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Volumn 286, Issue 5447, 1999, Pages 2169-2172

Functional human corneal equivalents constructed from cell lines

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL IMMORTALIZATION; CELL LINE; CELL STRUCTURE; CORNEA; CORNEA CELL; ELECTROPHYSIOLOGY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE GRAFT;

EID: 0032797273     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5447.2169     Document Type: Article
Times cited : (462)

References (46)
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    • Cell lines producing successful constructs were from young donors, were immortalized with HPV E6/E7, and had appropriate electrophysiological responses.
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    • Constructs, with epithelium sealed with silicone oil to prevent movement of water across the anterior surface, were measured using OCT (Zeiss-Humphrey, San Leandro, CA). The average change in thickness was calculated from four to six measurements before and after treatment of the endothelial surface for 2 hours with 100 μM ouabain or Dulbecco's modified Eagle's medium alone (DMEM, Gibco).
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    • note
    • Supported by grants from the Natural Sciences and Engineering Research Council of Canada, Medical Research Council-Pharmaceutical Manufacturers' Association of Canada, and the P&G International Program for Animal Alternatives. We thank S. Whelan and P. Berg for AdS and psvBneo plasmids, C. Smith and Ottawa Hospital Research Institute colleagues, and E. Hay and A. Tashjian Jr. for helpful insights during preparation of this manuscript.


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