Immortalization of human corneal endothelial cells using electroporation protocol optimized for human corneal endothelial and human retinal pigment epithelial cells

Acta Ophthalmologica Scandinavica

Volumn 78, Issue 2, 2000, Pages 130-136

  Bednarz, Jürgen ^a   Teifel, Michael ^b   Friedl, Peter ^b   Engelmann, Katrin ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Electroporation; Human corneal endothelial cells (HCEC); Human retinal pigment epithelial cells (HRPE); Immortalization.; Transfection

Indexed keywords

VIRUS SMALL T ANTIGEN;

ARTICLE; CELL CULTURE; CELL IMMORTALIZATION; CELL STRUCTURE; CELL SURVIVAL; CELL TYPE; CLINICAL PROTOCOL; CONTROLLED STUDY; CORNEA ENDOTHELIUM; ELECTROPORATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PIGMENT EPITHELIUM; PLASMID; PRIORITY JOURNAL;

ADULT; AGED; AGED, 80 AND OVER; ANTIGENS, POLYOMAVIRUS TRANSFORMING; BETA-GALACTOSIDASE; BLOTTING, WESTERN; CELL DIVISION; CELL LINE, TRANSFORMED; CELL SURVIVAL; CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPORATION; ENDOTHELIUM, CORNEAL; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, PHASE-CONTRAST; MIDDLE AGED; PIGMENT EPITHELIUM OF EYE; PLASMIDS; TRANSFECTION;

EID: 0034127821     PISSN: 13953907     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0420.2000.078002130.x     Document Type: Article

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