A convenient solid-phase method for synthesis of 3′-conjugates of oligonucleotides

Bioconjugate Chemistry

Volumn 12, Issue 4, 2001, Pages 576-586

  Stetsenko, D A ^a   Gait, M J ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCONJUGATE; HOMOSERINE; HYDROXYL GROUP; OLIGONUCLEOTIDE; PIPERIDINE; REACTION MECHANISM; SOLID PHASE; SYNTHESIS;

AMMONIA; MOLECULES; PEPTIDES; SYNTHESIS (CHEMICAL);

BRANCHING UNITS; COUPLING REACTION; DEPROTECTION; HOMOSERINE; HYDROXYL GROUPS; PROTECTING GROUP; SOLID PHASE SYNTHESIS; SOLID SUPPORTS; SOLID-PHASE METHOD; Α-POSITIONS;

OLIGONUCLEOTIDES;

EID: 0034918592     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc000157g     Document Type: Article

References (55)

1. Efficient methods for attaching nonradioactive labels to the 5′-ends of synthetic oligodeoxyribonucleotides
2. Stepwise solid-phase synthesis of peptide-oligonucleotide conjugates on new solid supports
3. Inhibition of the HIV-1 tat protein-TAR RNA interaction by 2′-O-methyl oligoribonucleotides
4. Solid-phase synthesis of modified oligodeoxyribonucleotides with an acridine derivative or a thiophosphate group at their 3′-end
5. New solid-phase for automated synthesis of oligonucleotides containing an amino-alkyl linker at their 3′-end
6. Optimized synthesis of phosphorothioate oligodeoxyribonucleotides substituted with a 5′-protected thiol and a 3′-amino function
7. Stepwise solid-phase synthesis of serine-, tyrosine- and homoserine-nucleopeptides
8. Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides
9. Solid-phase synthesis of 5′,3′-bifunctional oligodeoxyribonucleotides bearing a masked thiol group at the 3′-end
10. Modern machine-aided methods of oligodeoxyribonucleotide synthesis
11. A new solid-phase synthesis of oligonucleotides 3′-conjugated with peptides
12. Site-specific cross-linking of amino acids in the basic region of Human Immunodeficiency Virus type 1 Tat peptide to chemically modified TAR RNA duplexes
13. Synthesis of N-substituted glycine peptoid libraries
14. Facile preparation of nuclease resistant 3′-modified oligodeoxynucleotides
15. A general method for the synthesis of 3′-sulfhydryl and phosphate group containing oligonucleotides
16. A convenient synthetic route to oligonucleotide conjugates
17. Synthesis and properties of oligodeoxyribonucleotide-poly(ethylene glycol) conjugates
18. Water-soluble block polycations as carriers for oligonucleotide delivery
19. Solution-phase bioconjugate synthesis using protected oligonucleotides containing 3′-alkyl carboxylic acids
20. High-yielding method for on-column derivatization of protected oligodeoxynucleotides and its application to the convergent synthesis of 5′,3′-bis-conjugates
21. Delivery of antisense oligonucleotides and plasmid DNA with various carrier agents
22. A pyrene secopseudonucleoside in constructing interaction-sensitive fluorescent DNA probes
23. Modification of antisense phosphodiester oligodeoxynucleotides by a 5′-cholesteryl moiety increases cellular association and improves efficacy
24. Cholesteryl-conjugated oligonucleotides: Synthesis, properties and activity as inhibitors of replication of human immunodeficiency virus in cell culture
25. Hybridization properties of deoxyoligonucleotides containing anthraquinone pseudonucleosides
26. Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans-activation responsive region (TAR) of HIV RNA
27. Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides
28. Automated synthesis of double dye-labeled oligonucleotides using tetramethylrhodamine (TAMRA) solid supports
29. Bifunctional oligonucleotide probes synthesized using a novel CPG support are able to detect single base pair mutations
30. A new and versatile reagent for incorporating multiple primary aliphatic amines into synthetic oligonucleotides
31. Oligonucleotide labeling methods 3. Direct labeling of oligonucleotides emplying a novel, nonnucleosidic, 2-aminobutyl-1, 3-propanediol backbone
32. An improved CPG support for the synthesis of 3′-amine-tailed oligonucleotides
33. 3′-End conjugates of minimally phosphorothioate-protected oligonucleotides with 1-O-hexadecylglycerol: Synthesis and anti-ras activity on radiation-resistant cells
34. Acridine- and cholesterol-derivatized solid supports for improved synthesis of 3′-modified oligonucleotides
35. A novel synthesis of oligonucleotide-peptide conjugates with a base-labile phosphate linker between the two components according to the allyl-protected phosphoramidite strategy
36. Synthesis of 3′,5-dipeptidyl oligonucleotides
37. Synthesis, hybridization properties and antiviral activity of lipid-oligodeoxynucleotide conjugates
38. Antisense oligodeoxynucleotides: Internalization, compartmentalization and nonsequence specificity
39. Peptide conjugates of oligonucleotides as enhanced antisense agents: A review
40. Efficient conjugation of peptides to oligonucleotides by "native ligation"
41. New phosphoramidite reagents for the synthesis of oligonucleotides containing a cysteine residue useful in peptide conjugation
42. Fluorescent dye phosphoramidite labeling of oligonucleotides
43. Preparation and application of peptide-oligonucleotide conjugates
44. The synthesis of polyamide nucleic acids using a novel monomethoxytrityl protecting-group strategy
45. Efficient methods for attachment of thiol specific probes to the 3′-ends of synthetic oligodeoxyribonucleotides