expressing Vilreoscilla hemoglobin exhibits enhanced growth and altered metabolite production
19. NACCESS computer program
20. Identification and characterization of two flavohemoglobin genes in Dictyostelium discoideum
21. NADH-dependent methmoglobin reductase from the obligate aerobe Vitreoscilla: Improved method of purification and reexamination of prosthetic groups
22. Surface, subunit interface, and interior of oligomeric proteins
23. Improved methods for building protein models in electron-density maps and the location of errors in these models
24. Hemoglobin biosynthesis in Vitreoscilla stercoraria DW: Cloning, expression, and characterization of a new homolog of a bacterial globin gene
25. Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli
26. Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli
27. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli
28. Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain
29. Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis
30. Protection from nitrosative stress by yeast flavohemoglobin
31. The flavohemoglobin of Escherichia coli confers resistance to a nitrosating agent, a "nitric oxide releaser" and paraquat and is essential for transcriptional responses to oxidative stress
32. Structural analysis of monomeric: Hemichrime and dimeric cyanomet hemoglobins from Caudina arenicola
33. Effect of Vitreoscilla hemoglobin expression in growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells
34. Escherichia coli flavohemoglobin (HMP) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide
35. Nitric oxide, nitrite and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12
36. New functions for the ancient globin family: Bacterial responses to nitric oxide and nitrosative stress
37. The Vitreoscillaceae: A family of colourless, gliding, filamentous organisms
38. An oxygen binding flavohemoprotein from Alcaligenes eutrophus
39. Vitreoscilla hemoglobin: Intracellular localization and binding to membranes
40. Flavohemoglobin HMP affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo' or bd' from nitric oxide
41. Unusual structure of the oxygen binding site in the dimeric bacterial hemoglobin from Vitreoseilla sp.
42. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice
43. Isolation and nucleotide sequence of hmp gene that encodes a hemoglobin-like protein in E. coli K-12
44. Primary sequence of a dimeric bacterial hemoglobin from Vitreoscilla
45. Structure and function of bacterial hemoglobin and related proteins