Downregulation of c-FLIP sensitizes DU145 prostate cancer cells to Fas-mediated apoptosis

Cancer Biology and Therapy

Volumn 1, Issue 4, 2002, Pages 401-406

  Hyer, Marc L ^a   Sudarshan, Sunil ^a   Kim, Youngsoo ^b   Reed, John C ^b   Dong, Jian Yun ^a   Schwartz, David A ^a   Norris, James S ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b BURNHAM INSTITUTE   (United States)

Author keywords

Apoptosis; c FLIP; Caspases; CD95; CH 11; DISC; Fas ligand; Prostate cancer

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; BID PROTEIN, HUMAN; CARRIER PROTEIN; CASP3 PROTEIN, HUMAN; CASP7 PROTEIN, HUMAN; CASP8 PROTEIN, HUMAN; CASP9 PROTEIN, HUMAN; CASPASE; CASPASE 3; CASPASE 7; CASPASE 8; CASPASE 9; CFLAR PROTEIN, HUMAN; CYTOCHROME C; FAS ANTIGEN; FAS LIGAND; FASLG PROTEIN, HUMAN; FLICE INHIBITORY PROTEIN; MEMBRANE PROTEIN; PROTEIN BID; SIGNAL PEPTIDE;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL LINE; CELL SEPARATION; DOWN REGULATION; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMAN; IMMUNOBLOTTING; LEUKEMIA CELL LINE; MALE; METABOLISM; MITOCHONDRION; PATHOLOGY; PROSTATE TUMOR; SIGNAL TRANSDUCTION; TIME; WESTERN BLOTTING;

ANTIGENS, CD95; APOPTOSIS; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; BLOTTING, WESTERN; CARRIER PROTEINS; CASP8 AND FADD-LIKE APOPTOSIS REGULATING PROTEIN; CASPASE 3; CASPASE 7; CASPASE 8; CASPASE 9; CASPASES; CELL DEATH; CELL LINE; CELL SEPARATION; CYTOCHROME C GROUP; DOWN-REGULATION; ENZYME ACTIVATION; FAS LIGAND PROTEIN; FLOW CYTOMETRY; HUMANS; IMMUNOBLOTTING; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; JURKAT CELLS; MALE; MEMBRANE GLYCOPROTEINS; MITOCHONDRIA; OLIGONUCLEOTIDES, ANTISENSE; PROSTATIC NEOPLASMS; SIGNAL TRANSDUCTION; TIME FACTORS; TUMOR CELLS, CULTURED;

EID: 1842843211     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.1.4.15     Document Type: Article

References (25)

1. DeVita VT, Hellman S, Rosenberg SA. Cancer Principal and Practice of Oncology. 5th edition, Lippincott-Raven, 1997.
2. Konety BR, Getzenberg RH. Novel therapies for advanced prostate cancer. Semin Urol Oncol 1997; 15:33-42. (Pubitemid 27091625)
3. Suzuki A, Matsuzawa A, Iguchi T. Down regulation of Bcl-2 is the first step on Fas-mediated apoptosis of male reproductive tract. Oncogene 1996; 13:31-7. (Pubitemid 26248542)
4. Suda T, Nagata S. Purification and characterization of the Fas-ligand that induces apoptosis. J Exp Med 1994; 179:873-9. (Pubitemid 24065645)
5. Takahashi T, Tanaka M, Inazawa J, Abe T, Suda T, Nagata S. Human Fas ligand: gene structure, chromosomal location and species specificity. Int Immunol 1994; 6(10):1567-74. (Pubitemid 24317866)
6. Ashkenazi A, Dixit VM. Death receptors: signaling and modulation. Science 1998; 281:1305-8. (Pubitemid 28406815)
7. Medema JP, Scaffidi C, Kischkel FC, Shevchenko A, Mann M, Krammer PH, et al. FLICE is activated by association with the CD95 death-inducing signaling complex (DISC). Embo J 1997; 16:2794-804. (Pubitemid 27226216)
8. Muzio M, Salvesen GS, Dixit VM. FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. J Biol Chem 1997; 272:2952-6. (Pubitemid 27053346)
9. Willems F, Amraoui Z, Vanderheyde N, Verhasselt V, Aksoy E, Scaffidi C, et al. Expression of c-FLIP(L) and resistance to CD95-mediated apoptosis of monocyte-derived dendritic cells: inhibition by bisindolylmaleimide. Blood 2000; 95:3478-82. (Pubitemid 30428480)
10. Fulda S, Meyer E, Debatin KM. Metabolic inhibitors sensitize for CD95 (APO-1/Fas)- induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression. Cancer Res 2000; 60:3947-56. (Pubitemid 32204591)
11. Scaffidi C, Schmitz I, Krammer PH, Peter ME. The role of c-FLIP in modulation of CD95-induced apoptosis. J Biol Chem 1999; 274:1541-8.
12. Hyer ML, Voelkel-Johnson C, Rubinchik S, Dong J, Norris JS. Intracellular fas ligand expression causes fas-mediated apoptosis in human prostate cancer cells resistant to monoclonal antibody-induced apoptosis. Mol Ther 2000; 2:348-58.
13. Bennett CF, Chiang MY, Chan H, Shoemaker JE, Mirabelli CK. Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides. Mol Pharmacol 1992; 41:1023-33. (Pubitemid 23026100)
14. McKay RA, Miraglia LJ, Cummins LL, Owens SR, Sasmor H, Dean NM. Characterization of a potent and specific class of antisense oligonucleotide inhibitor of human protein kinase C-alpha expression. J Biol Chem 1999; 274:1715-22.
15. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 1992; 13:204-8.
16. O'Brien MC, Bolton WE. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry 1995; 19:243-55.
17. Muzio M, Stockwell BR, Stennicke HR, Salvesen GS, Dixit VM. An induced proximity model for caspase-8 activation. J Biol Chem 1998; 273:2926-30. (Pubitemid 28133943)
18. Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, et al. Two CD95 (APO- 1/Fas) signaling pathways. Embo J 1998; 17:1675-87. (Pubitemid 28119121)
19. Korsmeyer SJ, Wei MC, Saito M, Weiler S, Oh KJ, Schlesinger PH. Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c. Cell Death Differ 2000; 7:1166-73. (Pubitemid 32042223)
20. Adrain C, Martin SJ. The mitochondrial apoptosome: a killer unleashed by the cytochrome seas. Trends Biochem Sci 2001; 26:390-7. (Pubitemid 32530658)
21. Kataoka T, Schroter M, Hahne M, Schneider P, Irmler M, Thome M, et al. FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation. J Immunol 1998; 161:3936-42. (Pubitemid 28468969)
22. Uslu R, Borsellino N, Frost P, Garban H, Ng CP, Mizutani Y, et al. Chemosensitization of human prostate carcinoma cell lines to anti-fas- mediated cytotoxicity and apoptosis. Clin Cancer Res 1997; 3:963-72. (Pubitemid 27252945)
23. Rokhlin OW, Glover RA, Cohen MB. Fas-mediated apoptosis in human prostatic carcinoma cell lines occurs via activation of caspase-8 and caspase-7. Cancer Res 1998; 58:5870-5. (Pubitemid 29006431)
24. Schneider P, Holler N, Bodmer JL, Hahne M, Frei K, Fontana A, et al. Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity. J Exp Med 1998; 187:1205-13. (Pubitemid 28189110)
25. Bennett M, Macdonald K, Chan SW, Luzio JP, Simari R, Weissberg P. Cell surface trafficking of Fas: a rapid mechanism of p53-mediated apoptosis. Science 1998; 282:290-3. (Pubitemid 28487200)