Morphological and functional analysis of immortalized human corneal endothelial cells after transplantation

Experimental Eye Research

Volumn 69, Issue 5, 1999, Pages 547-553

  Aboalchamat, Bilal ^a   Engelmann, Katrin ^a   Böhnke, Matthias ^b   Eggli, Peter ^b   Bednarz, Jürgen ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b University Eye Clinic Bern   (Switzerland)

Author keywords

Cell transplantation; Cornea; Cornea perfusion; Human corneal endothelial cells (HCEC); Human corneal endothelium; Pump function; SV40

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); OUABAIN;

ARTICLE; CELL IMMORTALIZATION; CELL TRANSPLANTATION; CORNEA CELL; CORNEA ENDOTHELIUM; CORNEA TRANSPLANTATION; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SIMIAN VIRUS 40;

EID: 0032701412     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.1999.0736     Document Type: Article

References (40)

1. Alvarado J. A., Gospodarovicz D., Greenberg G. Corneal endothelial replacement. In vitro formation of an endothelial monolayer. Invest. Ophthalmol. Vis. Sci. 21:1981;300-316.
2. Bednarz, J., Engelmann, K., Rodokanaki-von Schrenck, A., Friedl, P., Teifel, M. and Richard, G. (1996). Transfektion humaner kornealer Endothelzellen sowie humaner retinaler Pigmentepithelzellen. Ophthalmologe 93, S103.
3. Bednarz, J., Friedl, P. and Engelmann, K. (1998). Charakterisierung humaner kornealer Endothelzellen nach Transfektion mit dem gro†en und kleinen T-Antigen des SV40. Ophthalmologe 95, S131.
4. Böhnke, M. (1983). Möglichkeiten zur Beurteilung des Endothels frischer und konservierter Hornhäute. Klin. Mbl. Augenhlk. 182, 595-6.
5. Böhnke M. Corneal preservation in organ culture. Curr. Opinion Ophthalmol. 2:1991;432-442.
6. Böhnke M., Thaer A. A. Untersuchung der kornea mit einem neuen konfokalen mikroskop. Lund O. E., Waubke T. N. Bildgebende Verfahren in der Augenheilkunde. 1994;47-53 Enke.
7. Böhnke M., Masters B. R. Long term contact lens wear induces a corneal degeneration with micro-dot deposits in the corneal stroma. Ophthalmology. 104:1997;1887-1896.
8. Böhnke M., Eggli P., Engelmann K. Morphology of endothelial cell transplantation onto human corneas. Part II: Evaluation with scanning electron microscopy. Cornea. 18:1999;207-213.
9. Bourne W. M. Chronic endothelial cell loss in transplanted corneas. Cornea. 2:1983;289-294.
10. Bourne W. M., Kaufman H. E. Specular microscopy of human corneal endothelium in vivo. Am. J. Ophthalmol. 81:1976;319-326.
11. Bourne W. M., O'Fallon W. M. Endothelial cell loss during penetrating keratoplasty. Am. J. Ophthalmol. 85:1978;760-766.
12. Capella J. A. Regeneration of endothelium in diseased and injured corneas. Am. J. Ophthalmol. 74:1972;810-817.
13. Dikstein S., Maurice D. M. The metabolic basis of the fluid pumps in the cornea. J. Physiol. 221:1972;29-41.
14. Engelmann K., Böhnke M., Friedl P. Isolation and long-term cultivation of human corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 29:1988;1656-1662.
15. Engelmann K., Friedl P. Optimization of culture conditions for human corneal endothelial cells. In Vitro. Cell. Dev. Bio. 25:1989;1065-1072.
16. Engelmann K., Böhnke M. Human corneal endothelial cells in long-term cultures: The influence of conditions for isolation, selective and normal growth, and the extra-cellular matrix on proliferation and morphology. Chibret Int. J. Ophthalmol. 7:1990;3-13.
17. Engelmann K., Friedl P. Growth of human corneal endothelial cells in a serum-reduced medium. Cornea. 14:1995;62-70.
18. Engelmann K., Drexler D., Draeger J., Böhnke M. Endothelzelltransplantation im Modell. Ophthalmologe. 90:1993;166-170.
19. Engelmann K., Drexler D., Böhnke M. Transplantation of adult human or porcine corneal endothelial cells onto human corneas in vitro. Part I: Cell culturing and transplantation procedure. Cornea. 18:1999;199-206.
20. Fischbarg J., Lim J. J. Role of cations, anions and carbonic anhydrase in fluid transport across rabbit corneal endothelium. J. Physiol. 241:1974;647-675.
21. Geroski H. H., Matsuda M., Yee R. W., Edelhauser H. F. Pump function of the human corneal endothelium. Effects of age and cornea guttata. Ophthalmology. 92:1985;759-763.
22. Gospodarowicz D., Greenburg G., Alvarado J. Transplantation of cultured bovine corneal endothelial cells to rabbit cornea: Clinical implication for human studies. Proc. Natl. Acad. Sci. USA. 76:1979;464-468.
23. Hodson S. Evidence for a bicarbonate-dependent sodium pump in corneal endothelium. Exp. Eye Res. 11:1971;20-29.
24. Hodson S., Wigham C., Williams L., Mayes K. R., Graham M. V. Observations on the Human Cornea in vitro. Exp. Eye Res. 32:1981;353-360.
25. Hoppenreijs V. P. T., Pels E., Vresen G. F. J. M., Treffers W. F. Basic fibroblast growth factor stimulates corneal endothelial cell growth and endothelial wound healing of human corneas. Invest. Ophthalmol. Vis. Sci. 35:1994;931-944.
26. Insler M. S., Lopez J. G. Extended incubation times improve corneal endothelial cell transplantation success. Invest. Ophthalmol. Vis. Sci. 32:1991;1828-1836.
27. Jumblatt M. M., Maurice D. M., McCulley J. P. Transplantation of tissue-cultured corneal endothelium. Invest. Ophthalmol. Vis. Sci. 17:1978;11135-11141.
28. Kaufman H. E., Capella J. A., Robbins J. E. The human corneal endothelium. Am. J. Ophthalmol. 61:1966;835-841.
29. Kloss A., Böhnke M. In-vitro-Untersuchung der Endothelverträglichkeit von 10 Kulturmedien unter besonderer Berücksichtigung der Pump- und Barrierefunction. Fortschr. Ophthalmol. 88:1991;781-785.
30. Litzkas P., Jha K. K., Ozer H. L. Efficient transfer of cloned DNA into human diploid cells: Protoplast fusion in suspension. Mol. Cell. Biol. 4:1984;2549-2552.
31. Lütjen-Drecoll E., Lönnerholm G. Carbonic anhydrase distribution in the rabbit eye by light and electron microscopy. Invest. Ophthalmol. Vis. Sci. 21:1981;782-791.
32. Masters B. R., Thaer A. A. Real-time scanning slit confocal microscopy of the in vivo human cornea. Appl. Optics. 33:1994;695-701.
33. Maurice D. M. The location of the fluid pump in the cornea. J. Physiol. 221:1972;43-54.
34. Maurice D. M., McCulley J. P., Perlman M. M. Development in use of cultured endothelium in corneal transplantation. Doc. Ophthalmol. Proc. Ser. 20:1979;151-153.
35. McCulley J. P., Maurice D. M., Schwartz B. D. Corneal endothelial transplantation. Ophthalmology. 87:1980;194-201.
36. Reim M., Turss R. Metabolitspiegel im Corneaendothel und Kammerwasser bei Rinder. Graefes Arch. Clin. Exp. Ophthalmol. 215:1968;341-348.
37. Schultz G., Cipolla L., Whitehouse A., Eiferman R., Woost P., Jumblatt M. Growth factors and corneal endothelial cells: III. Stimulation of adult human corneal endothelial cell mitosis in vitro by defined mitogenic agents. Cornea. 11:1992;20-27.
38. Singh G., Böhnke M., von Domarus D., Draeger J., Lindstrom R. L., Doughman D. J. Vital staining of corneal endothelium. Cornea. 4:1985;80-91.
39. Waring G. O., Bourne W. M., Edelhauser H. F., Kenyon K. R. The corneal endothelium: Normal and pathologic structure and function. Ophthalmology. 89:1982;531-590.
40. Wiegand W., Thaer A. A., Kroll P., Geyer O. C., Garcia A. J. Optical sectioning of the cornea with a new confocal in vivo slit-scanning videomicroscope. Ophthalmology. 102:1995;568-575.