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1
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0033118214
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Managing hypoosmotic stress: Aquaporins and mechanosensitive channels in Escherichia coli
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Booth I.R., Louis P. Managing hypoosmotic stress: aquaporins and mechanosensitive channels in Escherichia coli. Curr Opin Microbiol. 2:1999;166-169.
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(1999)
Curr Opin Microbiol
, vol.2
, pp. 166-169
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Booth, I.R.1
Louis, P.2
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2
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0026525773
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Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria
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Berrier C., Coulombe A., Szabo I., Zoratti M., Ghazi A. Gadolinium ion inhibits loss of metabolites induced by osmotic shock and large stretch-activated channels in bacteria. Eur J Biochem. 206:1992;559-565.
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Eur J Biochem
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Berrier, C.1
Coulombe, A.2
Szabo, I.3
Zoratti, M.4
Ghazi, A.5
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3
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0345196593
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Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: Identification of genes required for MscS activity
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Levina N., Totemeyer S., Stokes N.R., Louis P., Jones M.A., Booth I.R. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 18:1999;1730-1737.
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(1999)
EMBO J
, vol.18
, pp. 1730-1737
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Levina, N.1
Totemeyer, S.2
Stokes, N.R.3
Louis, P.4
Jones, M.A.5
Booth, I.R.6
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4
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0035085703
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Mechanosensitive channels in prokaryotes
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Martinac B. Mechanosensitive channels in prokaryotes. Cell Physiol Biochem. 11:2001;61-76.
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(2001)
Cell Physiol Biochem
, vol.11
, pp. 61-76
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Martinac, B.1
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5
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0041929590
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Structure and mechanism in prokaryotic mechanosensitive channels
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Perozo E., Rees D.C. Structure and mechanism in prokaryotic mechanosensitive channels. Curr Opin Struct Biol. 13:2003;432-442.
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(2003)
Curr Opin Struct Biol
, vol.13
, pp. 432-442
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Perozo, E.1
Rees, D.C.2
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7
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0037335595
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Two families of mechanosensitive channel proteins
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Pivetti C.D., Yen M.R., Miller S., Busch W., Tseng Y.H., Booth I.R., Saier M.H. Two families of mechanosensitive channel proteins. Microbiol Mol Biol Rev. 67:2003;66-85.
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(2003)
Microbiol Mol Biol Rev
, vol.67
, pp. 66-85
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Pivetti, C.D.1
Yen, M.R.2
Miller, S.3
Busch, W.4
Tseng, Y.H.5
Booth, I.R.6
Saier, M.H.7
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8
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2242431668
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Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel
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This paper describes the structure of the MscS channel from E. coli. The protein is shown to exhibit a heptameric organisation in which the TM3 helices are tightly packed to create the pore. Unexpectedly, the TM1-TM2 helices are shown to contain arginine residues that are embedded in the lipid bilayer and can explain the voltage-sensitivity of the channel. The organisation of the carboxy-terminal domains to create a large vestibule suggests ways in which solutes could be pre-filtered prior to exit via the channel.
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Bass R.B., Strop P., Barclay M., Rees D.C. Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science. 298:2002;1582-1587 This paper describes the structure of the MscS channel from E. coli. The protein is shown to exhibit a heptameric organisation in which the TM3 helices are tightly packed to create the pore. Unexpectedly, the TM1-TM2 helices are shown to contain arginine residues that are embedded in the lipid bilayer and can explain the voltage-sensitivity of the channel. The organisation of the carboxy-terminal domains to create a large vestibule suggests ways in which solutes could be pre-filtered prior to exit via the channel.
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(2002)
Science
, vol.298
, pp. 1582-1587
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Bass, R.B.1
Strop, P.2
Barclay, M.3
Rees, D.C.4
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9
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0037413692
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Domain organization of the MscS mechanosensitive channel of Escherichia coli
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This paper provides independent confirmation of the organisation of the MscS channel protein. The importance of the TM3 helix for pore formation is confirmed by the identification of gain-of-function mutations that are located in this region. The paper also reports the influence of deletions on the stability of the MscS protein and its ability to function as a channel.
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Miller S., Bartlett W., Chandrasekaran S., Simpson S., Edwards M., Booth I.R. Domain organization of the MscS mechanosensitive channel of Escherichia coli. EMBO J. 22:2003;36-46 This paper provides independent confirmation of the organisation of the MscS channel protein. The importance of the TM3 helix for pore formation is confirmed by the identification of gain-of-function mutations that are located in this region. The paper also reports the influence of deletions on the stability of the MscS protein and its ability to function as a channel.
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(2003)
EMBO J
, vol.22
, pp. 36-46
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Miller, S.1
Bartlett, W.2
Chandrasekaran, S.3
Simpson, S.4
Edwards, M.5
Booth, I.R.6
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10
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0042858140
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The closed structure of the MscS mechanosensitive channel - Cross-linking of single cysteine mutants
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This work provides the first insights into the closed-to-open transition in the MscS channel. The paper shows that significant molecular rearrangements would be necessary during the gating transition. The authors also confirm the heptameric structure of the MscS channel by cross-linking experiments.
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Miller S., Edwards M.D., Ozdemir C., Booth I.R. The closed structure of the MscS mechanosensitive channel - cross-linking of single cysteine mutants. J Biol Chem. 278:2003;32246-32250 This work provides the first insights into the closed-to-open transition in the MscS channel. The paper shows that significant molecular rearrangements would be necessary during the gating transition. The authors also confirm the heptameric structure of the MscS channel by cross-linking experiments.
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(2003)
J Biol Chem
, vol.278
, pp. 32246-32250
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Miller, S.1
Edwards, M.D.2
Ozdemir, C.3
Booth, I.R.4
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11
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0037008724
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Functional design of bacterial mechanosensitive channels - Comparisons and contrasts illuminated by random mutagenesis
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This paper reports the reconstitution of the MscS channel protein into liposomes and also describes the first gain-of-function mutations, which reside in TM1 and which may affect the voltage-gating of the channel.
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Okada K., Moe P.C., Blount P. Functional design of bacterial mechanosensitive channels - comparisons and contrasts illuminated by random mutagenesis. J Biol Chem. 277:2002;27682-27688 This paper reports the reconstitution of the MscS channel protein into liposomes and also describes the first gain-of-function mutations, which reside in TM1 and which may affect the voltage-gating of the channel.
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(2002)
J Biol Chem
, vol.277
, pp. 27682-27688
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Okada, K.1
Moe, P.C.2
Blount, P.3
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12
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0036280794
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Purification of the small mechanosensitive channel of Escherichia coli (MscS): The subunit structure, conduction, and gating characteristics in liposomes
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Reconstitution of MscS is shown for the first time and confirms that a single protein alone is required for MscS activity.
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Sukharev S. Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Biophys J. 83:2002;290-298 Reconstitution of MscS is shown for the first time and confirms that a single protein alone is required for MscS activity.
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(2002)
Biophys J
, vol.83
, pp. 290-298
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Sukharev, S.1
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13
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0036169863
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Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli
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This study describes the first gain-of-function mutation in the MscK channel and reports the physiological consequences of inappropriate channel activation.
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McLaggan D., Jones M.A., Gouesbet G., Levina N., Lindey S., Epstein W., Booth I.R. Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli. Mol Microbiol. 43:2002;521-536 This study describes the first gain-of-function mutation in the MscK channel and reports the physiological consequences of inappropriate channel activation.
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(2002)
Mol Microbiol
, vol.43
, pp. 521-536
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McLaggan, D.1
Jones, M.A.2
Gouesbet, G.3
Levina, N.4
Lindey, S.5
Epstein, W.6
Booth, I.R.7
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14
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12444274301
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Crystal structure of the potassium channel KirBac1.1 in the closed state
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Kuo A.L., Gulbis J.M., Antcliff J.F., Rahman T., Lowe E.D., Zimmer J., Cuthbertson J., Ashcroft F.M., Ezaki T., Doyle D.A. Crystal structure of the potassium channel KirBac1.1 in the closed state. Science. 300:2003;1922-1926.
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(2003)
Science
, vol.300
, pp. 1922-1926
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Kuo, A.L.1
Gulbis, J.M.2
Antcliff, J.F.3
Rahman, T.4
Lowe, E.D.5
Zimmer, J.6
Cuthbertson, J.7
Ashcroft, F.M.8
Ezaki, T.9
Doyle, D.A.10
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15
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0037107398
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Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli
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+ and the voltage-independence of the channel are described. The study also presents electrophysiological characterisation of a gain-of-function mutation in MscK.
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+ and the voltage-independence of the channel are described. The study also presents electrophysiological characterisation of a gain-of-function mutation in MscK.
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(2002)
EMBO J
, vol.21
, pp. 5323-5330
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Li, Y.1
Moe, P.C.2
Chandrasekaran, S.3
Booth, I.R.4
Blount, P.5
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16
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0035901511
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Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii
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Kloda A., Martinac B. Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii. EMBO J. 20:2001;1888-1896.
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(2001)
EMBO J
, vol.20
, pp. 1888-1896
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Kloda, A.1
Martinac, B.2
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17
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0000574498
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Pressure-sensitive ion channel in Escherichia coli
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Martinac B., Buehner M., Delcour A.H., Adler J., Kung C. Pressure-sensitive ion channel in Escherichia coli. Proc Natl Acad Sci U S A. 84:1987;2297-2301.
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Martinac, B.1
Buehner, M.2
Delcour, A.H.3
Adler, J.4
Kung, C.5
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18
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0032545321
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Structure of the MscL homolog from Mycobacterium tuberculosis: A gated mechanosensitive ion channel
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Chang G., Spencer R.H., Lee A.T., Barclay M.T., Rees D.C. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science. 282:1998;2220-2226.
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Science
, vol.282
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Chang, G.1
Spencer, R.H.2
Lee, A.T.3
Barclay, M.T.4
Rees, D.C.5
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19
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0029909197
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Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli
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Blount P., Sukharev S.I., Schroeder M.J., Nagle S.K., Kung C. Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli. Proc Natl Acad Sci U S A. 93:1996;11652-11657.
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(1996)
Proc Natl Acad Sci U S a
, vol.93
, pp. 11652-11657
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Blount, P.1
Sukharev, S.I.2
Schroeder, M.J.3
Nagle, S.K.4
Kung, C.5
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20
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0032530833
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One face of a transmembrane helix is crucial in mechanosensitive channel gating
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Ou X., Blount P., Hoffman R.J., Kung C. One face of a transmembrane helix is crucial in mechanosensitive channel gating. Proc Natl Acad Sci U S A. 95:1998;11471-11475.
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(1998)
Proc Natl Acad Sci U S a
, vol.95
, pp. 11471-11475
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Ou, X.1
Blount, P.2
Hoffman, R.J.3
Kung, C.4
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21
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7144257172
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Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation
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Moe P.C., Blount P., Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 28:1998;583-592.
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(1998)
Mol Microbiol
, vol.28
, pp. 583-592
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Moe, P.C.1
Blount, P.2
Kung, C.3
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22
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0038080063
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Generation and evaluation of a large mutational library from the Escherichia coli mechanosensitive channel of large conductance, MscL - Implications for channel gating and evolutionary design
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Maurer J.A., Dougherty D.A. Generation and evaluation of a large mutational library from the Escherichia coli mechanosensitive channel of large conductance, MscL - implications for channel gating and evolutionary design. J Biol Chem. 278:2003;21076-21082.
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(2003)
J Biol Chem
, vol.278
, pp. 21076-21082
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Maurer, J.A.1
Dougherty, D.A.2
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23
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0037334905
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On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL
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Anishkin A., Gendel V., Sharifi N.A., Chiang C.S., Shirinian L., Guy H.R., Sukharev S. On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL. J Gen Physiol. 121:2003;227-244.
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(2003)
J Gen Physiol
, vol.121
, pp. 227-244
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Anishkin, A.1
Gendel, V.2
Sharifi, N.A.3
Chiang, C.S.4
Shirinian, L.5
Guy, H.R.6
Sukharev, S.7
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24
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0034910316
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Structural models of the MscL gating mechanism
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Sukharev S., Durell S.R., Guy H.R. Structural models of the MscL gating mechanism. Biophys J. 81:2001;917-936.
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, vol.81
, pp. 917-936
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Sukharev, S.1
Durell, S.R.2
Guy, H.R.3
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25
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0035825634
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The gating mechanism of the large mechanosensitive channel MscL
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Sukharev S., Betanzos M., Chiang C.S., Guy H.R. The gating mechanism of the large mechanosensitive channel MscL. Nature. 409:2001;720-724.
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(2001)
Nature
, vol.409
, pp. 720-724
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Sukharev, S.1
Betanzos, M.2
Chiang, C.S.3
Guy, H.R.4
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26
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0034884665
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Site-directed spin-labelling analysis of reconstituted MscL in the closed state
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Perozo E., Kloda A., Cortes D.M., Martinac B. Site-directed spin-labelling analysis of reconstituted MscL in the closed state. J Gen Physiol. 118:2001;193-206.
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(2001)
J Gen Physiol
, vol.118
, pp. 193-206
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Perozo, E.1
Kloda, A.2
Cortes, D.M.3
Martinac, B.4
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27
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0037194760
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Open channel structure of MscL and the gating mechanism of mechanosensitive channels
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Analysis of the open structure of MscL is achieved by site-directed cysteine-specific spin-labelling combined with altering the physical state of the membrane. This allowed the protein to be captured in a range of intermediate states between closed and fully open.
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Perozo E., Cortes D.M., Sompornpisut P., Kloda A., Martinac B. Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Nature. 418:2002;942-948 Analysis of the open structure of MscL is achieved by site-directed cysteine-specific spin-labelling combined with altering the physical state of the membrane. This allowed the protein to be captured in a range of intermediate states between closed and fully open.
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(2002)
Nature
, vol.418
, pp. 942-948
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Perozo, E.1
Cortes, D.M.2
Sompornpisut, P.3
Kloda, A.4
Martinac, B.5
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28
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0036725152
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Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating
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Analysis of the gating transition of MscL is achieved by site-directed cysteine-specific spin-labelling using amphiphiles to activate the MscL channel in liposomes.
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Perozo E., Kloda A., Cortes D.M., Martinac B. Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating. Nat Struct Biol. 9:2002;696-703 Analysis of the gating transition of MscL is achieved by site-directed cysteine-specific spin-labelling using amphiphiles to activate the MscL channel in liposomes.
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(2002)
Nat Struct Biol
, vol.9
, pp. 696-703
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Perozo, E.1
Kloda, A.2
Cortes, D.M.3
Martinac, B.4
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29
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0037500247
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C-termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening
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Koprowski P., Kubalski A. C-termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening. J Biol Chem. 278:2003;11237-11245.
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(2003)
J Biol Chem
, vol.278
, pp. 11237-11245
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Koprowski, P.1
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30
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0036470051
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Protein explorer: Easy yet powerful macromolecular visualization
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Martz E. Protein explorer: easy yet powerful macromolecular visualization. Trends Biochem Sci. 27:2002;107-109.
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Trends Biochem Sci
, vol.27
, pp. 107-109
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Martz, E.1
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0347994890
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A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS
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Stokes N.R., Murray H.D., Subramaniam C., Gourse R.L., Louis P., Bartlett W., Miller S., Booth I.R. A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS. Proceedings of the National Academy of Sciences of the United States of America. 100:2003;15959-15964.
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(2003)
Proceedings of the National Academy of Sciences of the United States of America
, vol.100
, pp. 15959-15964
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Stokes, N.R.1
Murray, H.D.2
Subramaniam, C.3
Gourse, R.L.4
Louis, P.5
Bartlett, W.6
Miller, S.7
Booth, I.R.8
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