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Volumn 282, Issue 5387, 1998, Pages 290-293

Cell surface trafficking of Fas: A rapid mechanism of p53-mediated apoptosis

Author keywords

[No Author keywords available]

Indexed keywords

FAS ANTIGEN; PROTEIN P53;

EID: 0032500793     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5387.290     Document Type: Article
Times cited : (667)

References (37)
  • 2
    • 0027359827 scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1993) Cell , vol.75 , pp. 817
    • El Deiry, W.S.1
  • 3
    • 0026496885 scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1992) Cell , vol.71 , pp. 587
    • Kastan, M.1
  • 4
    • 0028864667 scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1995) Nature , vol.377 , pp. 646
    • Buckbinder, L.1
  • 5
    • 0028335717 scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1994) Oncogene , vol.9 , pp. 1799
    • Miyashita, T.1
  • 6
    • 0030802364 scopus 로고    scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1997) EMBO J. , vol.16 , pp. 4384
    • Israeli, D.1
  • 7
    • 0030930366 scopus 로고    scopus 로고
    • W. S. el Deiry et al., Cell 75, 817 (1993); M. Kastan et al., ibid. 71, 587 (1992); L. Buckbinder et al., Nature 377, 646 (1995); T. Miyashita et al., Oncogene 9, 1799 (1994); D. Israeli et al., EMBO J. 16, 4384 (1997); K. Polyak, Y. Xia, J. L. Zweier, K. W. Kinzler, B. Vogelstein, Nature 389, 300 (1997).
    • (1997) Nature , vol.389 , pp. 300
    • Polyak, K.1    Xia, Y.2    Zweier, J.L.3    Kinzler, K.W.4    Vogelstein, B.5
  • 9
    • 0029585933 scopus 로고
    • E. Yonish-Rouach et al., Oncogene 11, 2197 (1995); L. Donatella Attardi, S. Lowe, J. Brugarolas, T. Jacks, EMBO J. 15, 3693 (1996); P. Sabbatini, J. Lin, A. Levine, E. White, Genes Dev. 9, 2184 (1995).
    • (1995) Oncogene , vol.11 , pp. 2197
    • Yonish-Rouach, E.1
  • 10
    • 0030013337 scopus 로고    scopus 로고
    • E. Yonish-Rouach et al., Oncogene 11, 2197 (1995); L. Donatella Attardi, S. Lowe, J. Brugarolas, T. Jacks, EMBO J. 15, 3693 (1996); P. Sabbatini, J. Lin, A. Levine, E. White, Genes Dev. 9, 2184 (1995).
    • (1996) EMBO J. , vol.15 , pp. 3693
    • Donatella Attardi, L.1    Lowe, S.2    Brugarolas, J.3    Jacks, T.4
  • 11
    • 0029143395 scopus 로고
    • E. Yonish-Rouach et al., Oncogene 11, 2197 (1995); L. Donatella Attardi, S. Lowe, J. Brugarolas, T. Jacks, EMBO J. 15, 3693 (1996); P. Sabbatini, J. Lin, A. Levine, E. White, Genes Dev. 9, 2184 (1995).
    • (1995) Genes Dev. , vol.9 , pp. 2184
    • Sabbatini, P.1    Lin, J.2    Levine, A.3    White, E.4
  • 12
    • 0028070989 scopus 로고
    • C. Caelles, A. Helmberg, M. Karin, Nature 370, 220 (1994); A. J. Wagner, J. M. Kokontis, N. Hay, Genes Dev. 8, 2817 (1994).
    • (1994) Nature , vol.370 , pp. 220
    • Caelles, C.1    Helmberg, A.2    Karin, M.3
  • 14
    • 0030021748 scopus 로고    scopus 로고
    • S. Rowan et al., EMBO J. 15, 827 (1996).
    • (1996) EMBO J. , vol.15 , pp. 827
    • Rowan, S.1
  • 16
    • 0029028352 scopus 로고
    • Y. Haupt, S. Rowan, E. Shaulian, K. Vousden, M. Oren, Genes Dev. 9, 2170 (1995); C. Ishioka et al., Oncogene 10, 1485 (1995).
    • (1995) Oncogene , vol.10 , pp. 1485
    • Ishioka, C.1
  • 17
    • 3643122701 scopus 로고    scopus 로고
    • note
    • ™ was transfected into PA317 retroviral packaging cells. Recombinant retrovirus harvested at 48 hours was used to infect VSMCs, rat 1 fibroblasts, or MEFs in polybrene (8 μg/ml). Cells were selected in puromycin (2.5 μg/ml) and resistant cells were pooled.
  • 21
    • 0025916387 scopus 로고
    • S. Yonehara, A. Ishii, M. Yonehara, J. Exp. Med. 169, 1747 (1989); N. Itoh et al., Cell 66, 233 (1991).
    • (1991) Cell , vol.66 , pp. 233
    • Itoh, N.1
  • 22
    • 3643116479 scopus 로고    scopus 로고
    • note
    • 6) were incubated with CH-11 (20 μg/ml) for 30 min on ice followed by a secondary fluorescein isothiocyanate-conjugated anti-mouse IgG (Sigma). Total Fas expression was analyzed by permeabilizing cells in ethanol for 30 min before Fas staining. Higher expression of cytoplasmic compared with surface Fas was evident in rat and human fibroblasts, mesangial cells, and rat VSMCs, but not in THP-1 or HeLa cells.
  • 24
    • 0026472647 scopus 로고
    • R. Klausner, J. Donaldson, J. Lippincott-Schwartz, J. Cell Biol. 116, 1071 (1992); M. Ladinsky and K. Howell, Eur. J. Cell Biol. 59, 92 (1992).
    • (1992) Eur. J. Cell Biol. , vol.59 , pp. 92
    • Ladinsky, M.1    Howell, K.2
  • 27
    • 0028223847 scopus 로고
    • R. Watanabe-Fukunaga, C. I. Brannan, N. G. Copeland, N. A. Jenkins, S. Nagata, Nature 356, 314 (1992); T. Takahashi et al., Cell 76, 969 (1994).
    • (1994) Cell , vol.76 , pp. 969
    • Takahashi, T.1
  • 29
    • 0026728952 scopus 로고
    • C. A. Ray et al., Cell 69, 597 (1992); M. Tewari and V. Dixit, J. Biol. Chem. 270, 597 (1995); K. Smith, A. Strasser, D. Vaux, EMBO J. 15, 5167 (1996).
    • (1992) Cell , vol.69 , pp. 597
    • Ray, C.A.1
  • 30
    • 0026728952 scopus 로고
    • C. A. Ray et al., Cell 69, 597 (1992); M. Tewari and V. Dixit, J. Biol. Chem. 270, 597 (1995); K. Smith, A. Strasser, D. Vaux, EMBO J. 15, 5167 (1996).
    • (1995) J. Biol. Chem. , vol.270 , pp. 597
    • Tewari, M.1    Dixit, V.2
  • 31
    • 0029834497 scopus 로고    scopus 로고
    • C. A. Ray et al., Cell 69, 597 (1992); M. Tewari and V. Dixit, J. Biol. Chem. 270, 597 (1995); K. Smith, A. Strasser, D. Vaux, EMBO J. 15, 5167 (1996).
    • (1996) EMBO J. , vol.15 , pp. 5167
    • Smith, K.1    Strasser, A.2    Vaux, D.3
  • 32
    • 3643086214 scopus 로고    scopus 로고
    • note
    • Time-lapse videomicroscopy examined the course of 100 randomly selected cells over 24 hours. Cumulative apoptotic deaths at 4-hour intervals were plotted against time. Mean deaths for each time point and experimental condition for a minimum of three independent experiments are given. Videomicroscopic measurements of apoptosis were confirmed against trypan blue exclusion (a measure of loss of viability).
  • 33
    • 3643080016 scopus 로고    scopus 로고
    • note
    • ™ VSMC lysates were incubated with anti-Fas-protein G complexes (3 hours, 4°C), precipitated, separated by 10% SDS-polyacrylamide gel electrophoresis (PAGE) in nonreducing conditions, and blotted with mouse anti-human FADD IgC (1 μg/ml).
  • 34
    • 3643118513 scopus 로고    scopus 로고
    • note
    • 2], and protease inhibitor cocktail (PIC, Sigma), resuspended in an equal volume of KEHM, and homogenized. The homogenate was centrifuged through a discontinuous sucrose gradient (1.6, 1.2, or 0.8 M sucrose in KEHM and PIC), and the Golgi-enriched fraction was collected from the 0.8/1.2 M sucrose interface, separated by 10% SDS-PAGE, blotted, and probed with mouse anti-Fas IgG (100 ng/ml) (#F22120, Transduction Labs). Fractions were compared against homogenate, and whole-cell lysate was isolated for protein immunoblots.
  • 35
    • 0030667376 scopus 로고    scopus 로고
    • Retroviruses encoding pBabe Hygro FADD-DN or control vector (pBabe Hygro) [A. O. Hueber et al., Science 278, 1305 (1997)] were synthesized as in (10), cells were selected in hygromycin (200 μg/ml), and resistant cells were pooled.
    • (1997) Science , vol.278 , pp. 1305
    • Hueber, A.O.1
  • 36
    • 0028040836 scopus 로고
    • A Nru-Dra III fragment from pcDNA3 crmA (16), cloned into the adenovirus shuttle plasmid pAdBglII, was cotransfected with Xba I-Cla I-digested sub360 genomic DNA [T. Ohno et al., Science 265, 781 (1994)] into 293 cells. Recombinant virus was isolated by plaque purification, propagated in 293 cells, and purified by caesium chloride centrifugation. Virus titers were determined by plaque assay. Target cell infection of 100% was obtained with a multiplicity of infection of 100 per cell.
    • (1994) Science , vol.265 , pp. 781
    • Ohno, T.1
  • 37
    • 3643132020 scopus 로고    scopus 로고
    • note
    • ™, E. Nabel and G. Nabel for crmA adenovirus, D. Galloway for pLHPV-E6 type 16, A. Hueber for lpr and gld MEFs and pBabe DN-FADD, T. Suganuma for anti-galactosyl transferase, and F. Buss, G. Evan, and M. Oren for advice and comments. Supported by British Heart Foundation grants FS/ 97024, PG/95057, and PG/96040 (to M.B.) and CH/ 9400 (to P. W.), Medical Research Council grant G9310915 (to J.P.L), and NIH grant HL34073 and the Bruce and Ruth Rappaport Program in Vascular Biology (to R.S.).


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