Gene chemistry: Functionally and conformationally intact fluorescent plasmid DNA

Human Gene Therapy

Volumn 10, Issue 1, 1999, Pages 15-24

  Zelphati, Olivier ^a   Liang, Xiaowu ^a   Hobart, Peter ^b   Felgner, Philip L ^a  

^a Genlantis Inc   (United States)

^b VICAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PEPTIDE NUCLEIC ACID; PLASMID DNA;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DNA BINDING; DNA CONFORMATION; DNA MODIFICATION; DNA TRANSFECTION; NONHUMAN; PROTEIN LOCALIZATION;

ANIMALS; CELL LINE; COS CELLS; DNA, SUPERHELICAL; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HAPLORHINI; LIPIDS; LIPOSOMES; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; NUCLEIC ACID CONFORMATION; PEPTIDE NUCLEIC ACIDS; PLASMIDS; RHODAMINES; SPECTROMETRY, FLUORESCENCE; THIONUCLEOTIDES; TRANSFECTION;

EID: 0032958001     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/10430349950019156     Document Type: Article

References (34)

1. ALMARSSON, O., BRUICE, T.C., KERR, J., and ZUCKERMANN, R.N. (1993). Molecular mechanics calculations of the structures of polyamide nucleic acid DNA duplexes and triple helical hybrids. Proc. Natl. Acad. Sci. U.S.A. 90, 7518-7522.
2. BENNETT, C.F., CHIANG, M.-Y., CHAN, H., SHOMAKER, J.E., and MIRABELLI, C.K. (1992). Cationic lipids enhance cellular uptke and activity of phosphorothioate antisense oligonucleotides. Mol. Pharmacol. 41, 1023-1033.
3. CHALFIE, M., TU, Y., EUSKIRCHEN, G., WARD, W.W., and PRASHER, D.C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
4. CHERNY, D.Y., BELOTSERKOVSKII, B.P., FRANK-KAMENETSKII, M.D., EGHOLM, M., BUCHARDT, O., BERG, R.H., and NIELSEN, P. (1993). DNA unwinding upon strand-displacement binding of a thymine-substituted polyamide to double-stranded DNA. Proc. Natl. Acad. Sci. U.S.A. 90, 1667-1670.
5. CHIN, D.J., GREEN, G.A., ZON, G., SZOKA, F.C., JR., and STRAUBINGER, R.M. (1990). Rapid nuclear accumulation of injected oligodeoxyribonucleotides. New Biol. 2, 1091-1100.
6. DEAN, D.A. (1997). Import of plasmid DNA into the nucleus is sequence specific. Exp. Cell Res. 230, 293-302.
7. DEMIDOV, V., FRANK-KAMENETSKII, M.D., EGHOLM, M., BUCHARDT, O., and NIELSEN, P. (1993). Sequence selective double strand DNA cleavage by peptide nucleic acid (PNA) targeting using nuclease S1. Nucleic Acids Res. 21, 2103-2107.
8. DEMIDOV, V.V., POTAMAN, V.N., FRANK-KAMENETSKII, M.D., EGHOLM, M., BUCHARDT, O., SONNICHSEN, S.H., and NIELSEN, P.E. (1994). Stability of peptide nucleic acids in human serum and cellular extracts. Biochem. Pharm. 48, 1310-1313.
9. DEMIDOV, V.V., YAVNILOVICH, M.V., BELOTSERKOVSKII, B.P., FRANK-KAMENETSKII, M.D., and NIELSEN, P. (1995). Kinetics and mechanism of polyamide ("peptide") nucleic acid binding to duplex DNA. Proc. Natl. Acad. Sci. U.S.A. 92, 2637-2641.
10. DOH, G.H., VAHLSING, L.H., HARTIKKA, J., LIANG, X., and MANTHORPE, M. (1997). Spatial-temporal patterns of gene expression in mouse skeletal muscle after injection of lacZ plasmid DNA. Gene Ther. 4, 648-663.
11. DOWTY, M.E., WILLIAMS, P., ZHANG, G., HAGSTROM, J.E., and WOLFF, J.A. (1995). Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc. Natl. Acad. Sci. U.S.A. 92, 4572-4576.
12. EGHOLM, M., NIELSEN, P., BUCHARDT, O., and BERG, R.H. (1992a). Recognition of guanine and adenine in DNA by cytosine and thymidine containing peptide nucleic acids (PNA). J. Am. Chem. Soc. 114, 9677-9678.
13. EGHOLM, M., BUCHARDT, O., NIELSEN, P., and BERG, R.H. (1992b). Peptide nucleic acids (PNA). Oligonucleotide analogues with an archiral peptide backbone. J. Am. Chem. Soc. 114, 1895-1897.
14. EGHOLM, M., BUCHARDT, O., CHRISTENSEN, L., BEHRENS, C., FREIER, S.M., DRIVER, D.A., BERG, R.H., KIM, S.K., NORDEN, B., and NIELSEN, P. (1993). PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. Nature (London) 365, 566-568.
15. EGHOLM, M., CHRISTENSEN, L., DUEHOLM, K.L., BUCHARDT, O., COULL, J., and NIELSEN, P.E. (1995). Efficient pH-indpendent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA. Nucleic Acids Res. 23, 217-222.
16. FELGNER, I.H., KUMAR, R., SRIDHAR, C.N., WHEELER, C.J., TSAI, Y.J., BORDER, R., RAMSEY, P., MARTIN, M., and FELGNER, P.L. (1994). Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. J. Biol. Chem. 269, 2550-2561.
17. FELGNER, P.L. (1996). Improvements in cationic liposomes for in vivo gene transfer. Hum. Gene Ther. 7, 1791-1793.
18. GAO, X., and HUANG, L. (1995). Cationic liposome-mediated gene transfer. Gene Ther. 2, 710-722.
19. GUSSONI, E., WANG, Y., FRAEFEL, C., MILLER, R.G., BLAU, H.M., GELLER, A.I., and KUNKEL, L.M. (1996). A method to codetect introduced genes and their products in gene therapy protocols. Nature Biotechnol. 14, 1012-1015.
20. HANVEY, J.C., PEFFER, N.J., BISI, J.E., THOMPSON, S.A., CADILLA, R., JOSEY, J.A., RICCA, D.J., HASSMAN, F., BONHAM, M.A., AU, K.G., CARTER, S.G., BRUCKENSTEIN, D.A., BOYD, A.L., NOBLE, S.A., and BABISS, L.E. (1992). Antisense and antigene properties of peptide nucleic acids. Science 258, 1481-1485.
21. HEIM, R., CUBITT, A.B., and TSIEN, R.Y. (1995). Improved green fluorescent. Nature (London) 373, 663-664.
22. LEONETTI, J.P., MECHTI, N., DEGOLS, G., GAGNOR, C., and LEBLEU, B. (1991). Intracellular distribution of microinjected antisense oligonucleotides. Proc. Natl. Acad. Sci. U.S.A. 88, 2702-2706.
23. LIANG, X., HARTIKKA, J., SUKHU, L., MANTHORPE, M., and HOBART, P. (1996). Novel, high expressing and antibiotic-controlled plasmid vectors designed for use in gene therapy. Gene Ther. 3, 350-356.
24. MERGNY, J.-L., GARESTIER, T., ROUGEE, M., LEBEDEV, A.V., CHASSIGNOL, M., THUONG, N.T., and HELENE, C. (1994). Fluorescence energy transfer between two triple helix-forming oligonucleotides bound to duplex DNA. Biochemistry 33, 15321-15328.
25. NIELSEN, P., EGHOLM, M., BERG, R.H., and BUCHARDT, O. (1991). Sequence-selective recognition of DNA by strand displacement with a thymidine-substituted polyamide. Science 254, 1497-1500.
26. NIELSEN, P., EGHOLM, M., and BUCHARDT, O. (1994). Sequence-specific transcription arrest by peptide nucleic acid bound to the DNA template strand. Gene 149, 139-145.
27. NORTON, J.C., MIECZYSLAW, A.P., WOODRING, E.W., SHAY, J.W., and COREY, D.R. (1996). Inhibition of human telomerase activity by peptide nucleic acids. Nature Biotechnol. 14, 615-619.
28. SIXOU, S., SZOKA, F.C., JR., GREEN, G.A., GIUSTI, B., ZON, G., and CHIN, D.J. (1994). Intracellular oligonucleotide hybridization detected by fluorescence resonance energy transfer (FRET). Nucleic Acids Res. 22, 662-668.
29. ULMER, J.B., DONNELLY, J.J., PARKER, S.E., RHODES, G.H., FELGNER, P.L., DWARKI, V.J., GROMKOWSKI, S.H., DECK, R.R., DEWITT, C.M., FRIEDMAN, A., HAWE, L.A., LEANDER, K.R., MARTINEZ, D., PERRY, H.C., SHIVER, J.W., MONTGOMERY, D.L., and LIU, M.A. (1993). Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259, 1745-1749.
30. VESELKOV, A.G., DEMIDOV, V.V., FRANK-KAMENETSKII, M.D., and NIELSEN, P. (1996). PNA as a rare genome-cutter. Mature (London) 379, 214.
31. WOLFF, J.A., MALONE, R.W., WILLIAMS, P., CHONG, W., ACSADI, G., JANI, A., and FELGNER, P.L. (1990). Direct gene transfer into mouse muscle in vivo. Science 247, 1495-1468.
32. ZABNER, J., FASBENDER, A.J., MONINGER, T., POELLINGER, K.A., and WELSH, M.J. (1995). Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem. 270, 18997-19007.
33. ZELPHATI, O., and SZOKA, F.C., JR. (1996a). Mechanism of oligonucleotide release from cationic liposomes. Proc. Natl. Acad. Sci. U.S.A. 93, 11493-11498.
34. ZELPHATI, O., and SZOKA JR., F.C. (1996b). Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids. Pharm. Res. 13, 1367-1372.