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Volumn 14, Issue 10, 2004, Pages 2407-2410

Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

Author keywords

Cysteine modification; Expressed protein ligation; Protein DNA conjugate

Indexed keywords

CYSTEINE; HYBRID PROTEIN; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN;

EID: 1942438549     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.03.023     Document Type: Article
Times cited : (40)

References (34)
  • 26
    • 0037425124 scopus 로고    scopus 로고
    • Very recently, Niemeyer and co-workers reported the synthesis of PNA-protein conjugates using EPL. However, the synthesis of DNA-protein conjugates by EPL has not yet been reported.
    • Very recently, Niemeyer and co-workers reported the synthesis of PNA-protein conjugates using EPL. However, the synthesis of DNA-protein conjugates by EPL has not yet been reported. Lovrinovic M., Seidel R., Wacker R., Schroeder H., Seitz O., Engelhard M., Goody R.S., Niemeyer C.M. Chem. Commun. 2003;822.
    • (2003) Chem. Commun. , pp. 822
    • Lovrinovic, M.1    Seidel, R.2    Wacker, R.3    Schroeder, H.4    Seitz, O.5    Engelhard, M.6    Goody, R.S.7    Niemeyer, C.M.8
  • 28
    • 1942492431 scopus 로고    scopus 로고
    • note
    • +=662.20, obsd 662.00.
  • 29
    • 1942492459 scopus 로고    scopus 로고
    • note
    • HPLC was carried out on a μBondasphere C18 column (5 μ, 150 × 3.8 mm, Waters) eluted with 0.1 M ammonium acetate buffer (pH 7.0) containing 0-90% acetonitrile in a linear gradient over 60 min at a flow rate of 1 mL/min, detected at 260 nm.
  • 30
    • 1942460014 scopus 로고    scopus 로고
    • note
    • +=3982.7, obsd. 3982.8.
  • 32
    • 1942524456 scopus 로고    scopus 로고
    • note
    • All reactions were carried out in a total volume of 10 μL of buffer B (50 mM Tris-HCl, 500 mM NaCl, 1 mM EDTA, 50 mM MESNA, pH 8.5). Final concentrations of ECFP and 3-ODNs were 13 and 100 μM, respectively.
  • 33
    • 1942428155 scopus 로고    scopus 로고
    • note
    • All samples were boiled in a loading buffer containing 2-mercaptoethanol to completely cleave disulfide bonds prior to SDS-PAGE analysis.
  • 34
    • 0042526048 scopus 로고    scopus 로고
    • +=30975.04, obsd 30955.0. DTT can be used after the ligation step to reduce any unwanted disulfide bonds.
    • +=30975.04, obsd 30955.0. DTT can be used after the ligation step to reduce any unwanted disulfide bonds. Nyanguile O., Dancik C., Blackmore J., Mulgrew K., Kaleko M., Stevenson S.C. Gene Therapy. 10:2003;1362.
    • (2003) Gene Therapy , vol.10 , pp. 1362
    • Nyanguile, O.1    Dancik, C.2    Blackmore, J.3    Mulgrew, K.4    Kaleko, M.5    Stevenson, S.C.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.