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21
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26
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0037425124
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Very recently, Niemeyer and co-workers reported the synthesis of PNA-protein conjugates using EPL. However, the synthesis of DNA-protein conjugates by EPL has not yet been reported.
-
Very recently, Niemeyer and co-workers reported the synthesis of PNA-protein conjugates using EPL. However, the synthesis of DNA-protein conjugates by EPL has not yet been reported. Lovrinovic M., Seidel R., Wacker R., Schroeder H., Seitz O., Engelhard M., Goody R.S., Niemeyer C.M. Chem. Commun. 2003;822.
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Niemeyer, C.M.8
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28
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1942492431
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-
note
-
+=662.20, obsd 662.00.
-
-
-
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29
-
-
1942492459
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-
note
-
HPLC was carried out on a μBondasphere C18 column (5 μ, 150 × 3.8 mm, Waters) eluted with 0.1 M ammonium acetate buffer (pH 7.0) containing 0-90% acetonitrile in a linear gradient over 60 min at a flow rate of 1 mL/min, detected at 260 nm.
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-
-
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30
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-
1942460014
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-
note
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+=3982.7, obsd. 3982.8.
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-
-
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31
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0032988320
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Southworth M.W., Amaya K., Evans T.C., Xu M.Q., Perler F.B. BioTechniques. 27:1999;110.
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Southworth, M.W.1
Amaya, K.2
Evans, T.C.3
Xu, M.Q.4
Perler, F.B.5
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32
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-
1942524456
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-
note
-
All reactions were carried out in a total volume of 10 μL of buffer B (50 mM Tris-HCl, 500 mM NaCl, 1 mM EDTA, 50 mM MESNA, pH 8.5). Final concentrations of ECFP and 3-ODNs were 13 and 100 μM, respectively.
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-
-
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33
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-
1942428155
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note
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All samples were boiled in a loading buffer containing 2-mercaptoethanol to completely cleave disulfide bonds prior to SDS-PAGE analysis.
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-
-
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34
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0042526048
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+=30975.04, obsd 30955.0. DTT can be used after the ligation step to reduce any unwanted disulfide bonds.
-
+=30975.04, obsd 30955.0. DTT can be used after the ligation step to reduce any unwanted disulfide bonds. Nyanguile O., Dancik C., Blackmore J., Mulgrew K., Kaleko M., Stevenson S.C. Gene Therapy. 10:2003;1362.
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(2003)
Gene Therapy
, vol.10
, pp. 1362
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Nyanguile, O.1
Dancik, C.2
Blackmore, J.3
Mulgrew, K.4
Kaleko, M.5
Stevenson, S.C.6
|