Purification of the small mechanosensitive channel of Escherichia coli (MscS): The subunit structure, conduction, and gating characteristics in liposomes

Biophysical Journal

Volumn 83, Issue 1, 2002, Pages 290-298

  Sukharev, Sergei ^a  

^a Bldg 142   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOSOME; PROTEIN SUBUNIT;

ARTICLE; CELL PROTECTION; CHANNEL GATING; CROSS LINKING; ESCHERICHIA COLI; MEMBRANE CURRENT; NONHUMAN; OSMOLARITY; PATCH CLAMP; PRESSURE GRADIENT; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; SOLUBILIZATION; SPHEROPLAST;

ESCHERICHIA COLI; GLYCINE MAX;

EID: 0036280794     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75169-2     Document Type: Article

References (27)

1. Multiple mechanosensitive ion channels from Escherichia coli, activated at different thresholds of applied pressure
2. Mechanosensitive channels of bacteria
3. Membrane topology and multimeric structure of a mechanosensitive channel protein of Escherichia coli
4. Managing hypoosmotic stress: Aquaporins and mechanosensitive channels in Escherichia coli
5. Structure of the MscL homolog from Mycobacterium tuberculosis: A gated mechanosensitive ion channel
6. Gravitropism in higher plants
7. Prokaryotic osmoregulation: Genetics and physiology
8. Effect of mutation of potassium-efflux system, KefA, on mechanosensitive channels in the cytoplasmic membrane of Escherichia coli
9. Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels
10. Molecular basis of mechanosensory transduction
11. The cloning of a mechanogated membrane ion channel
12. Molecular identification of a mechanosensitive channel in archaea
13. Microbial physiology and ecology of slow growth
14. Voltage-independent adaptation of mechanosensitive channels in Escherichia coli protoplasts
15. Bacterial ion channels and their eukaryotic homologues
16. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: Identification of genes required for MscS activity
17. Pressure-sensitive ion channel in Escherichia coli
18. Analysis of the kefA mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli
19. Meningococcal PorA/C1, a channel that combines high conductance and high selectivity
20. "Feeling the pressure": Structural insights into a gated mechanosensitive channel
21. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone
22. Two types of mechanosensitive channels in the Escherichia coli cell envelope: Solubilization and functional reconstitution
23. Stoichiometry of the large conductance bacterial mechanosensitive channel of E. coli: A biochemical study
24. Energetic and spatial parameters for gating of the bacterial large conductance mechanosensitive channel, MscL
25. Osmosensing by bacteria: Signals and membrane-based sensors
26. Zero-current potentials in a large membrane channel: A simple theory accounts for complex behavior