Plasmid DNA adsorbed onto cationic microparticles mediates target gene expression and antigen presentation by dendritic cells

Gene Therapy

Volumn 7, Issue 24, 2000, Pages 2105-2112

  Denis Mize, K S ^a,b   Dupuis, M ^a,b   MacKichan, M L ^b   Singh, M ^b   Doe, B ^b   O'Hagan, D ^b   Ulmer, J B ^b   Donnelly, J J ^b   McDonald, D M ^a   Ott, G ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Cationic microparticles; Dendritic cells; DNA delivery; DNA vaccines

Indexed keywords

CATION; CATIONIC SURFACTANT; CETRIMIDE; DNA VACCINE; GAG PROTEIN; INTERLEUKIN 2; PLASMID DNA; POLYGLACTIN; POLYMER; PROTEIN P55;

ADSORPTION; ANIMAL CELL; ANTIGEN PRESENTATION; ARTICLE; BIODEGRADATION; BONE MARROW; CANCER IMMUNOTHERAPY; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENDRITIC CELL; DOSE RESPONSE; DRUG FORMULATION; ENDOSOME; FEMALE; FLUORESCENCE; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS; HYBRIDOMA; IN VITRO STUDY; IN VIVO STUDY; INTERNALIZATION; MACROPHAGE; MEDICAL RESEARCH; MOUSE; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STIMULATION; T LYMPHOCYTE; TECHNIQUE; VACCINE PRODUCTION;

ADSORPTION; ANALYSIS OF VARIANCE; ANIMALS; ANTIGEN PRESENTATION; CETRIMONIUM COMPOUNDS; DENDRITIC CELLS; FEMALE; GENE EXPRESSION; GENE PRODUCTS, GAG; GENE THERAPY; HYBRIDOMAS; IMAGE PROCESSING, COMPUTER-ASSISTED; INTERLEUKIN-2; LACTIC ACID; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; MICROSCOPY, CONFOCAL; MICROSPHERES; POLYGLYCOLIC ACID; POLYMERS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; T-LYMPHOCYTES; TRANSFECTION; VACCINES, DNA;

ANIMALIA; HUMAN IMMUNODEFICIENCY VIRUS; MURINAE;

EID: 17744380618     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301347     Document Type: Article

References (37)

1. The dendritic cells system and its role in immunogenicity
2. Dendritic cells and the control of immunity
3. Proliferating dendritic cell progenitors in human blood
4. Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application
5. Identification of proliferating dendritic cell precursors in mouse blood
6. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor
7. Dendritic cell vaccines for cancer immunotherapy
8. Bone marrow-derived dendritic cells pulsed with synthetic tumour peptides elicit protective and therapeutic antitumour immunity
9. Murine dendritic cells pulsed with whole tumor lysates mediate potent antitumor immune responses in vitro and in vivo
10. Dendritic cells retrovirally transduced with a model antigen gene are therapeutically effective against established pulmonary metastases
11. Dendritic cells genetically modified with an adenovirus vector encoding the cDNA for a model antigen induce protective and therapeutic antitumor immunity
12. Gene transfer in dendritic cells, induced by oral DNA vaccination with Salmonella typhimurium, results in protective immunity against a murine fibrosarcoma
13. Intratumoral injection of bone-marrow derived dendritic cells engineered to produce interleukin-12 induces complete regression of established murine transplantable colon adenocarcinomas
14. Targeting adenovirus
15. Attenuated, replication-competent herpes simplex virus type I mutant G207: Safety evaluation in mice
16. Design and application of HSV vectors for neuro-protection
17. A comparison of gene transfer methods in human dendritic cells
18. Nonviral transfection of distinct types of human dendritic cells: High efficiency gene transfer by electroporation into hematopoietic progenitor, but not monocyte-derived dendritic cells
19. Synthetic DNA delivery systems
20. Mechanisms of antigen uptake for presentation
21. Phagocytosis of polymer microspheres by macrophages
22. Cationic microparticles: A potent delivery system for DNA vaccines
23. Adsorption of oligonucleotides on to polyisohexylcyanoacrylate nanoparticles protects them against nucleases and increases their cellular uptake
24. Biodegradable polyalkylcyanoacrylate nanoparticles for the delivery of oligonucleotides
25. Increased expression and immunogenicity of sequence-modified human immunodeficiency virus type 1 gag gene
26. Comparative analysis of murine dendritic cells derived from spleen and bone marrow
27. LacZ inducible, antigen/MHC-specific T cell hybrids
28. Microspheres containing plasmid-encoded antigens elicit cytotoxic T-cell responses
29. Distribution of DNA vaccines determines their immunogenicity after intramuscular injection in mice
30. Cytoplasmic delivery of a fluorescent probe by poly(D,L lacticicoiglycolic acid) microspheres
31. DNA-PLGA microparticles: A promising delivery system for cancer gene therapy
32. DNA vaccination: Transfection and activation of dendritic cells as key events for immunity
33. Fully mobilizing host defense: Building better vaccines
34. Advances in vaccine adjuvants
35. Gene chemistry: Functionally and conformationally intact fluorescent plasmid DNA
36. HIV-1 p24 Gag-specific cytotoxic T-lymphocyte responses in mice
37. The T cell repertoire primed by antiviral vaccination is induced by self-tolerance