Reverse engineering of long-chain polyunsaturated fatty acid biosynthesis into transgenic plants

European Journal of Lipid Science and Technology

Volumn 107, Issue 4, 2005, Pages 249-255

  Napier, Johnathan A ^a   Beaudoin, Frédéric ^a   Sayanova, Olga ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Desaturases; Elongases; Polyunsaturated fatty acids; Transgenic plants

Indexed keywords

ARACHIDONIC ACID; DOCOSAHEXAENOIC ACID; ICOSAPENTAENOIC ACID; POLYUNSATURATED FATTY ACID;

AMINO TERMINAL SEQUENCE; ENZYME ACTIVITY; FATTY ACID DESATURATION; FATTY ACID SYNTHESIS; GENE EXPRESSION; GENE FUNCTION; GENE IDENTIFICATION; GENETIC ENGINEERING; MICROSOME; NONHUMAN; OPEN READING FRAME; REVIEW; SEQUENCE HOMOLOGY; TRANSGENIC PLANT;

EID: 18244370256     PISSN: 14387697     EISSN: None     Source Type: Journal    
DOI: 10.1002/ejlt.200590022     Document Type: Review

References (30)

1. J. A. Napier: The production of long chain polyunsaturated fatty acids in transgenic plants: a sustainable source for human health and nutrition. Lipid Technol. 2004, 16, 103-107.
2. J. G. Wallis, J. L. Watts, J. Browse: Polyunsaturated fatty acid synthesis: what will they think of next? Trends Biochem Sci. 2002, 27, 467-470.
3. O. Sayanova, J. A. Napier: Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry. 2004, 65, 147-58.
4. A. P. Nugent: The metabolic syndrome. Nutrition Bull. 2004, 29, 36-43.
5. J. J. Thelen, J. B. Ohlrogge: Metabolic engineering of fatty acid biosynthesis in plants. Metab Eng. 2002, 4, 12-21.
6. 5 fusion desaturases in the synthesis of polyunsaturated fatty acids. Prostaglandins Leukot Essent Fatty Acids. 2003, 68, 135-143.
7. P. Sperling, P. Ternes, T. K. Zank, E. Heinz: The evolution of desaturases. Prostaglandins Leukot Essent Fatty Acids. 2003, 68, 73-95.
8. F. Beaudoin, L. V. Michaelson, S. J. Hey, M. J. Lewis, P. R. Shewry, O. Sayanova, J. A. Napier: Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway. Proc Natl Acad Sci USA. 2000, 97, 6421-6426.
9. J. M. Parker-Barnes, T. Das, E. Bobik, A. E. Leonard, J. M. Thurmond, L. T. Chaung, Y. S. Huang, P. Mukerji: Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids. Proc Natl Acad Sci USA. 2000, 97, 8284-8289.
10. A. A. Millar, L. Kunst: Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J. 1997, 12, 121-131.
11. 22 polyunsaturated fatty acids? Eur J Lipid Sci Technol. 2001, 103, 106-113.
12. J. G. Metz, P. Roessler, D. Facciotti, C. Levering, F. Dittrich, M. Lassner, R. Valentine, K. Lardizabal, F. Domergue, A. Yamada, K. Yazawa, V. Knauf, J. Browse: Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science. 2001, 293, 290-293.
13. J. A. Napier: Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms. Trends Plant Sci. 2002, 7, 51-54.
14. F. Domergue, J. Lerchl, U. Zähringer, E. Heinz: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis. Eur J Biochem. 2002, 269, 4105-4113.
15. H. Drexler, P. Spiekermann, A. Meyer, F. Domergue, T. Zank, P. Sperling, A. Abbadi, E. Heinz: Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. J Plant Physiol. 2001, 160, 779-802.
16. F. Domergue, A. Abbadi, C. Ott, T. K. Zank, U. Zähringer, E. Heinz: Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. J Biol Chem. 2003, 278, 35115-35126.
17. 18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS Lett. 2002, 510, 159-165.
18. 8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. Arch Biochem Biophys. 1999, 365, 307-316.
19. 9 polyunsaturated fatty acid specific elongase component alters Arabidopsis glycerolipid profiles. Plant Physiol. 2004, 135, 859-866.
20. A. A. Millar, M. Wrischer, L. Kunst: Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology. Plant Cell. 1998, 10, 1889-1902.
21. B. Qi, T. Fraser, S. Mugford, G. Dobson, O. Sayanova, J. Butler, J. Napier, A. K. Stobart, C. M. Lazarus: The production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in transgenic plants. Nature Biotechnol. 2004, 22, 739-745.
22. 5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canola. J Biol Chem. 1998, 273, 29360-29366.
23. S. Nakane, T. Tanaka, K. Satouchi, Y. Kobayashi, K. Waku, T. Sugiura: Occurrence of a novel cannabimimetic molecule 2-sciadonoylglycerol (2-eicosa-5′,11′,14′-trienoylglycerol) in the umbrella pine Sciadopitys verticillata seeds. Biol Pharm Bull. 2000, 23, 758-761.
24. A. G. Green: From alpha to omega-producing essential fatty acids in plants. Nature Biotechnol. 2004, 22, 680-682.
25. A. Abbadi, F. Domergue, A. Fahl, C. Ott, J. Bauer, J. A. Napier, R. Welti, P. Cirpus, E. Heinz: Biosynthesis of very long chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell. 2004, 16, 2734-2748.
26. T. J. Roscoe: Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics-based approach. Eur J Lipid Sci Technol. 2005, 107, 256-262.
27. F. Beaudoin, J. A. Napier: Biosynthesis and compartmentalisation of triacyglycerol in higher plants. Topics Curr Genetics. 2004, 6, 267-287.
28. A. Meyer, H. Kirsch, F. Domergue, A. Abbadi, P. Sperling, J. Bauer, P. Cirpus, T. K. Zank, H. Moreau, T. J. Roscoe, U. Zähringer, E. Heinz: Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J Lipid Res. 2004, 45, 1899-1909.
29. S. L. Pereira, A. E. Leonard, Y. S. Huang, L. T. Chuang, P. Mukerji: Identification of two novel microalgal enzymes involved in the conversion of the omega-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid. Biochem J. 2004, 384, 357-366.
30. H.-G. Opsahl-Ferstad, H. Rudi, B. Ruyter, S. Refstie: Biotechnological approaches to modify rapeseed oil composition for applications in aquaculture. Plant Sci. 2003, 165, 349-357.