ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway

Nature Cell Biology

Volumn 6, Issue 2, 2004, Pages 121-128

  Ohtsuka, Takao ^a   Ryu, Hoon ^b   Minamishima, Yohji A ^a   Macip, Salvador ^c   Sagara, Junji ^d   Nakayama, Keiichi I ^e   Aaronson, Stuart A ^c   Lee, Sam W ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^d SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^e KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTOR PROTEIN; CASPASE 2; CASPASE 3; CASPASE 9; CYTOCHROME C; PROTEIN BAX; PROTEIN P53; SMALL INTERFERING RNA; BAX PROTEIN, HUMAN; CASPASE; CYTOSKELETON PROTEIN; HYBRID PROTEIN; ONCOPROTEIN; PROTEIN BCL 2; PYCARD PROTEIN, HUMAN;

APOPTOSIS; ARTICLE; CELL LINE; CELL MEMBRANE POTENTIAL; CONTROLLED STUDY; ENZYME ACTIVATION; GENOTOXICITY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; STRESS; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; TUMOR CELL LINE;

APOPTOSIS; BASE SEQUENCE; BCL-2-ASSOCIATED X PROTEIN; CASPASES; CELL LINE, TUMOR; CYTOCHROMES C; CYTOSKELETAL PROTEINS; ENZYME ACTIVATION; HUMANS; MEMBRANE POTENTIALS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT FUSION PROTEINS; RNA, SMALL INTERFERING; TUMOR SUPPRESSOR PROTEIN P53;

EID: 1642633791     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1087     Document Type: Article

References (30)

1. Pawlowski, K., Pio, F., Chu, Z., Reed, J. C. & Godzik, A. PAAD - a new protein domain associated with apoptosis, cancer and autoimmune diseases. Trends Biochem. Sci. 26, 85-87 (2001).
2. Masumoto, J. et al. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J. Biol. Chem. 274, 33825-33838 (1999).
3. Conway, K. E. et al. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 60, 6236-6242 (2000).
4. Stehlik, C. et al. The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor κB activation pathways. J. Exp. Med. 196, 1605-1615 (2002).
5. Chae, J. J. et al. Targeted disruption of Pyrin, the FMF protein, causes heightened sensitivity to endotoxin and a defect in macrophage. Mol. Cell 11, 591-604 (2003).
6. McConnell, B. B. & Vertino, P. M. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res. 60, 6243-6247 (2000).
7. Shiohara, M. et al. ASC, which is composed of a PYD and a CARD, is up-regulated by inflammation and apoptosis in human neutrophils. Biochem. Biophys. Res. Commun. 293, 1314-1318 (2002).
8. Vogelstein, B., Lane, D. & Levine, A. J. Surfing the p53 network. Nature 408, 307-310 (2000).
9. Oren, M. Decision making by p53: Life, death and cancer. Cell Death Differ. 10, 431-442 (2003).
10. Miyashita, T. & Reed, J. C. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80, 293-299 (1995).
11. Wei, M. C. et al. Proapoptotic BAX and BAK: A requisite gateway to mitochondrial dysfunction and death. Science 292, 727-730 (2001).
12. Gross, A., McDonnell, J. M. & Korsmeyer, S. J. BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 13, 1899-1911 (1999).
13. Zhang, L., Yu, J., Park, B. H., Kinzler, K. W. & Vogelstein, B. Role of Bax in the apoptotic response to anticancer agents. Science 290, 989-992 (2000).
14. Fearnhead, H. O. et al. Oncogene-dependent apoptosis is mediated by caspase-9. Proc. Natl Acad. Sci. U S A 95, 13664-13669 (1998).
15. Lindsten, T. et al. The combined functions of proapoptotic Bcl-2 family members bak and bax are essential for normal development of multiple tissues. Mol. Cell 6, 1389-1399 (2000).
16. Zong, W. X., Lindsten, T., Ross, A. J., MacGregor, G. R. & Thompson, C. B. BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev. 15, 1481-1486 (2001).
17. Li, H. & Yuan, J. Deciphering the pathways of life and death. Curr. Opin. Cell Biol. 11, 261-266 (1999).
18. Coultas, L. & Strasser, A. The role of the Bcl-2 protein family in cancer. Semin. Cancer Biol. 13, 115-123 (2003).
19. Green, D. R. & Reed, J. C. Mitochondria and apoptosis. Science 281, 1309-1312 (1998).
20. Hsu, Y. T. & Youle, R. J. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J. Biol. Chem. 273, 10777-10783 (1998).
21. Nechushtan, A., Smith, C. L., Hsu, Y. T. & Youle, R. J. Conformation of the Bax C-terminus regulates subcellular location and cell death. EMBO J. 18, 2330-2341 (1999).
22. Suzuki, M., Youle, R. J. & Tjandra, N. Structure of Bax: coregulation of dimer formation and intracellular localization. Cell 103, 645-654 (2000).
23. Marsden, V. S. et al. Apoptosis initiated by Bcl-2-regulated caspase activation independently of the cytochrome c/Apaf-1/caspase-9 apoptosome. Nature 419, 634-637 (2002).
24. Masumoto, J. et al. ASC is an activating adaptor for NF-κB and caspase-8-dependent apoptosis. Biochem. Biophys. Res. Commun. 303, 69-73 (2003).
25. Lassus, P., Opitz-Araya, X. & Lazebnik, Y. Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297, 1352-1354 (2002).
26. Sawada, M. et al. Ku70 suppresses the apoptotic translocation of Bax to mitochondria. Nature Cell Biol. 5, 320-329 (2003).
27. Guo, B. et al. Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature 423, 456-461 (2003).
28. Stimson, K. M. & Vertino, P. M. Methylation-mediated silencing of TMS1/ASC is accompanied by histone hypoacetylation and CpG island-localized changes in chromatin architecture. J. Biol. Chem. 277, 4951-4958 (2002).
29. Masumoto, J., Taniguchi, S. & Sagara, J. Pyrin N-terminal homology domain- and caspase recruitment domain-dependent oligomerization of ASC. Biochem. Biophys. Res. Commun. 280, 652-655 (2001).
30. He, T. et al. A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95, 2509-2514 (1998).