Modulation of intracellular calcium signaling and mitochondrial function in cultured osteoblastic cells by dexamethasone and celecoxib during mechanical stimulation

Clinical and Experimental Rheumatology

Volumn 22, Issue 2, 2004, Pages 184-196

  Schuchmann, Sebastian ^a,b   Wiontzek, Martin ^a   Burmester, Gerd Rüdiger ^a   Buttgereit, Frank ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Celecoxib; Gap junction; Glucocorticoid; Intracellular Ca2+ concentration; Mechanical stimulation; Mitochondrial membrane potential; Osteoblast

Indexed keywords

CALCIUM ION; CELECOXIB; CYCLOOXYGENASE 2 INHIBITOR; DEXAMETHASONE; FURA 2 ACETOXYMETHYL ESTER; GLUCOCORTICOID; RHODAMINE 123;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM SIGNALING; CELL COMMUNICATION; CELL CULTURE; CELL LINE; CELL MEMBRANE DEPOLARIZATION; CELL MEMBRANE POTENTIAL; CELL METABOLISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ACTIVITY; DRUG SELECTIVITY; ENZYME INHIBITION; GAP JUNCTION; IN VITRO STUDY; MECHANICAL STIMULATION; MICROFLUOROMETRY; MITOCHONDRIAL MEMBRANE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT;

ANIMALS; CALCIUM SIGNALING; CELL LINE, TUMOR; CYCLOOXYGENASE INHIBITORS; DEXAMETHASONE; GAP JUNCTIONS; GLUCOCORTICOIDS; INTRACELLULAR MEMBRANES; MEMBRANE POTENTIALS; MITOCHONDRIA; MODELS, BIOLOGICAL; OSTEOBLASTS; OSTEOSARCOMA; PHYSICAL STIMULATION; PYRAZOLES; RATS; STRESS, MECHANICAL; SULFONAMIDES;

EID: 1842502702     PISSN: 0392856X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (75)

1. BOWMAN NN, DONAHUE HJ, EHRLICH HP: Gap junctional intercellular communication contributes to the concentration of rat osteoblast populated collagen lattices. J Bone MinerRes 1998; 13: 1700-6.
2. DONAHUE HJ: Gap junctional intracellular communication in bone: A cellular basis for the mechanostat set point. Calcif Tissue Int 1998; 62: 85-8.
3. TANAKA Y, MORIMOTO I, NAKANO Y et al.: Osteoblasts are regulated by the cellular adhesion through ICAM-1 and VCAM-1. J Bone Miner Res 1995; 10: 1462-9.
4. Vander MOLEN MA, RUBIN CT, McLEOD KJ, McCAULEY LK, DONAHUE HJ: Gap junctional intracellular communication contributes to hormonal responsiveness in osteoblastic networks. J Biol Chem 1996; 271: 12165-71.
5. LECANDA F, TOWLER DA, ZIAMBARAS K et al.: Gap junctional communication modulates gene expression in osteoblastic cells. Mol Biol Cell 1998; 9: 2249-58.
6. SCHIRRMACHER K, SCHMITZ I, WINTERHAGER E et al.: Characterization of gap junctions between osteoblast-like cells in culture. Calcif Tissue Int 1992; 51: 285-90.
7. STEINBERG TH, CIVITELLI R, GEIST ST et al.: Connexin43 and connexin45 form gap junctions with different molecular permeabilities in osteoblastic cells. EMBO J 1994; 13: 744-50.
8. GRAMSCH B, GABRIEL H-D, WIEMANN M et al.: Enhancement of connexin 43 expression increases proliferation and differentiation of an osteoblast-like cell line. Exp Cell Res 2001; 264: 397-407.
9. CANALIS E, DELANY AM: Mechanisms of glucocorticoid action in bone. Ann N Y Acad Sci 2002; 966: 73-81.
10. SHALHOUB V, CONLON D, TASSINARI M et al.: Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genes. J Cell Biochem 1992; 50: 425-40.
11. WONG M-M, RAO LG, LY H et al.: Long-term effects of physiologic concentrations of dexamethasone on human bone-derived cells. J Bone Miner Res 1990; 5: 803-13.
12. YAMANOUCHI K, GOTCH Y, NAGAYAMA M: Dexamethasone enhances differentiation of human osteoblastic cells in vitro. J Bone Miner Res 1997; 15: 23-9.
13. KWIATKOWSKI AP, BAKER TK, KLAUNIG JE: Comparison of glucocorticoid-mediated change in expression and function of rat hepatocyte gap junctional proteins. Carcinogenesis 1994; 15: 1753-7.
14. FLADMARK KE, GJERTSEN BT, MOLVEN A, MELLGREN G, VINTERMYR OK, DOSKELAND SO: Gap junctions and growth control in liver regeneration and in isolated rat hepatocytes. Hepatology 1997; 25: 847-55.
15. PIECHOCKI MP, TOTI RM, FERNSTROM MJ, BURK RD, RUCH RJ: Liver cell-specific transcriptional regulation of connexin32. Biochim Biophys Acta 2000; 149: 107-22.
16. SIMARD M, COULDWELL WT, ZHANG W: Glucocorticoids - potent modulators of astrocytic calcium signaling. Glia 1999; 28: 1-12.
17. LIN JHC, YANG J, LIU S et al.: Connexin mediates gap junction-independent resistance to cellular injury. J Neurosci 2003; 23: 430-41.
18. CIVITELLI R, BEYER EC, WARLOW PM, ROBERTSON AJ, GEIST ST, STEINBERG TH: Connexin43 mediated direct intracellular communication in human osteoblastic cell networks. J Clin Invest 1993; 91: 1888-96.
19. ROSEN GD, BIRKENMEIER TM, RAZ A, HOLTZMAN MJ: Identification of a cyclooxygenase-related gene and its potential role in prostaglandin formation. Biochem Biophys Res Commun 1989; 164: 17-23.
20. LANGENBACH R, LOFTIN CD, LEE C, TIANO H: Cyclooxigenase-deficient mice. A summary of their characteristics and susceptibilities to inflammation and carcinogenesis. Ann NY Acad Sci 1999; 889: 52-61.
21. MIN YK, RAO Y, OKADA Y, RAISZ LG, PILBEAM CC: Regulation of prostaglandin G/H synthase-2 expression by interleukin-1 in human osteoblast-like cells. J Bone Miner Res 1998; 13: 1066-75.
22. KAWAGUCHI H, PILBEAM CC, HARRISON JR, RAISZ LG: The role of prostaglandins in the regulation of bone metabolism. Clin Orthop 1995; 313: 36-46.
23. ZHANG X, MORHAM SG, LANGENBACH R et al.: Evidence for a direct role of cyclo-oxygenase 2 in implant wear debris-induced osteolysis. J Bone Miner Res 2001; 16: 660-70.
24. FORWOOD MR: Inducible cyclo-oxygenase (COX-2) mediates the induction of bone formation by mechanical loading in vivo. J Bone Miner Res 1996; 11: 1688-93.
25. ZHANG X, SCHWARZ EM, YOUNG DA, PUZAS EJ, ROSIER RN, O'KEEFE RJ: Cyclooxygenase-2 regulates mesenchymal cell differentiation into osteoblast lineage and is critically involved in bone repair. J Clin Invest 2002; 109: 1405-15.
26. SIMON AM, MANIGRASSO MB, O'CONNOR JP: Cyclo-oxygenase 2 function is essential for bone fracture healing. J Bone Miner Res 2002; 17: 963-7.
27. ROMANELLO M, PANI, B, BICEGO M, D'ANDREA P: Mechanically induced ATP release from human osteoblastic cells Biochem Biophys Res Commun 2001; 289: 1275-81.
28. CHENG B, ZHAO S, LUO J, SPRAGUE E, BONEWALD LF, JIANG XJ: Expression of functional gap junctions and regulation by fluid flow in osteocyte-like MLO-Y4 cells. J Bone Miner Res 2001; 16: 249-59.
29. PAVALKO FM, CHEN NX, TURNER CH et al.: Fluid shearinduced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions. Am J Physiol 1998; 275: C1591-C1601.
30. PAVALKO FM, GERARD RL, PONIK SM, GALLAGHER PJ, JIN Y, NORVELL SM: Fluid shear stress inhibits TNF-induced apoptosis in osteoblasts: A role for fluid shear stress-induced activation of PI3-kinase and inhibition of caspase-3. J Cell Physiol 2003; 194: 194-205.
31. MEYER U, TERODDE M, JOOS U, WIESMANN HP: Mechanische Stimulation von Osteoblasten in der Zellkultur. Mund Kiefer Gesichts Chir 2001; 5: 166-72.
32. WEYTS FAA, BOSMANS B, NIESING R, van LEEUWEN JPTM, WEINANS H: Mechanical control of human osteoblast apoptosis and proliferation in relation to differentiation. Calcif Tissue Int 2003; 72: 505-12.
33. FITZGERALD J, HUGHES-FULFORD M: Mechanically induced c-fos expression is mediated by cAMP in MC3T3-E1 osteoblasts. FASEB J 1999; 13: 553-7.
34. ROMANELLO M, D'ANDREA P: Dual mechanism of intercellular communication in HOBIT osteoblastic cells: A role for gap-junctional hemichannels. J Bone Miner Res 2001; 16: 1465-76.
35. JØRGENSEN NR, GEIST ST, CIVITELLI R, STEINBERG TH: ATP- and Gap junction-dependent intracellular calcium signaling in osteoblastic cells. J Cell Biol 1997; 139: 497-506.
36. JØRGENSEN NR, HENRIKSEN Z, BROT C et al.: Human osteoblastic cells propagate intercellular calcium signals by two different mechanisms. J Bone Miner Res 2000; 15: 1024-32.
37. MARTIN TJ, INGLETON PM, UNDERWOOD JC, MICHELANGELI VP, HUNT NH, MELICK RA: Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 1976; 260: 436-8.
38. PARTRIDGE NC, ALCORN D, MICHELANGELI VP, RYAN G, MARTIN TJ: Morphological and biochemical characterization of four clonal osteogenic sarcoma cells of rat origin. Cancer Res 1983; 43: 4308-12.
39. PARTRIDGE NC, OPIE AL, OPIE RT, MARTIN TJ: Inhibitory effects of parathyroid hormone on the proliferation of osteoblast-like cells. Calcif Tissue Int 1985; 37: 519-25.
40. FORREST SM, NG KW, FINDLAY DM et al.: Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif Tissue Int 1985; 37: 51-56.
41. JOHNSON LV, WALSH ML, CHEN LB: Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci USA 1980; 77: 990-4.
42. EMAUS RK, GRUNWALD R, LEMASTERS JJ: Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: Spectral and metabolic properties. Biochim Biophys Acta 1986; 850: 436-48.
43. 2+ indicators with greatly improved fluorescence properties. J Biol Chem 1985; 260: 3440-50.
44. DUCHEN MR: Fluorescence - monitoring cell chemistry in vivo. In STAMFORD AJ (Ed.): Monitoring Neuronal Activity. A Practical Approach. 1992: 231-60.
45. 2+ and metabolism-related changes of mitochondrial potential in voltage-clamped CA1 pyramidal neurons in situ. J Neurophysiol 2000; 83: 1710-23.
46. HOFBAUER LC, DUNSTAN CR, SPELSBERG TC, RIGGS BL, KHOSLA S: Osteoprotegerin production by human osteoblast lineage cells is stimulated by vitamin D, bone morphogenetic protein-2, and cytokines. Biochem Biophys Res Comm 1998; 250: 776-81.
47. HUGHES FJ, BUTTERY LD, HUKKANEN MV, O'DONNELL A, MACLOUF J, POLAK JM: Cytokine-induced prostaglandin E2 synthesis and cyclooxygenase-2 activity are regulated both by a nitric oxide-dependent and -independent mechanism in rat osteoblasts in vitro. J Biol Chem 1999; 274: 1776-82.
48. ROSENQUIST JB, OHLIN A, LERNER UH: Cytokine-induced inhibition of bone matrix proteins is not mediated by prostaglandins. Inflamm Res 1996; 45: 457-63.
49. RUTZ HB: Effects of corticosteroid use on treatment of solid tumors. Lancet 2002; 360: 1669-70.
50. CHEUNG R, MITCHELL J: Mechanisms of regulation of G(11)alpha protein by dexamethasone in osteoblastic UMR 106-01 cells. Am J Physiol Endocrinol Metab 2002; 282: E24-30.
51. FELTUS FA, COTE S, SIMARD J et al.: Glucocorticoids enhance activation of the human type II 3beta-hydroxysteroid dehydrogenase/Delta5-Delta4 isomerase gene. J Steroid Biochem Mol Biol 2002; 82: 55-63.
52. GRÖSCH S, TEGEDER I, NIEDERBERGER E, BRÄUTIGAM L, GEISSLINGER G: COX-2 independent induction of cell cycle arrest and apoptosis in colon cancer cells by the selective COX-2 inhibitor celecoxib. FASEB J 2001; 15: 2742-4.
53. KUSUNOKI N, YAMAZAKI R, KAWAI S: Induction of apoptosis in rheumatoid synovial fibroblasts by celecoxib, but not by other selective cyclooxygenase 2 inhibitors. Arthritis Rheum 2002; 46, 3159-67.
54. DAVIDSON JS, BAUMGARTEN IM: Glycyrrhetinic acid derivatives: A novel class of inhibitors of gap-junctional intercellular communication. Structure-activity relationships. J Pharmacol Exp Ther 1988; 246, 1104-7.
55. SPRAY DC, HARRIS AL, BENNETT MVL: Gap junctional conductance is a simple and sensitive function of intracellular pH. Science 1981; 211: 712-5.
56. MacVICAR B, JAHNSEN H: Uncoupling of CA3 pyramidal neurons by propionate. Brain Res 1985; 330: 141-5.
57. DONAHUE HJ: Gap junctions and biophysical regulation of bone cell differentiation. Bone 2000; 26: 417-22.
58. RAWLINSON SCF, PITSILLIDES AA, LANYON LE: Involvement of different ion channels in osteoblasts' and osteocytes' early responses to mechanical strain. Bone 1996; 19: 609-14.
59. CARVALHO RS, SCOTT JE, SUGA DM, YEN EHK: Stimulation of signal transduction pathways in osteoblasts by mechanical strain potentiated by parathyroid hormone. J Bone Miner Res 1994; 9: 999-1011.
60. REHER P, HARRIS M, WHITEMAN M, HAI HK, MEGHJI S: Ultrasound stimulates nitric oxide and prostaglandin production by human osteoblasts. Bone 2002; 31: 236-41.
61. JESSOP HL, RAWLINSON SCF, PITSILLIDES AA, LANYON LE: Mechanical strain and fluid movement both activate extracellular regulated kinase (ERK) in osteoblast-like cells but via different signaling pathways. Bone 2002; 31: 186-94.
62. McLEOD KJ, DONAHUE HJ, LEVIN PE, FONTAINE MA; RUBIN CT: Electric fields modulate bone cell function in a density-dependent manner. J Bone Miner Res 1993; 8: 977-84.
63. WALSH S, JORDAN GR, JEFFERISS C, STEWART K, BERESFORD, IN: High concentrations of dexamethasone suppress the proliferation but not the differentiation or further maturation of human osteoblast precursors in vitro: Relevance to glucocorticoid-induced osteoporosis. Rheumatology (Oxford) 2001; 40: 74-83
64. KASAI Y, OHTA T, NAKAZATO Y, ITO S: Release of dopamine and ATP from PC12 cells treated with dexamethasone, reserpine and bafilomycin A1. J Vet Med Sci 2001; 63: 367-72.
65. 2 is essential for gap junction-mediated intracellular communication between osteocyte-like MLO-Y4 cells in responce to mechanical strain. Endocrinology 2001; 142: 3464-3473.
66. SAUNDERS MM, YOU J, TROSKO JE, YAMASAKI H, LI Z, DONAHUE HJ, JACOBS CR: Gap junctions and fluid flow response in MC3T3-E1 cells. Am J Physiol 2001; 281: C1917-C1925.
67. 2+ signaling pathway in osteoblasts. Am J Physiol 1998; 274: C1689-C1698.
68. IGARASHI K, WOO J-T, STERN PH: Effects of a selective cyclooxygenase-2 inhibitor, celecoxib, on bone resorption and osteoclastogenesis in vitro. Biochem Pharmacol 2002; 63: 523-32.
69. NIEDERBERGER E, TEGEDER I, VETTER G et al.: Celecoxib loses its anti-inflammatory efficacy at high doses through activation of NF-kB. FASEB J 2001; 15: 1622-1624
70. BUTTGEREIT F, SCREFFOLD, A: Rapid glucocorticoid effects on immune cells. Steroids 2002; 67: 529-34.
71. LETUVE S, DRUILHE A, GRANDSAIGNE M, AUBIER M, PRETOLANI M: Critical role of mitochondria, but not caspases, during glucocorticoid-induced human eosinophil apoptosis. Am J Respir Cell Mol Biol 2002; 26: 565-71.
72. BUTTGEREIT F, BURMESTER G-R, BRAND MD: Bioenergetics of immune functions: Fundamental and therapeutic aspects. Immunology Today 2000; 21, 189-196.
73. 2+-ATPase: A plausible link with its anti-tumour effect and cardiovascular risks. Biochem J 2002; 366: 831-7.
74. THUN MJ, HENLEY SJ, PATRONO C: Non-steroidal anti-inflammatory drugs as anti-cancer agents: Mechanistic, pharmacologic, and clinical issues. J Natl Cancer Inst 2002; 94: 252-266.
75. HE Q, LUO X, HUANG Y, SHEIKH MS: Apo2L/TRAIL differentially modulates the apoptotic effects of sulindac and a COX-2 selective non-steroidal anti-inflammatory agent in Bax-deficient cells. Oncogene 2002; 21: 6032-40.