Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (2,4-DNT) dioxygenase activity of Burkholderia and on 2,4-DNT degradation in two-phase bioreactors

Journal of Industrial Microbiology and Biotechnology

Volumn 30, Issue 6, 2003, Pages 362-368

  Lin, Jui Ming ^a   Stark, Benjamin C ^a   Webster, Dale A ^a  

^a Illinois Institute of Technology   (United States)

Author keywords

2,4 Dinitrotoluene; 2,4 DNT biodegradation; Hemoglobin; Two phase bioreactors

Indexed keywords

2,4 DINITROTOLUENE; 2,4 DINITROTOLUENE DIOXYGENASE; HEMOGLOBIN; OLEYL ALCOHOL; OXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BIODEGRADATION; BIOREACTOR; BURKHOLDERIA; CELL GROWTH; ENZYME ACTIVITY; FED BATCH REACTOR; GENE EXPRESSION; HYPOXIA; NONHUMAN; OXYGEN CONSUMPTION; OXYGEN TRANSPORT; STRAIN DIFFERENCE; VITREOSCILLA;

BACTERIAL PROTEINS; BIODEGRADATION, ENVIRONMENTAL; BIOREACTORS; BURKHOLDERIA; CYTOSOL; ENZYME ACTIVATION; FATTY ALCOHOLS; HEMOGLOBINS; OXYGEN; OXYGENASES;

BACTERIA (MICROORGANISMS); BOVINAE; BURKHOLDERIA; NEGIBACTERIA; VITREOSCILLA;

EID: 0041375628     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10295-003-0054-0     Document Type: Article

References (44)

1. Aydin S, Webster DA, Stark BC (2000) Nitrite inhibition of Vitreoscilla hemoglobin (VHb) in recombinant E. coli: direct evidence that VHb enhances recombinant protein production. Biotechnol Prog 16:917-921
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
3. 2. Biotechnol Lett 18:695-700
4. Chung JW, Webster DA, Pagilla KR, Stark BC (2001) Chromosomal integration of the Vitreoscilla hemoglobin gene in Burkholderia and Pseudomonas for the purpose of producing stable engineered strains with enhanced bioremediating ability. J Ind Microbiol Biotechnol 27:27-33
5. Collins LD, Daugulis AJ (1996) Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors. Biotechnol Bioeng 55:155-162
6. Collins LD, Daugulis AJ (1999) Simultaneous biodegradation of benzene, toluene, and p-xylene in a two-phase partitioning bioreactor: concept demonstration and practical application. Biotechnol Prog 15:74-80
7. DeModena JA, Gutierrez S, Velasco J, Fernandez FJ, Fachini RA, Galazzo JL, Hughes DE, Martin JF (1993) The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin. Bio/Technology 11:926-929
8. Dikshit KL, Webster DA (1988) Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli. Gene 70:377-386
9. Dikshit KL, Spaulding D, Braun A, Webster DA (1989) Oxygen inhibition of globin gene transcription and bacterial haemoglobin synthesis in Vitreoscilla. J Gen Microbiol 135:2601-2609
10. Dikshit RP, Dikshit KL, Liu Y, Webster DA (1992) The bacterial hemoglobin from Vitreoscilla can support the aerobic growth of Escherichia coli lacking terminal oxidases. Arch Biochem Biophys 293:241-245
11. - recombinant Escherichia coli: some observations. Biotechnol Prog 15:640-645
12. Fish PA, Webster DA, Stark BC (2000) Vitreoscilla hemoglobin enhances the first step in 2,4-dinitrotoluene degradation in vitro and at low aeration in vivo. J Mol Catal B Enzym 9:75-82
13. Georgiou CD, Webster DA (1987) Identification of b, c, and d cytochromes in the membrane of Vitreoscilla. Arch Microbiol 148:328-333
14. Haigler BE, Suen WC, Spain JC (1996) Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT. J Bacteriol 178:6019-6024
15. Holmberg N, Lilius G, Bailey JE, Bülow L (1997) Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolic production. Nat Botechnol 15:244-247
16. Kallio PT, Bailey JE (1996) Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of alpha-amylase and neutral protease in Bacillus subtilis. Biotechnol Prog 12:31-39
17. Kallio PT, Kim DJ, Tsai PS, Bailey JE (1994) Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. Eur J Biochem 219:201-208
18. Kallio PT, Tsai PS, Bailey JE (1996) Expression of Vitreoscilla hemoglobin is superior to horse heart myoglobin or yeast flavohemoglobin expression for enhancing Excherichia coli growth in a microaerobic bioreactor. Biotechnol Prog 6:751-757
19. Keith LH, Telliard WA (1979) Priority pollutants. I. A perspective view. Environ Sci Technol 13:416-423
20. Khosla C, Bailey JE (1988a) Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli. Nature 331:633-635
21. Khosla C, Bailey JE (1988b) The Vitreoscilla hemoglobin gene: molecular cloning, nucleotide sequence, and genetic expression in Escherichia coli. Mol Gen Genet 214:158-161
22. Khosravi M, Webster DA, Stark BC (1990) Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain. Plasmid 24:190-194
23. Kieboom J, Dennis JJ, Bont JAM de, Zylstra GJ (1998) Identification and molecular characterization of an efflux pump involved in Pseudomonas putida S12 solvent tolerance. J Biol Chem 273:85-91
24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
25. Liu SC, Webster DA, Stark BC (1995) Cloning and expression of the Vitreoscilla hemoglobin gene in Pseudomonas: effects on cell growth. Appl Microbiol Biotechnol 44:419-424
26. Liu SC, Webster DA, Wei ML, Stark BC (1996) Genetic engineering to contain the Vitreoscilla hemoglobin gene enhances degradation of benzoic acid by Xanthomonas maltophilia. Biotechnol Bioeng 49:101-105
27. Magnolo SK, Leenutaphong DL, DeModena JA, Curtis JE, Bailey JE, Galazzo JL, Hughes DE (1991) Actinorhodin production by Streptomyces coelicolor and growth of Streptomyces lividans are improved by the expression of a bacterial hemoglobin. Bio/Technology 9:473-476
28. Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117:307-310
29. Nasr MA, Hwang KW, Akbas M, Webster DA, Stark BC (2000) Effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by Burkholderia engineered with the Vitreoscilla hemoglobin gene. Biotechnol Prog 17:359-361
30. Park KW, Kim KJ, Howard AJ, Stark BC, Webster DA (2002) Vitreoscilla hemoglobin binds to subunit I of cytochrome bo ubiquinol oxidases. J Biol Chem 277:33334-33337
31. Patel SM, Stark BC, Hwang KW, Dikshit KL, Webster DA (2000) Cloning and Vitreoscilla hemoglobin gene in Burkholderia sp. strain DNT for enhancement of 2,4-dinitrotoluene degradation. Biotechnol Prog 16:26-30
32. Pendse GJ, Bailey JE (1994) Effects of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells. Biotechnol Bioeng 44:1367-1370
33. Pringsheim EG (1951) The Vitreoscillaceae: a family of colourless, gliding, filamentous organisms. J Gen Microbiol 5:124-149
34. Ramandeep, Hwang KW, Raje M, Kim KJ, Stark BC, Dikshit KL, Webster DA (2001) Vitreoscilla hemoglobin. Interacellullar localization and binding to membranes. J Biol Chem 27:24781-24789
35. Spanggord RJ, Spain JC, Nishino SF, Mortelmans KE (1991) Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp. Appl Environ Microbiol 57:3200-3205
36. Suen WC, Spain JC (1993) Cloning and characterization of Pseudomonas sp. DNT genes for 2,4-dinitrotoluene degradation. J Bacteriol 175:1831-1837
37. Suen WC, Haigler BE, Spain JC (1996) 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase. J Bacteriol 178:4926-4934
38. Switzer RL, Garrity LF (1999) Experimental biochemistry. Julet, New York
39. - recombinant Escherichia coli: a comparative study. Biotechnol Bioeng 59:673-678
40. Tarricone C, Galizzi A, Coda A, Ascenzi P, Bolognesi M (1997) Unusual structure of the oxygen-binding site in the dimeric bacterial hemoglobin from Vitreoscilla sp. Structure 5:497-507
41. Wakabayashi S, Matsubara H, Webster DA (1986) Primary sequence of a dimeric bacterial haemoglobin from Vitreoscilla. Nature 322:481-483
42. Webster DA (1987) Structure and function of bacterial hemoglobin and related proteins. In: Eichhorn GC, Marzilli LG (eds) Advances in inorganic biochemistry. Elsevier, New York, pp 245-265
43. Wei ML, Webster DA, Stark BC (1998) Genetic engineering of Serratia marcescens with bacterial hemoglobin gene: effects on growth, oxygen utilization, and cell size. Biotechnol Bioeng 57:477-483
44. Wei ML, Webster DA, Stark BC (1998) Metabolic engineering of Serratia marcescens with the bacterial hemoglobin gene: alterations in fermentation pathways. Biotechnol Bioeng 59:640-646