Biodegradation of Organophosphorus Pesticides by Surface-Expressed Organophosphorus Hydrolase

Nature Biotechnology

Volumn 15, Issue 10, 1997, Pages 984-987

  Richins, Richard D ^a   Kaneva, Irina ^a   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

Author keywords

Bioremediation; Immobilized bioreactor; Parathion

Indexed keywords

HYDROLASE; ORGANOPHOSPHATE INSECTICIDE;

ARTICLE; BIODEGRADATION; DETOXIFICATION; DRUG DEGRADATION; ENZYME ACTIVITY; PRIORITY JOURNAL;

BACTERIAL OUTER MEMBRANE PROTEINS; BIODEGRADATION, ENVIRONMENTAL; BIOREACTORS; BLOTTING, WESTERN; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; INSECTICIDES; MOLECULAR WEIGHT; PARAOXON; PARATHION; PHOSPHORIC MONOESTER HYDROLASES; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0030855956     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1097-984     Document Type: Article

References (28)

1. Munnecke, D.M. 1980. Enzymatic detoxification of waste organophosphate pesticides. J. Agric. Food Chem. 28:105-111.
2. Serdar, C.M. and Gibson, D.T. 1985. Enzymatic hydrolysis of organophosphates: Cloning and expression of a parathion hydrolase gene from Pseudomonas diminuta. Bio/Technology 3:567-571.
3. Mulbry, W.W., Karns, J.S., Kearney, P.C., Nelson, J.O., McDaniel, C.S., and Wild, J.R. 1986. Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by Southern hybridization with opd from Pseudomonas diminuta. Appl. Environ. Microbiol. 51:926-930.
4. Karns, J.S., Muldoon, M.T., Mulbury, W.W., Derbyshire, M.K., and Kearney, P.C. 1987. Use of microorganisms and microbial systems in the degradation of pesticides. pp. 156-170 in Biotechnology in agricultural chemistry. ACS symposium series. 334. LeBaron, H.M., Mumma, R.O., Honeycutt, R.C., and Duesing, J.H. (eds.) American Chemical Society, Washington, DC.
5. Attawav, H., Nelson, J.O., Baya, A.M., Voll, M.J., White, W.E., Grimes, D.J., and Colwell, R.R. 1987. Bacterial detoxification of diisopropyl fluorophosphate. Appl. Environ. Microbiol. 53:1685-1689.
6. Dumas, D.P., Durst, H.D., Landis, W.G., Raushel, F.M., and Wild, J.R. 1990. Inactivation of organophosphorus nerve agents by the phosphotriesterase from Pseudomonas diminuta. Arch. Biochem. Biophys. 227:155-159.
7. Dumas, D.P., Wild, J.R., and Raushel, F.M. 1989. Diisopropylfluorophosphate hydrolysis by a phosphotriesterase from Pseudomonas diminuta. Biotech. Appl. Biochem. 11:235-243.
8. Munnecke, D.M. 1979. Hydrolysis of organophosphate insecticides by an immobilized-enzyme system. Biotechnol. Bioeng. 21:2247-2261.
9. Caldwell, S.R. and Raushel, F.M. 1991. Detoxification of organophosphate pesticides using an immobilized phosphotriesterase from Pseudomonas diminuta. Biotechnol. Bioeng. 37:103-109.
10. Leduc, M., Frehel, C., and van Heijenoort, J. 1985. Correlation between degradation and ultrastructure of peptidoglycan during autolysis of Escherichia coli. J. Bacteriol. 161:627-635.
11. Marvin, H.J.P., ter Beest, M.B.A., and Witholt, B. 1989. Release of outer membrane fragments from wild-type Escherichia coli and from several E. coli lipopolysaccharide mutants by EDTA and heat shock treatment. J. Bacteriol. 179:5262-5267.
12. Francisco, J.A., Earhart, C.F., and Georgiou, G. 1992. Transport and anchoring of β-lactamase to the external surface of Escherichia coli. Proc. Natl. Acad. Sci. USA 89:2713-2717.
13. McDaniel, C.S., Harper, L.L., and Wild, J.R. 1988. Cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase. J. Bacteriol. 170:2306-2311.
14. Serdar, C.M., D.C. Murdock, and M.F. Rohde. 1989. Parathion hydrolase gene form Pseudomonas diminuta MG: Subcloning, complete nucleotide sequence, and expression of the mature portion of the enzyme in Escherichia coli. Bio/Technol. 7:1151-1155.
15. Dumas, D.P., Caldwell, S.R., Wild, J.R., and Raushel, F.M. 1989. Purification and properties of the phosphotriesterase from Pseudomonas diminuta. J. Biol. Chem. 33:19659-19665.
16. Phillips, J.P., Xin, J.H., Kirby, K., Milne, C.P., Krell, P., and Wild, J.R. 1990. Transfer and expression of an organophosphate insecticide-degrading gene from Pseudomonas in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 87: 8155-8159.
17. Steiert, J.G., Pogell, B.M., Speedie, M.K., and Laredo, J. 1989. Agene coding for a membrane-bound hydrolase is expressed as a secreted soluble enzyme in Streptomyces lividans. Bio/Technology 7:65-68.
18. Dave, K.I., Miller, C.E., and Wild, J.R. 1993. Characterization of organophosphorus hydrolases and the genetic manipulation of the phosphotriesterase from Pseudomonas diminuta. Chem.-Biol. Interactions 87:55-68.
19. Francisco, J.A., Stathopoulos, C., Warren, R.A.J., Kilburn, D.G., and Georgiou, G. 1993. Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Bio/Technology 11:491-495.
20. Bowden, G.A., Paredes, A.M., and Georgiou, G. 1991. Structure and morphology of protein inclusion bodies in Escherichia coli. Bio/Technology 9:725-730.
21. Bukau, B. 1993. Regulation of the Escherichia coli heat-shock response. Mol. Microbiol. 9:671-680.
22. Mulbry, W.W. and Karns, J.S. 1989. Parathion hydrolase specified by the Flavobacterium opd gene: Relationship between the gene and protein. J. Bacteriol. 171:6740-6746.
23. Stathopoulos, C., Georgiou, G., and Earhart, C.F. 1996. Characterization of Escherichia coli expressing an Lpp’OmpA(46-159)-PhoA fusion protein localized in the outer membrane. Appl. Microbiol. Biotechnol. 45:112-119.
24. Cordes, C., Meima, R., Twiest, B., Kazemier, B., Venema, G., van Dijl, J.M., and Bron, S. 1996. The expression of a plasmid-specified exported protein causes structural plasmid instability in Bacillus subtilis. J. Bacteriol. 178: 5235-5242.
25. Sambrook, J., Fritsch, E.F., Maniatis, T. 1989. Molecular cloning—a laboratory manual, 2nd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
26. Currier, T.C. and Nester, E.W. 1976. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal. Biochem. 76:431-441.
27. Strauch, M.A., Spiegelman, G.B., Perego, M., Johnson, W.C., Burbulys, D., and Hoch, J.A. 1989. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 8:1615-1621.
28. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.